BIOL 3150 LAB 2- FINAL EXAM
QUESTIONS AND ALL CORRECT
ANSWERS.
Cytochrome C oxidase - Answer the last enzyme in the respiratory electron transport chain
of bacteria
What does cytochrome C oxidase do? - Answer receives an electron from each four
cytochrome molecules. Then transfers them to one oxygen molecule
how to perform oxidase test - Answer streak a small portion of colony and soak it with the
reagent. if the streak turns purple within 30 seconds it is positive
I- represents what in the IMVic Tests - Answer tests for Indol from tryptophan
M represents what in the IMViC tests - Answer Methyl red. and to test for stable production
from glucose
V represents what in the IMViC tests - Answer Voges-Proskauer test for production of
acetoin from glucose
C represents what in the IMViC tests - Answer tests for citrate as the sole carbon source
Methyl Red test distinguishes what? - Answer the ability to catabolize glucose into stable
mixed acids
Results for MR-VP test - Answer Red= (+) for mixed acid formation
Yellow= (-) for mixed acid formation
Voges-Proskauer test distinguishes what? - Answer the ability to catabolize glucose into
neutral end products
Results from VP test - Answer Red= positive for acetoin
normal copper color= no acetoin
QUESTIONS AND ALL CORRECT
ANSWERS.
Cytochrome C oxidase - Answer the last enzyme in the respiratory electron transport chain
of bacteria
What does cytochrome C oxidase do? - Answer receives an electron from each four
cytochrome molecules. Then transfers them to one oxygen molecule
how to perform oxidase test - Answer streak a small portion of colony and soak it with the
reagent. if the streak turns purple within 30 seconds it is positive
I- represents what in the IMVic Tests - Answer tests for Indol from tryptophan
M represents what in the IMViC tests - Answer Methyl red. and to test for stable production
from glucose
V represents what in the IMViC tests - Answer Voges-Proskauer test for production of
acetoin from glucose
C represents what in the IMViC tests - Answer tests for citrate as the sole carbon source
Methyl Red test distinguishes what? - Answer the ability to catabolize glucose into stable
mixed acids
Results for MR-VP test - Answer Red= (+) for mixed acid formation
Yellow= (-) for mixed acid formation
Voges-Proskauer test distinguishes what? - Answer the ability to catabolize glucose into
neutral end products
Results from VP test - Answer Red= positive for acetoin
normal copper color= no acetoin
