Drexel bio 209 final Exam, Most Recent Exam 2026
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Select all of the following that are considered when
assigning a biosafety risk level to a microbe. - Answers--
Infectivity
-Severity of Disease Caused
-Transmissibility
Which person is working in a BSL-4 facility? - Answers-1
Most of the microorganisms we will use this semester in
BIO209 are risk level ____ but we will use some risk level
___ microbes. - Answers-1,2
Which of these are required to participate in person in
microbiology laboratory? - Answers--lab coat
-disposable gloves
-closed toed shoes
Answer: all of the above
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Outbreaks of infectious diseases linked to microbes used
in clinical and teaching microbiology labs have led to
hospitalizations and deaths. - Answers-True
Which of the following are true of Biosafety Level - 2 (BSL-
2)? - Answers--BSL-2 requirements are more stringent
than BSL-1 requirements.
-A microbe that is typically worked with at a BSL-2
laboratory is Staphylococcus aureus.
-Appropriate personal protective equipment (PPE) is worn,
including lab coats and gloves. Eye protection and face
shields can also be worn, as needed.
-If you work in a lab that is designated a BSL-2, the
microbes there pose moderate hazards to laboratorians
and the environment. The microbes are typically
indigenous and associated with diseases of varying
severity.
Select the TRUE statements from the list - Answers--
Overheated loops can kill the bacteria used for plate
streaking.
-Plates need to be placed in the incubator with the media
side up.
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-You should always disinfect your benches before starting
an experiment.
What is wrong with this streak plate? - Answers-Individual
colonies are not isolated and growth doesn't decrease in
subsequent quadrants.
When streaking a plate, how many times does the loop
come into contact with the stock culture? - Answers-1
We will grow microbe cultures at their optimal growth
temperatures for ~24 hours in the _______ - Answers-
incubator
Check all of the following "tools of the trade" that uses
heat to destroy microorganisms in the lab. - Answers-
autoclave and microincinerator
What would you do to determine if you have a
contaminated/mixed broth culture? - Answers-quadrant
streak plate
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How does the transfer from an E. coli broth culture to broth
media differ from the transfer from an E. coli broth culture
to slant media? - Answers-The loopful of culture is plunged
into the broth tube but spread over the surface of the slant
tube.
Why do we sterilize the loop before we sample the culture
we streak onto the first quadrant, and each time before
streaking subsequent quadrants in a streak plate? -
Answers-To decrease the amount of bacteria in
subsequent quadrants AND to prevent contamination of
your stock culture.
When inoculating a broth from a colony, how long should
you hold the loop in the heat of the microincinerator (or
Bunsen burner)? - Answers-until the loop turns red
Which microbe is a risk level-4 and requires biosafety level
4 (BSL-4) to work with it? - Answers-Ebola virus
WEEK TWO Mini-Exam - Answers-