Liquid Chromatography Mass spectrometry (LCMS)
SUBMITTED
TO
THE DEPARTMENT OF CHEMISTRY
SAVITRIBAI PHULE PUNE UNIVERSITY
Written by:
Rutuja Gajanan Deosarkar
Under the guidance of:
Dr. Ayesha Khan
Department of chemistry, Savitribai Phule Pune Univeraity
20 September, 2025
, 1. Introduction
Definition: LC‑MS is a hyphenated analytical technique
combining a liquid chromatography separation stage
followed by mass spectrometric detection. The LC separates
complex mixtures into individual analytes; MS detects,
characterizes, and quantifies them based on their
mass‑to‑charge (m/z) ratio.
Why use LC‑MS: Many compounds are nonvolatile,
thermally labile, or polar, making gas chromatography hard
or impossible; LC handles these well. MS provides high
sensitivity, specificity, and structural information (via
fragmentation).
Scope: Used in pharmaceuticals, proteomics, metabolomics,
environmental, food safety, forensics, clinical diagnostics,
etc.
Historical context & growth: Development of robust
interfaces (e.g. electrospray) and tandem MS (MS/MS) have
driven wide adoption in pharmaceuticals, bioanalysis,
metabolomics, proteomics, food safety, environment, etc.
SUBMITTED
TO
THE DEPARTMENT OF CHEMISTRY
SAVITRIBAI PHULE PUNE UNIVERSITY
Written by:
Rutuja Gajanan Deosarkar
Under the guidance of:
Dr. Ayesha Khan
Department of chemistry, Savitribai Phule Pune Univeraity
20 September, 2025
, 1. Introduction
Definition: LC‑MS is a hyphenated analytical technique
combining a liquid chromatography separation stage
followed by mass spectrometric detection. The LC separates
complex mixtures into individual analytes; MS detects,
characterizes, and quantifies them based on their
mass‑to‑charge (m/z) ratio.
Why use LC‑MS: Many compounds are nonvolatile,
thermally labile, or polar, making gas chromatography hard
or impossible; LC handles these well. MS provides high
sensitivity, specificity, and structural information (via
fragmentation).
Scope: Used in pharmaceuticals, proteomics, metabolomics,
environmental, food safety, forensics, clinical diagnostics,
etc.
Historical context & growth: Development of robust
interfaces (e.g. electrospray) and tandem MS (MS/MS) have
driven wide adoption in pharmaceuticals, bioanalysis,
metabolomics, proteomics, food safety, environment, etc.
