BACTERIAL IDENTIFICATION VIRTUAL LAB
PRACTICE REVIEW 2026 COMPLETE
VERIFIED SOLUTIONS
⩥ Prepare a sample and isolate the whole DNA, make copies of it,
sequence the DNA, analyze and identify Answer: What are the four
basic steps involved in this bacterial identification lab?
⩥ The piece of DNA used for identifying bacteria; The identification
relies on matching the sequence from your sample against a database
of all known 16S rDNA sequences Answer: What is "16S rDNA," and
how is it used to identify species of bacteria?
⩥ to identify a bacterial sample received from a clinician. Answer: As
the pathology lab technician, what is your task in this virtual lab?
⩥ dissolving the cell wall with a digestive buffer (in the white-capped
bottle) available as a commercial kit Answer: Extracting DNA
involves which initial step?
⩥ Answer: What is the wire ring used for?
⩥ The buffer contains proteolytic enzymes that eat the cell wall.
Answer: Why are the proteolytic enzymes necessary?
, ⩥ Since we will be using other enzymes in the next step, we need to
get rid of the proteolytic enzymes. Answer: Why do you then need to
inactivate the proteolytic enzymes and how do you do it?
⩥ DNA is lighter than the cellular debris floating around from the step
that destroyed the cellular wall. The centrifuge allows us to safely
remove the DNA from the microcentrifuge tube. Answer: After
removing the enzymes, why do you spin the sample in the centrifuge?
⩥ a solid deposit of cellular debris Answer: What is the pellet?
⩥ the liquid Answer: What is the supernatant?
⩥ the DNA is contained in the supernatant Answer: Where is the
DNA?
⩥ Polymerase Chain Reactant; makes copies of DNA Answer: What
does "PCR" stand for and what is the purpose of PCR?
⩥ Answer: Summarize the process of PCR in a diagram. Include all
the steps, labeled and in the right order.
⩥ water, a buffer to maintain pH, nitrogenous bases Answer: What
does the Master Mix contain?
PRACTICE REVIEW 2026 COMPLETE
VERIFIED SOLUTIONS
⩥ Prepare a sample and isolate the whole DNA, make copies of it,
sequence the DNA, analyze and identify Answer: What are the four
basic steps involved in this bacterial identification lab?
⩥ The piece of DNA used for identifying bacteria; The identification
relies on matching the sequence from your sample against a database
of all known 16S rDNA sequences Answer: What is "16S rDNA," and
how is it used to identify species of bacteria?
⩥ to identify a bacterial sample received from a clinician. Answer: As
the pathology lab technician, what is your task in this virtual lab?
⩥ dissolving the cell wall with a digestive buffer (in the white-capped
bottle) available as a commercial kit Answer: Extracting DNA
involves which initial step?
⩥ Answer: What is the wire ring used for?
⩥ The buffer contains proteolytic enzymes that eat the cell wall.
Answer: Why are the proteolytic enzymes necessary?
, ⩥ Since we will be using other enzymes in the next step, we need to
get rid of the proteolytic enzymes. Answer: Why do you then need to
inactivate the proteolytic enzymes and how do you do it?
⩥ DNA is lighter than the cellular debris floating around from the step
that destroyed the cellular wall. The centrifuge allows us to safely
remove the DNA from the microcentrifuge tube. Answer: After
removing the enzymes, why do you spin the sample in the centrifuge?
⩥ a solid deposit of cellular debris Answer: What is the pellet?
⩥ the liquid Answer: What is the supernatant?
⩥ the DNA is contained in the supernatant Answer: Where is the
DNA?
⩥ Polymerase Chain Reactant; makes copies of DNA Answer: What
does "PCR" stand for and what is the purpose of PCR?
⩥ Answer: Summarize the process of PCR in a diagram. Include all
the steps, labeled and in the right order.
⩥ water, a buffer to maintain pH, nitrogenous bases Answer: What
does the Master Mix contain?
