BIOL 3150 Final Exam Questions and
All Correct Answers.
When is the best time to collect a specimen for culture? - Answer At the time of a
temperature evaluation.
Sterile containers - Answer Containers free of any and all bacteria and microorganisms.
Nonsterile containers - Answer Containers not free of any and all bacteria and
microorganisms.
What information is needed when labeling a specimen? - Answer At least two patient
identifiers (name, birthdate, social security number, hospital ID, etc.), time and date of
collection, and collector's name and signature.
What is transport media and why is it important? - Answer Solutions that preserve the
viability of specimens during transport.
Most common source of contamination and how contamination usually come about in these
samples in a hospital setting. - Answer Nosocomial infections are caused by healthcare works
touching medical objects without gloves or washing hands.
BAP media - Answer Tests for the ability of an organism to produce hemolysins (enzymes
that damage red blood cells).
MAC media - Answer Only grows gram-negative bacteria.
Indole test - Answer Tests for the ability of microorganisms to degrade the amino acid
tryptophan. Negative results turn red and positive results turn blue.
TSI test - Answer Tests for the ability for a microorganism to ferment sugars and produce
hydrogen sulfide. An alkaline/alkaline (red slant, red butt) indicates the absence of carbohydrate
fermentation results. An alkaline/acid (red slant, yellow butt) reaction indicates it is indicative of
dextrose fermentation only. An acid/acid (yellow slant, yellow butt) reaction indicates the
fermentation of dextrose, lactose and/or sucrose. Blacking of the media indicates the presence
of H2. Bubbles or cracks in the agar indicates the production of gas.
Coagulase test - Answer Tests for the difference between Staphylococci aureus and other
Staphylococci species such as Staphylococci epidermidis.
All Correct Answers.
When is the best time to collect a specimen for culture? - Answer At the time of a
temperature evaluation.
Sterile containers - Answer Containers free of any and all bacteria and microorganisms.
Nonsterile containers - Answer Containers not free of any and all bacteria and
microorganisms.
What information is needed when labeling a specimen? - Answer At least two patient
identifiers (name, birthdate, social security number, hospital ID, etc.), time and date of
collection, and collector's name and signature.
What is transport media and why is it important? - Answer Solutions that preserve the
viability of specimens during transport.
Most common source of contamination and how contamination usually come about in these
samples in a hospital setting. - Answer Nosocomial infections are caused by healthcare works
touching medical objects without gloves or washing hands.
BAP media - Answer Tests for the ability of an organism to produce hemolysins (enzymes
that damage red blood cells).
MAC media - Answer Only grows gram-negative bacteria.
Indole test - Answer Tests for the ability of microorganisms to degrade the amino acid
tryptophan. Negative results turn red and positive results turn blue.
TSI test - Answer Tests for the ability for a microorganism to ferment sugars and produce
hydrogen sulfide. An alkaline/alkaline (red slant, red butt) indicates the absence of carbohydrate
fermentation results. An alkaline/acid (red slant, yellow butt) reaction indicates it is indicative of
dextrose fermentation only. An acid/acid (yellow slant, yellow butt) reaction indicates the
fermentation of dextrose, lactose and/or sucrose. Blacking of the media indicates the presence
of H2. Bubbles or cracks in the agar indicates the production of gas.
Coagulase test - Answer Tests for the difference between Staphylococci aureus and other
Staphylococci species such as Staphylococci epidermidis.
