ASCP MB Review EXAM, Most Recent Exam 2026
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What specimen has the most DNA according to molecular
weight? - Answers-White Blood Cells
What amino acids compose histones? - Answers-Lysine
and Arginine
Amino acid that all proteins start with: - Answers-
Methionine
What protects the DNA strand during replication? -
Answers-Single-stranded binding protein
Leading Strand - Answers-3' to 5' template
Lagging Strand - Answers-5' to 3' template
,2|Page
What DNA strand is being copied in transcription? -
Answers-Antisense Strand
First step after newly formed mRNA is made? - Answers-
Addition of poly(A) tail
Every time the chromosome is copied it would become
shorter if not for what enzyme? - Answers-Telomerase
What polymerase has repair activity? - Answers-
Beta/Epsilon
What polymerase acts on leading strand? - Answers-Delta
What polymerase acts on lagging strand? - Answers-Alpha
Which polymerase is used for mitochondrial replication
and repair? - Answers-Gamma
What polymerase binds the 5' end to 3' end of dsDNA? -
Answers-Topoisomerase
,3|Page
Which RNA polymerase is mainly for transcription? -
Answers-RNA Pol II
Which polymerase is involved with mRNA synthesis? -
Answers-RNA Pol II
What inactivates RNAses? - Answers-Diethyl
pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours
at 400C
What type of restriction enzyme recognizes palindromic
sites? - Answers-Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity?
- Answers-Restriction Enzyme Type II
What is necessary to make cDNA? - Answers-Reverse
transcriptase
Predominant form of DNA - Answers-Right-handed B
DNA and histone interaction is due to what type of bond? -
Answers-Ionic
, 4|Page
What stains to the minor groove of DNA duplex? -
Answers-SYBR Green
What is the most abundant type of RNA? - Answers-
Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - Answers-Genome
and mRNA
What increases half-life of mRNA? - Answers-5' Methyl
Cap
What kind of DNA is most likely to be transcriptionally
active? - Answers-Euchromatin
What is the most common type of eukaryotic DNA? -
Answers-Satellite DNA
Number of SNPs in a human genome? - Answers-11
billion (10^7)