BIOL 351/ BIOL351 Final Exam
V2: (Latest 2026/ 2027 Update)
100 out of 100 | Questions &
correct Answers | Grade A
A promoter is required for the initiation of which of the following processes?
Transcription
Why is the initially transcribed RNA precursor much larger than the mature mRNA?
The initial transcript contains introns, which are removed by spilcing
Which of the following is the most accurate statement about enhancers?
Transcription factors bind to enhancers
Which of the following techniques would allow you to visualize gene expression in a living
zebra fish embryo, thus permitting repeated observations over time?
Fluorescent transgenic reporter
A transgene was constructed that mutated the MEF-2 site, but not the E1 site. The mutant MEF-2
site transgene was expressed in all muscles except limbs. What would you conclude about the
MEF-2 site?
It directs expression of the transgene in the limb muscle only
, BIOL 351/ BIOL351 Final Exam
V2: (Latest 2026/ 2027 Update)
100 out of 100 | Questions &
correct Answers | Grade A
If the MEF-2 site directs expression of the LacZ gene in certain skeletal muscles, what must it
bind to?
Transcription factors
A transgenic mouse is not a chimera, why?
Electroporation of vector DNA into ES cells results in an embryo in which all cells have the
transgene
What will be the result of thymidine kinase gene (TK) being incorporated into an ES cell's
genome?
It will make cells sensitive to Glancyclovir
You want to knockout the Zombie gene in the brain only. What will allow you to do this
experiment?
A mouse with a Cre recombinase that is expressed in the brain and a mouse with a floxed
Zombie allele
, BIOL 351/ BIOL351 Final Exam
V2: (Latest 2026/ 2027 Update)
100 out of 100 | Questions &
correct Answers | Grade A
You have ES cells that have passed through double selection for your knockout experiment.
What is your next step?
Inject the ES cells into a blastocyst
During the period of limb bud outgrowth, a developmental biologist has transplanted some
mesenchyme from underneath the AER of a forelimb bud an moved it underneath the AER of the
hindlimb bud. What would you expect from this surgically altered limb bud?
An appendage that is proximal leg and distal wing
The__ is the morphogen expressed in the __ that caudalizes the limb bud
SHH, zone of polarizing activity
Which of the following terms describes the step at which cells are committed to a particular cell
fate, but it is still reversible?
Specification
A parasitic infection of the frog embryo has resulted in the following defect. The parasites
induced expression of __ and the formation of extra__. (Photo shows frog will multiple hind
limbs)
FGF8, apical ectodermal ridge
, BIOL 351/ BIOL351 Final Exam
V2: (Latest 2026/ 2027 Update)
100 out of 100 | Questions &
correct Answers | Grade A
Scientists have created a transgenic chick embryo that expresses Tbx5 in both the forelimb and
hindlimb bud regions but no Tbx4. What would you expect to see?
A chicken with 4 legs
EMS cell normally divides and gives rise to cells that will form the mesoderm and endoderm. If
killing the P2 cells leads to the disruption of differentiation, which of the following best
describes the process taking place?
Conditional specification of the EMS cell
The nematode Caenorhabditis elegans is an outstanding model for developmental genetics due to
several features. Which of the following is not a feature of this nematode?
Every cell in this nematode undergoes autonomous specification
Spermatozoa that are harvested from the epididymis for IVF in men with blockage of their vas
deferens are incompetent to fertilize eggs. What must first happen for successful fertilization?
Capacitation
In sea urchins, the acrosome reaction:
V2: (Latest 2026/ 2027 Update)
100 out of 100 | Questions &
correct Answers | Grade A
A promoter is required for the initiation of which of the following processes?
Transcription
Why is the initially transcribed RNA precursor much larger than the mature mRNA?
The initial transcript contains introns, which are removed by spilcing
Which of the following is the most accurate statement about enhancers?
Transcription factors bind to enhancers
Which of the following techniques would allow you to visualize gene expression in a living
zebra fish embryo, thus permitting repeated observations over time?
Fluorescent transgenic reporter
A transgene was constructed that mutated the MEF-2 site, but not the E1 site. The mutant MEF-2
site transgene was expressed in all muscles except limbs. What would you conclude about the
MEF-2 site?
It directs expression of the transgene in the limb muscle only
, BIOL 351/ BIOL351 Final Exam
V2: (Latest 2026/ 2027 Update)
100 out of 100 | Questions &
correct Answers | Grade A
If the MEF-2 site directs expression of the LacZ gene in certain skeletal muscles, what must it
bind to?
Transcription factors
A transgenic mouse is not a chimera, why?
Electroporation of vector DNA into ES cells results in an embryo in which all cells have the
transgene
What will be the result of thymidine kinase gene (TK) being incorporated into an ES cell's
genome?
It will make cells sensitive to Glancyclovir
You want to knockout the Zombie gene in the brain only. What will allow you to do this
experiment?
A mouse with a Cre recombinase that is expressed in the brain and a mouse with a floxed
Zombie allele
, BIOL 351/ BIOL351 Final Exam
V2: (Latest 2026/ 2027 Update)
100 out of 100 | Questions &
correct Answers | Grade A
You have ES cells that have passed through double selection for your knockout experiment.
What is your next step?
Inject the ES cells into a blastocyst
During the period of limb bud outgrowth, a developmental biologist has transplanted some
mesenchyme from underneath the AER of a forelimb bud an moved it underneath the AER of the
hindlimb bud. What would you expect from this surgically altered limb bud?
An appendage that is proximal leg and distal wing
The__ is the morphogen expressed in the __ that caudalizes the limb bud
SHH, zone of polarizing activity
Which of the following terms describes the step at which cells are committed to a particular cell
fate, but it is still reversible?
Specification
A parasitic infection of the frog embryo has resulted in the following defect. The parasites
induced expression of __ and the formation of extra__. (Photo shows frog will multiple hind
limbs)
FGF8, apical ectodermal ridge
, BIOL 351/ BIOL351 Final Exam
V2: (Latest 2026/ 2027 Update)
100 out of 100 | Questions &
correct Answers | Grade A
Scientists have created a transgenic chick embryo that expresses Tbx5 in both the forelimb and
hindlimb bud regions but no Tbx4. What would you expect to see?
A chicken with 4 legs
EMS cell normally divides and gives rise to cells that will form the mesoderm and endoderm. If
killing the P2 cells leads to the disruption of differentiation, which of the following best
describes the process taking place?
Conditional specification of the EMS cell
The nematode Caenorhabditis elegans is an outstanding model for developmental genetics due to
several features. Which of the following is not a feature of this nematode?
Every cell in this nematode undergoes autonomous specification
Spermatozoa that are harvested from the epididymis for IVF in men with blockage of their vas
deferens are incompetent to fertilize eggs. What must first happen for successful fertilization?
Capacitation
In sea urchins, the acrosome reaction:
