► RESULTS for Exercise 10
Table 10-1: Measurern
ent of spots on filter paper using a P20 and a P200
Volume spotted Spot] Spor 2 Spot3
P20
5 µI
C), '1 L,O 0. °'
CCM Cwi
CVV?
10 µI
,o I I• I ,{\ ). <
I
r-
1
Io \
C-W'l CVI/\
cvv-i
20 µI
I 1) '2- l. b (" b
C VV'\ CW\ CVY1
P200 20 µI ,z. 0\
2 .f, 2., 0
C,1.1i1
OVV\ cVvi
50 µI "2.. q -z: .. 9J
'1-~ ~
C,W1 CVv\
G'-\/\
Table 10-2: Measurement of cells using a Pl000 pipettor.
2
Tube Dilution 1 Absorban ce 640 nm Total Absorba nce
0, "2 O ,Ort
0.2
5-=: Q.O'-f Oo0'1 -
0-0t-t -- \. '15
D .t5
0.5
0.5
.,.__-
!5 -- 0- \ Oo \'5 - 0. l - \ 0 'S
1 -s
I
~
0 .. 1- 0~1- 6
0.'2-t
- z --- lo~
1 To calculate the dilution factor, remember:
Dilution =volume of sample/(volume of sample + volume of diluent)
2 Total absorbance =Absorbance at 640 nm/ by dilution calculated earlier
Eurdst10 Use of a Miaopipettor 111
Table 10-1: Measurern
ent of spots on filter paper using a P20 and a P200
Volume spotted Spot] Spor 2 Spot3
P20
5 µI
C), '1 L,O 0. °'
CCM Cwi
CVV?
10 µI
,o I I• I ,{\ ). <
I
r-
1
Io \
C-W'l CVI/\
cvv-i
20 µI
I 1) '2- l. b (" b
C VV'\ CW\ CVY1
P200 20 µI ,z. 0\
2 .f, 2., 0
C,1.1i1
OVV\ cVvi
50 µI "2.. q -z: .. 9J
'1-~ ~
C,W1 CVv\
G'-\/\
Table 10-2: Measurement of cells using a Pl000 pipettor.
2
Tube Dilution 1 Absorban ce 640 nm Total Absorba nce
0, "2 O ,Ort
0.2
5-=: Q.O'-f Oo0'1 -
0-0t-t -- \. '15
D .t5
0.5
0.5
.,.__-
!5 -- 0- \ Oo \'5 - 0. l - \ 0 'S
1 -s
I
~
0 .. 1- 0~1- 6
0.'2-t
- z --- lo~
1 To calculate the dilution factor, remember:
Dilution =volume of sample/(volume of sample + volume of diluent)
2 Total absorbance =Absorbance at 640 nm/ by dilution calculated earlier
Eurdst10 Use of a Miaopipettor 111
