ASCP Molecular Biology Certification Exam Verified
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Find the palindrome in this restriction enzyme site: 5'-
CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - Answers-3'-GAC
A patient with impaired judgment, personality changes,
signs of abnormal body movements and depression
comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the
cause of those clinical manifestations. A blood specimen
was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard
PCR indicates that patient has Huntington Disease, HD.
Which of the following would be consistent with this
diagnosis?
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25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - Answers-85
CAG repeats in the Huntington gene
Which two HPV types are responsible for most cases of
cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - Answers-16 and 18
Replication forks, known as origins of DNA replication, are
created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase - Answers-Helicase
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Mutation in what gene is associated with Fragile X
syndrome? - Answers-FMR1
Mantle cell lymphoma (MCL) is caused by what
translocation? - Answers-t(11;14)
This polymerase is involved in "initiation of DNA replication
and has primase activity": - Answers-Pol α
Its discovery shed light on why there is simultaneous,
though not continuous, synthesis of DNA on both leading
and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments - Answers-Okazaki fragments
What gene is measured following treatment with imatinib
(Gleevec)?
FLT3
BCR/Abl
Jak2
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MAPK - Answers-BCR/Abl
What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second - Answers-50 nucleotides per
second
This polymerase is involved in "replicates mitochondrial
DNA": - Answers-Pol γ
A patient with impaired judgment, personality changes,
signs of abnormal body movements and depression
comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the
cause of those clinical manifestations. A blood specimen
was then sent to your clinical laboratory for mutation
screening in the Huntingtin gene. Which of these methods
would best accomplish this task?
Methylation-specific PCR
Standard PCR