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Which of the following are advantages to treating diabetics
with genetically engineered human insulin, rather than pig or
cow insulin? - ANSWER-There is less chance of an immune
response to the human insulin. Ethical issues are avoided
concerning the use of animals.
It is a more efficient process to purify insulin from yeast or
bacteria cultures than it is from animal organs
"Bioethics" is best described as: - ANSWER-The study of
decision making as it applies to moral decisions that must be
made due to advances in biology and technology.
Which of the following are permitted in the laboratory? -
ANSWER-Eye glasses
Which of the following is NOT part of manufacturing a
biopharmaceutical drug?
- ANSWER-EPA
review
cGMP is an abbreviation used in the pharmaceutical and
biopharmaceutical
,industry, and stands for: - ANSWER-Current Good
Manufacturing Practices
Which of the following is a critical function of Quality
Assurance (QA) personnel? - ANSWER-Maintain and control
documentation for regulatory compliance
The agency responsible for regulating biopharmaceutical
drugs for use in
humans is: - ANSWER-Food & Drug
Administration (FDA)
What is the chance a child will be born with cystic fibrosis (a
homozygous recessive trait (aa)) if both the mother and father
are heterozygous (Aa)? - ANSWER-25% chance of having the
disorder.
The observable traits of an organism are referred to as
the: - ANSWER-
Phenotype
If DNA segments changes from GGTTAG to GGATAG this is
a: - ANSWER-
Nucleotide base
substitution
Which of the strategies below could be used for ligating the
genomic DNA into
, the EcoRI site in the plasmid? - ANSWER-Cut the genomic
DNA again with
EcoRI and insert the DNA fragment into the plasmid
cut with EcoRI.
How many grams is 2.6kg? - ANSWER-2600
Plasmid DNA is purified using anion exchange column
chromatography. Why? - ANSWER-Because DNA is negatively
charged, binds to the positively charged resin, and is eluted
with increasing salt.
Following a restriction digest of plasmid DNA, you anticipate
having of 4 DNA fragments that are very close in size (between
700 and 1000 bp). Which of the following will increase the
chances for good separation on an agarose gel, so that you
may visualize both bands on the gel? - ANSWER-Use a higher
percentage of agarose (1.5 % or higher) when making the gel
Which of the following will change the resistance of the
electrophoresis unit
being run at a constant voltage? - ANSWER-Amount of buffer,
Thickness of agarose gel and
Percentage of agarose in gel
Which of the following principle(s) result in DNA molecules
being separated on an agarose gel? - ANSWER-DNA moves
toward the positive electrode due to negative charge of