QBM EXAM 4 QUESTIONS AND ANSWERS
Which of the following expression systems is fastest, but would not glycosylate a protein? - CORRECT
ANSWER✅✅Bacteria
Transfection - CORRECT ANSWER✅✅can be performed using the capabilities of retroviruses.
__________ is a form of post-transcriptional gene regulation in which non-translated double-stranded
RNA (dsRNA) molecules called small interfering RNA (siRNA) mediate sequence specific degradation of
target messenger RNA (mRNA). - CORRECT ANSWER✅✅RNA interference
Cells can be directly modified via __________, which involves using a small glass pipette or microscopic
lance to add or remove nuclear material (enucleation) or inject molecules into a cell, through the plasma
membrane. - CORRECT ANSWER✅✅microinjection
Immunofluorescence is most similar to performing - CORRECT ANSWER✅✅a western blot.
Which of the following techniques would be best suited to directly study cellular metabolism in vitro? -
CORRECT ANSWER✅✅Pulse-chase.
In FACS, cells can be separated based on - CORRECT ANSWER✅✅stage of cell cycle.
size.
health.
number of chromosomes.
In flow cytometry, the forward scattering of light can indicate __________, and the side scatter indicates
__________. - CORRECT ANSWER✅✅the size of a cell ; the cell's granularity, or health
During in situ organ perfusion, - CORRECT ANSWER✅✅an organ is removed from the model organism,
maintained at constant temperature and pH in an apparatus, and fluid is passed through the organ.
, Which of the following is a benefit of lower-order model organisms versus higher-order model
organisms? - CORRECT ANSWER✅✅Short development time.
Any investigator wishing to use animals in a research program must first submit a detailed animal
protocol to __________ for approval. - CORRECT ANSWER✅✅the IACUC committee
Which of the following statements concerning transgenic animals is correct? - CORRECT
ANSWER✅✅Pigs with human-sized organs have been designed, to aid in the supply of transplant
organs.
The incorporation of GFP into the genome of animals results in the creation of a transgenic animal.
Transgenic animals have been used to make model organisms for human illnesses, by mimicking
phenotypes.
Transgenic animals have non-native genomes, or genomes that have been modified.
(ALL)
RNAi can only be used in cell culture, not in vivo - CORRECT ANSWER✅✅False
Cloning - CORRECT ANSWER✅✅NOT used to involve iPSCs, but now is mostly performed directly.
NOT is now performed by inserting the nucleus of the cell to be cloned into the uterus directly.
What is the benefit of using knockdown mice instead of knockout mice? - CORRECT ANSWER✅✅The
mutation may be embryonic lethal; knockdown would allow to still assess the effects of the gene.
A knock-in mouse - CORRECT ANSWER✅✅has a gene insertion.
Which of the following best describes the Cre-Lox system? - CORRECT ANSWER✅✅A transgenic mouse
that knocks out a target gene only in a specific cell type, by utilizing gene inversion.
Which of the following either can be performed now or may be possible in the future using CRISPR? -
CORRECT ANSWER✅✅Repair diseases caused by point mutations.
Bring the wooly mammoth back from extinction.
Which of the following expression systems is fastest, but would not glycosylate a protein? - CORRECT
ANSWER✅✅Bacteria
Transfection - CORRECT ANSWER✅✅can be performed using the capabilities of retroviruses.
__________ is a form of post-transcriptional gene regulation in which non-translated double-stranded
RNA (dsRNA) molecules called small interfering RNA (siRNA) mediate sequence specific degradation of
target messenger RNA (mRNA). - CORRECT ANSWER✅✅RNA interference
Cells can be directly modified via __________, which involves using a small glass pipette or microscopic
lance to add or remove nuclear material (enucleation) or inject molecules into a cell, through the plasma
membrane. - CORRECT ANSWER✅✅microinjection
Immunofluorescence is most similar to performing - CORRECT ANSWER✅✅a western blot.
Which of the following techniques would be best suited to directly study cellular metabolism in vitro? -
CORRECT ANSWER✅✅Pulse-chase.
In FACS, cells can be separated based on - CORRECT ANSWER✅✅stage of cell cycle.
size.
health.
number of chromosomes.
In flow cytometry, the forward scattering of light can indicate __________, and the side scatter indicates
__________. - CORRECT ANSWER✅✅the size of a cell ; the cell's granularity, or health
During in situ organ perfusion, - CORRECT ANSWER✅✅an organ is removed from the model organism,
maintained at constant temperature and pH in an apparatus, and fluid is passed through the organ.
, Which of the following is a benefit of lower-order model organisms versus higher-order model
organisms? - CORRECT ANSWER✅✅Short development time.
Any investigator wishing to use animals in a research program must first submit a detailed animal
protocol to __________ for approval. - CORRECT ANSWER✅✅the IACUC committee
Which of the following statements concerning transgenic animals is correct? - CORRECT
ANSWER✅✅Pigs with human-sized organs have been designed, to aid in the supply of transplant
organs.
The incorporation of GFP into the genome of animals results in the creation of a transgenic animal.
Transgenic animals have been used to make model organisms for human illnesses, by mimicking
phenotypes.
Transgenic animals have non-native genomes, or genomes that have been modified.
(ALL)
RNAi can only be used in cell culture, not in vivo - CORRECT ANSWER✅✅False
Cloning - CORRECT ANSWER✅✅NOT used to involve iPSCs, but now is mostly performed directly.
NOT is now performed by inserting the nucleus of the cell to be cloned into the uterus directly.
What is the benefit of using knockdown mice instead of knockout mice? - CORRECT ANSWER✅✅The
mutation may be embryonic lethal; knockdown would allow to still assess the effects of the gene.
A knock-in mouse - CORRECT ANSWER✅✅has a gene insertion.
Which of the following best describes the Cre-Lox system? - CORRECT ANSWER✅✅A transgenic mouse
that knocks out a target gene only in a specific cell type, by utilizing gene inversion.
Which of the following either can be performed now or may be possible in the future using CRISPR? -
CORRECT ANSWER✅✅Repair diseases caused by point mutations.
Bring the wooly mammoth back from extinction.
