Page 1 of 46
BIO 181 LAB FINAL EXAM NEWEST VERSION WITH
COMPLETE QUESTIONS AND CORRECT DETAILED
SOLUTIONS LATEST UPDATED VERSION JUST RELEASED
Question: what is the smallest objective on a microscope called? - CORRECT
ANSWER✔✔scanning objective
Question: primary carbohydrate in our diet? primary lipid? primary protein? - CORRECT
ANSWER✔✔starch is primary carb, glucose is primary carb we absorb and use for energy
triglyceride is primary lipid
amino acids are where we get our primary protein
Question: what are examples of reducing sugars in the benedicts test for simple sugars
experiment? - CORRECT ANSWER✔✔glucose and fructose (monosaccarides)
Question: in the benedict's test for simple sugars, Benedict's reagent is mixed with a sugar in
the experiment. what is benedict's reagent? - CORRECT ANSWER✔✔Copper (Cu)
, Page 2 of 46
Question: in benedict's test for simple sugars, the sugar added to the solution begins as what
and ends as what? (reduced vs oxidized) - CORRECT ANSWER✔✔reduced sugar --> oxidized
sugar
Question: in the iodine test for starch, which polymer will iodine react with and which will it
not react with? - CORRECT ANSWER✔✔it will react with starch (turning blue) but does not react
with non-coiled carbs, monosaccharides, and disaccharide's (remains yellow-brown)
Question: in biuret's test for protein what is Biuret reagent (Cu2+) complexed with? - CORRECT
ANSWER✔✔peptide bonds, it will produce a violet color when it reacts
Question: In sudan IV test for lipids, Sudan IV is a fat-soluble dye, therefore its pigments will
only be absorbed by a lipid. what color will it turn when it reacts? - CORRECT ANSWER✔✔dark
red
Q:which microscope has a two-lens system? - CORRECT ANSWER✔✔compound
, Page 3 of 46
Question: what is the piece of microscope you look through? - CORRECT ANSWER✔✔ocular
Question: which of the parts of a microscope houses the second lens system (magnification is
written on it) on the microscope used today? - CORRECT ANSWER✔✔objectives (10x, 40x, 100x)
Question: to increase or decrease the amount of light coming through the stage, this part is
used. - CORRECT ANSWER✔✔the diaphragm
Question: a microscope's ability to remain in focus no matter what objective is used is termed:
- CORRECT ANSWER✔✔parfocal
Question: which objectives should you always start with when you begin using the
microscope? - CORRECT ANSWER✔✔the lowest
Question: when you increase the magnification, it usually does what to the field of vision? -
CORRECT ANSWER✔✔decreases
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Question: what is another name for a stereomicroscope - CORRECT ANSWER✔✔dissecting
scope
Question: the best word used to describe the thickness of the plane of focus is: - CORRECT
ANSWER✔✔depth of focus
Question: when determining sensitivity of each assay, what characteristic are you testing? -
CORRECT ANSWER✔✔lowest concentration
Question: Negative control vs. positive control - CORRECT ANSWER✔✔negative: used to show
that any reaction is due to a sample and not something contaminating a test; a known-result
(water is often used)
positive: used to show that the test is working properly
BIO 181 LAB FINAL EXAM NEWEST VERSION WITH
COMPLETE QUESTIONS AND CORRECT DETAILED
SOLUTIONS LATEST UPDATED VERSION JUST RELEASED
Question: what is the smallest objective on a microscope called? - CORRECT
ANSWER✔✔scanning objective
Question: primary carbohydrate in our diet? primary lipid? primary protein? - CORRECT
ANSWER✔✔starch is primary carb, glucose is primary carb we absorb and use for energy
triglyceride is primary lipid
amino acids are where we get our primary protein
Question: what are examples of reducing sugars in the benedicts test for simple sugars
experiment? - CORRECT ANSWER✔✔glucose and fructose (monosaccarides)
Question: in the benedict's test for simple sugars, Benedict's reagent is mixed with a sugar in
the experiment. what is benedict's reagent? - CORRECT ANSWER✔✔Copper (Cu)
, Page 2 of 46
Question: in benedict's test for simple sugars, the sugar added to the solution begins as what
and ends as what? (reduced vs oxidized) - CORRECT ANSWER✔✔reduced sugar --> oxidized
sugar
Question: in the iodine test for starch, which polymer will iodine react with and which will it
not react with? - CORRECT ANSWER✔✔it will react with starch (turning blue) but does not react
with non-coiled carbs, monosaccharides, and disaccharide's (remains yellow-brown)
Question: in biuret's test for protein what is Biuret reagent (Cu2+) complexed with? - CORRECT
ANSWER✔✔peptide bonds, it will produce a violet color when it reacts
Question: In sudan IV test for lipids, Sudan IV is a fat-soluble dye, therefore its pigments will
only be absorbed by a lipid. what color will it turn when it reacts? - CORRECT ANSWER✔✔dark
red
Q:which microscope has a two-lens system? - CORRECT ANSWER✔✔compound
, Page 3 of 46
Question: what is the piece of microscope you look through? - CORRECT ANSWER✔✔ocular
Question: which of the parts of a microscope houses the second lens system (magnification is
written on it) on the microscope used today? - CORRECT ANSWER✔✔objectives (10x, 40x, 100x)
Question: to increase or decrease the amount of light coming through the stage, this part is
used. - CORRECT ANSWER✔✔the diaphragm
Question: a microscope's ability to remain in focus no matter what objective is used is termed:
- CORRECT ANSWER✔✔parfocal
Question: which objectives should you always start with when you begin using the
microscope? - CORRECT ANSWER✔✔the lowest
Question: when you increase the magnification, it usually does what to the field of vision? -
CORRECT ANSWER✔✔decreases
, Page 4 of 46
Question: what is another name for a stereomicroscope - CORRECT ANSWER✔✔dissecting
scope
Question: the best word used to describe the thickness of the plane of focus is: - CORRECT
ANSWER✔✔depth of focus
Question: when determining sensitivity of each assay, what characteristic are you testing? -
CORRECT ANSWER✔✔lowest concentration
Question: Negative control vs. positive control - CORRECT ANSWER✔✔negative: used to show
that any reaction is due to a sample and not something contaminating a test; a known-result
(water is often used)
positive: used to show that the test is working properly