UF DNA & Serology Final Exam Questions with Correct Answers Latest Update 2026/2027
______ is credited with the first practical forensic application of human DNA analysis. - Answers
Alec Jeffreys
RFLP is ____ sensitive as PCR methods. - Answers less
Which method uses the smallest fragments of DNA? - Answers STR
CODIS looks for relationships between the profiles of unidentified remains and relatives of
missing persons. - Answers True
short tandem repeats (STRs) are also known as _____ - Answers Microsatellites
Which is not in DAB standard 6? - Answers D. Amplified DNA does not need to be in separate
area than DNA extraction and PCR set-up.
When pipetting the pipet tip should be placed - Answers at the top of the liquid sample
If you are LEFT handed, the order of movement would be as follows: - Answers Left to right
Which is NOT appropriate during sample preparation? - Answers Process both question
samples and reference samples together.
What is NOT considered a type of eye protection? - Answers Contacts
Which extraction method is the most appropriate for a tissue sample? - Answers Organic
Which extraction method is possibly too harsh for a very old, degraded bloodstain? - Answers
Chelex
Which extraction method is the most appropriate for a vaginal swab from a sexual assault kit? -
Answers Differential
Which extraction method is the most appropriate for a buccal swab (reference standard)? -
Answers Either Chelex or Organic
What is the main type of buffer used in the organic extraction? - Answers Digest Buffer
What is the purpose of the phenol in the phenol:chloroform organic extraction? - Answers
Solubalize polysaccharides
The Chelex resin is selective for______? - Answers Heavy metals and cations
Hairs that have been shed from the head (naturally fallen) are of what category? - Answers
Telogen
What color blood tubes are preferred to use if DNA extraction is necessary? - Answers Purple
, Generally chelex extraction is used in the recovery of DNA from oral samples. However, due to
the nature of one type of sample organic extraction may be considered to provide better or
more reliable results. Which sample type is it? - Answers Chewing gum
True or False: The yield gel only measures total human DNA. - Answers False
Nucleic acids have UVVis absorption characteristics at which wavelength range? - Answers 240-
280 nm
True or False The slot blot method of DNA quantitation can measure both double and single-
stranded DNA? - Answers True
RT-PCR utilizes a modification of what? - Answers Polymerase Chain Reaction
What type of equipment is necessary to use for RT-PCR? - Answers Thermal cycler
True or False? Multiplex PCR uses more than one set of PCR primers to target multiple
locations. - Answers True
True or False The target amount of extracted DNA necessary for PCR is in the region of 0.5 2 µg.
- Answers False
The short polynucleotide chains used in PCR to target regions of amplification are called? -
Answers Primers
In what organism is Taq Polymerase found? - Answers Thermus Aquaticus
What is the purpose of EDTA during the PCR reaction? - Answers chelator
DNA fragments will travel - Answers Toward the anode and away from the cathode
How many STR loci can you get information about using Profiler and Cofiler kits? - Answers 13
By using the Profiler and Cofiler Kits how
many amplifications does it take to obtain information on all the CODIS loci? - Answers 2
Typical data applied to an electropherogram does NOT include - Answers Peak Area
But it does include: name of locus, number of repeats, size of fragment, and peak height.
What does the genescan software NOT do? - Answers Assigns allele calls to the peaks
Assuming two donors, consider one locus with alleles 12, 13 and 14. How many different
ALLELIC combinations are possible for one of the individuals in the mixture? - Answers 6
True or False: You can analyze your data without an allelic ladder? - Answers False
______ is credited with the first practical forensic application of human DNA analysis. - Answers
Alec Jeffreys
RFLP is ____ sensitive as PCR methods. - Answers less
Which method uses the smallest fragments of DNA? - Answers STR
CODIS looks for relationships between the profiles of unidentified remains and relatives of
missing persons. - Answers True
short tandem repeats (STRs) are also known as _____ - Answers Microsatellites
Which is not in DAB standard 6? - Answers D. Amplified DNA does not need to be in separate
area than DNA extraction and PCR set-up.
When pipetting the pipet tip should be placed - Answers at the top of the liquid sample
If you are LEFT handed, the order of movement would be as follows: - Answers Left to right
Which is NOT appropriate during sample preparation? - Answers Process both question
samples and reference samples together.
What is NOT considered a type of eye protection? - Answers Contacts
Which extraction method is the most appropriate for a tissue sample? - Answers Organic
Which extraction method is possibly too harsh for a very old, degraded bloodstain? - Answers
Chelex
Which extraction method is the most appropriate for a vaginal swab from a sexual assault kit? -
Answers Differential
Which extraction method is the most appropriate for a buccal swab (reference standard)? -
Answers Either Chelex or Organic
What is the main type of buffer used in the organic extraction? - Answers Digest Buffer
What is the purpose of the phenol in the phenol:chloroform organic extraction? - Answers
Solubalize polysaccharides
The Chelex resin is selective for______? - Answers Heavy metals and cations
Hairs that have been shed from the head (naturally fallen) are of what category? - Answers
Telogen
What color blood tubes are preferred to use if DNA extraction is necessary? - Answers Purple
, Generally chelex extraction is used in the recovery of DNA from oral samples. However, due to
the nature of one type of sample organic extraction may be considered to provide better or
more reliable results. Which sample type is it? - Answers Chewing gum
True or False: The yield gel only measures total human DNA. - Answers False
Nucleic acids have UVVis absorption characteristics at which wavelength range? - Answers 240-
280 nm
True or False The slot blot method of DNA quantitation can measure both double and single-
stranded DNA? - Answers True
RT-PCR utilizes a modification of what? - Answers Polymerase Chain Reaction
What type of equipment is necessary to use for RT-PCR? - Answers Thermal cycler
True or False? Multiplex PCR uses more than one set of PCR primers to target multiple
locations. - Answers True
True or False The target amount of extracted DNA necessary for PCR is in the region of 0.5 2 µg.
- Answers False
The short polynucleotide chains used in PCR to target regions of amplification are called? -
Answers Primers
In what organism is Taq Polymerase found? - Answers Thermus Aquaticus
What is the purpose of EDTA during the PCR reaction? - Answers chelator
DNA fragments will travel - Answers Toward the anode and away from the cathode
How many STR loci can you get information about using Profiler and Cofiler kits? - Answers 13
By using the Profiler and Cofiler Kits how
many amplifications does it take to obtain information on all the CODIS loci? - Answers 2
Typical data applied to an electropherogram does NOT include - Answers Peak Area
But it does include: name of locus, number of repeats, size of fragment, and peak height.
What does the genescan software NOT do? - Answers Assigns allele calls to the peaks
Assuming two donors, consider one locus with alleles 12, 13 and 14. How many different
ALLELIC combinations are possible for one of the individuals in the mixture? - Answers 6
True or False: You can analyze your data without an allelic ladder? - Answers False
