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DNA Fingerprinting

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, DNA FINGERPRINTING


DNA fingerprinting, in genetics , method
of isolating and identifying v a ria b le
elements within the base-pair sequ e nce of
DNA (deoxyribonucleic acid). T he
technique was developed in 198 4 by
British geneticist Alec Jeffreys , a fte r he
noticed that certain sequences of h igh l y
variable DNA (known as minisatellites ),
which do not contribute to the fun c tio n s
of genes , are repeated within genes.
Jeffreys recognized that each i ndi v idu a l
has a unique pattern of minisatellites (the
only exceptions being m ult i ple
individuals from a single zygote , such as
identical twins).
In simple words it is a la b ora t ory
technique/chemical test that s h ows
genetic makeup of a person.

PRINCIPLE -

It involves identifying difference s in
some specific regions in D N A
sequence called as Repetitive DNA.

The combination of DNA sequ enc e s t h at f req u ent l y d i ffe r sig nif icant ly
between people is identified by DN A fi n ger p rin t ing or D NA p rof iling .
Short nucleotide repeats that are in h eri t ed an d v ary i n n umb e r fro m pe rson
to person are the most crucial nee d fo r DN A fi n ger p rin t ing . Th ese a re
referred to as VNTRs or variable number tandem repea ts.
The DNA fingerprinting method i s bas e d on th e i dea t h at no b ody h as an
identical DNA sequence, except for ide n tic a l t w ins (mo n ozy g oti c twi ns) .

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