BMS 525 EXAM 1 QUESTIONS AND ANSWERS GRADED A+ 2025/2026

BMS 525 EXAM 1 QUESTIONS AND
ANSWERS GRADED A+ 2025/2026




The study of Gene expression - ANS Molecular Biology Def


Nuclease - ANS enzyme that degrades nucleic acids via hydrolyzing and ester bond


Tm - ANS Temperature at which 50% of the DNA strand is denatured


Agarose Gel Electrophoresis - ANS separates DNA molecules by size


-EtBr for staining


Blotting steps - ANS 1. isolate DNA (restriction enzymes)


2. Run electrophoresis


3. Transfer to blot


4. Hybridize with DNA probe


PCR steps - ANS 1. Denaturation 30s
2. Annealing 30s

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, 3. Extension 30s then 5 mins


Run product on agarose gel and stain with EtBr


PCR requires (5) - ANS -Template DNA
-Primers
-dNTPs
-Taq polymerase
-bugger (Mg2+)


RT-PCR - ANS Uses Reverse transcriptase to create cDNA for PCR.


-measures RNA expression levels


qPCR (Real-time) - ANS Detects PCR products in real time


-Probes that fluoresce is used for detection


-detection during exponential phase of PCR


-Optical module detects fluorescence and computer quantifies the data


qPCR probes/dyes - ANS TaqMan - 5' reporter is cleaved off for fluorescence


SYBR green Dye - binds to dsDNA and fluoresces


Structure of DNA polymer - ANS



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