B io 205 lab fi nal w w u Qu estions
an d answ er s w ith accu r ate
r ationales(PASSED)
If you wanted 650mL of 45mM NaCL from a 0.8M stock solution, how would you
make it? Explain the process in 1-2 sentences and please include units on your
answers. - answer
what is the purpose of a blank for a spectrophotometer? - answer A blank solution
has no analyte of interest and allows us to set the spectrophotometer to 0 before
we measure the "unknown" solution
what are the minimum and maximum volumes a p20, p200, and p1000 can
pipette? - answer p20-max:20uL min: 2
p200- max: 200uL min: 20uL
p1000- max: 1000uL min: 100uL
what are primary, secondary, tertiary, and quaternary structures? - answer
Primary: the sequence of amino acids in the polypeptide chain
Secondary: the substructure produced by the backbone of the amino acid chain
Tertiary: the 3-D structure of an individual protein
Quaternary: the 3-D structure of two or more individual proteins that operate as a
single unit
, what interactions/bonds stabilize each structure - answer primary- covalent bonds
(peptide bonds)
secondary: hydrogen bonds
Tertiary and quaternary: : ionic, disulfide, hydrophobic interactions, hydrogen, and
van der Waals
what is the function of an enzyme? - answer They are biological catalysts
How can enzymes denature - answer unfavorable pH, temp, concentration of
metals/cations
what does turnover number measure? - answer the # of substrate molecules
converted to product molecules per enzyme molecule unit time
turnover rate = rate of reaction/enzyme concentration
what are hypertonic, hypotonic, and isotonic solutions? - answer hypertonic:
solutions with a high osmotic pressure. (shrink)
hypotonic: solutions are solutions having a lower osmotic pressure (swell)
isotonic: solutions having equal osmotic pressures (same)
what part does kinetic energy play in diffusion? - answer provides the random
motion of molecules that allows them to move from an area of high concentration
to an area of low concentration
what is an ocular micrometer used for and why do we need to calibrate it? -
answer is a glass disc on which a series of uniformly spaced lines has been
an d answ er s w ith accu r ate
r ationales(PASSED)
If you wanted 650mL of 45mM NaCL from a 0.8M stock solution, how would you
make it? Explain the process in 1-2 sentences and please include units on your
answers. - answer
what is the purpose of a blank for a spectrophotometer? - answer A blank solution
has no analyte of interest and allows us to set the spectrophotometer to 0 before
we measure the "unknown" solution
what are the minimum and maximum volumes a p20, p200, and p1000 can
pipette? - answer p20-max:20uL min: 2
p200- max: 200uL min: 20uL
p1000- max: 1000uL min: 100uL
what are primary, secondary, tertiary, and quaternary structures? - answer
Primary: the sequence of amino acids in the polypeptide chain
Secondary: the substructure produced by the backbone of the amino acid chain
Tertiary: the 3-D structure of an individual protein
Quaternary: the 3-D structure of two or more individual proteins that operate as a
single unit
, what interactions/bonds stabilize each structure - answer primary- covalent bonds
(peptide bonds)
secondary: hydrogen bonds
Tertiary and quaternary: : ionic, disulfide, hydrophobic interactions, hydrogen, and
van der Waals
what is the function of an enzyme? - answer They are biological catalysts
How can enzymes denature - answer unfavorable pH, temp, concentration of
metals/cations
what does turnover number measure? - answer the # of substrate molecules
converted to product molecules per enzyme molecule unit time
turnover rate = rate of reaction/enzyme concentration
what are hypertonic, hypotonic, and isotonic solutions? - answer hypertonic:
solutions with a high osmotic pressure. (shrink)
hypotonic: solutions are solutions having a lower osmotic pressure (swell)
isotonic: solutions having equal osmotic pressures (same)
what part does kinetic energy play in diffusion? - answer provides the random
motion of molecules that allows them to move from an area of high concentration
to an area of low concentration
what is an ocular micrometer used for and why do we need to calibrate it? -
answer is a glass disc on which a series of uniformly spaced lines has been
