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BIO411 CELL BIOLOGY LABORATORY REPORT LATEST VERSION

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BIO411 CELL BIOLOGY LABORATORY REPORT

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BIO411 CELL
BIOLOGY
LABORATORY REPORT


VIRTUAL DRY LAB 3:
FLUID MOVEMENT - OSMOSIS




Group Members 1. MUHAMMAD NABIL HAQIEM BIN HISHAM
(2020615298)
2. ANIS SURAYA BINTI AZMI (2020449854)
3. NUR AQILLAH NATASYA BT ABD GHAFAR
(2020605126)
Lecturer’s Name MADAM NURULHUDA BINTI ISMAIL
Group/Class AS201 1A1
Date of Experiment 18th NOVEMBER 2020

,1.0 INTRODUCTION

Osmosis can be defined as the phenomenon of selective transport of solvent
molecules across a semipermeable membrane from a dilute solution to a
more concentrated one [ CITATION Joh19 \l 17417 ]. It accounts for fluid
transport out of the kidney tubules and the gastrointestinal tract, into capillaries,
and across cell membranes. The semipermeable membrane enables the
movement of solvent molecules but prohibits the passage of solvent molecules.
Movement of water via cell membranes by osmosis is important for all
physiological processes. In general, tonicity and osmolarity of a solution play a
huge role in osmosis. Tonicity and osmolarity are properties of a solution,
independent of any membrane. [ CITATION Red16 \l 17417 ]. Tonicity is a
measure of effective osmolarity in cell biology whereas osmolarity is a
concentration measure that represents the overall concentration of solute
particles and is specifically related to either of the four colligative properties,
which are vapour pressure lowering, boiling point elevation, freezing point
depression, and osmotic pressure. Osmotic pressure is the pressure that drives
osmosis and is controlled by the number of particles of solute in a solution.

Tonicity is generally classified into three types, hypertonicity, isotonicity and
hypotonicity. An isotonic solution is a medium in which the overall
concentration of solutes is found inside a cell. It means that there is no net
change or water leaves and enters the cell at the same rate. When this happens,
the solutions are often called as in equilibrium. A hypotonic solution is a
medium in which its concentration of solutes is lower than that of the inside of
the cell. In other words, the amount of water enters the cell overwhelms the
amount of water that leaves the cell. On the other hand, a hypertonic solution is
a medium in which its concentration of solutes is higher than that of inside the
cell. To put it simply, the amount of water that enters the cells is significantly
lower than that water leaves the cell (Tortora, Funke & Case, 2016). Both
hypertonic and hypotonic conditions can deal with significant effects on the cell
if either one occurs. For instance, a cell in a hypotonic solution will have water
molecules overwhelmingly entering the cell. If the cell has a strong cell wall, it

, will contain the swelling. However, if the cell wall is weak, the cell will burst
and rupture, which this condition of the cell is called osmotic lysis. On the other
hand, a cell in a hypertonic solution will have water molecules excessively leave
the cell. This will cause its cytoplasm to shrink and collapse. This condition of
the cell is called plasmolysis.

In this experiment, most of the materials and apparatus are household items.
One of the reasons for using household items is that we already have these items
making it more accessible. Other than that, the cost to do the entire experiment
will be cheaper. In this experiment, we recognise the experimental parameter by
recording the initial and final in potato strips length (cm) and calculate the
average changes in length (%). Finally, we will

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BIO411 CELL BIOLOGY LABORATORY REPORT

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