MILT 525 EXAM 2 COMPREHENSIVE TEST 2026
QUESTIONS WITH ANSWERS GRADED A+
◉ Meselson-Stahl Experiment
Research question?
Expected Results?
Conclusion?. Answer: Goal: which type of DNA replication occurs?
Results Conservative:
-after one generation, one heavy band and one light band
-after two generations, one heavy and one light
Results Semiconservative:
-after one generation, one intermediate hybrid band
-after two generations, one light band and one hybrid band
Conclusion:
DNA replication is semiconservative because hybrids
◉ Meselson-Stahl Experiment
Set up. Answer: Grew cells in heavy N and then in light N
,-DNA was then isolated and put into a gradient followed by a
radiograph
◉ Griffith's experiment. Answer: Something in the heat-killed S
strain was able to enter into the R strain and make it virulent
◉ Avery, MacLeod, and McCarty experiment. Answer: Took heat-
killed S strains and added it to tubes lacking either DNA, RNA, or
protein
-in the tube lacking DNA, no transformation occurred and the mouse
survived
-proved DNA is hereditary material
◉ Hershy-Chase Experiment. Answer: Bacteriophage test
-DNA = P32
-Protein = S35
DNA was found in the cell after infection
-Discovered DNA is required for viral replication
, ◉ Products of a gene. Answer: -RNA that may or may not encode
polypeptides
-A sequence of DNA that specifies the sequence of an independent
product
◉ one gene-one enzyme hypothesis. Answer: -a gene is responsible
for the production of a single enzyme
◉ one gene-one enzyme hypothesis PROBLEMS. Answer: -not all
genes make protein
-not all proteins are enzymes
-one gene can make more than one enzyme
-a single gene can code for different types of proteins
-an enzyme can be made up of multiple polyproteins
-not all enzymes are made from one gene
QUESTIONS WITH ANSWERS GRADED A+
◉ Meselson-Stahl Experiment
Research question?
Expected Results?
Conclusion?. Answer: Goal: which type of DNA replication occurs?
Results Conservative:
-after one generation, one heavy band and one light band
-after two generations, one heavy and one light
Results Semiconservative:
-after one generation, one intermediate hybrid band
-after two generations, one light band and one hybrid band
Conclusion:
DNA replication is semiconservative because hybrids
◉ Meselson-Stahl Experiment
Set up. Answer: Grew cells in heavy N and then in light N
,-DNA was then isolated and put into a gradient followed by a
radiograph
◉ Griffith's experiment. Answer: Something in the heat-killed S
strain was able to enter into the R strain and make it virulent
◉ Avery, MacLeod, and McCarty experiment. Answer: Took heat-
killed S strains and added it to tubes lacking either DNA, RNA, or
protein
-in the tube lacking DNA, no transformation occurred and the mouse
survived
-proved DNA is hereditary material
◉ Hershy-Chase Experiment. Answer: Bacteriophage test
-DNA = P32
-Protein = S35
DNA was found in the cell after infection
-Discovered DNA is required for viral replication
, ◉ Products of a gene. Answer: -RNA that may or may not encode
polypeptides
-A sequence of DNA that specifies the sequence of an independent
product
◉ one gene-one enzyme hypothesis. Answer: -a gene is responsible
for the production of a single enzyme
◉ one gene-one enzyme hypothesis PROBLEMS. Answer: -not all
genes make protein
-not all proteins are enzymes
-one gene can make more than one enzyme
-a single gene can code for different types of proteins
-an enzyme can be made up of multiple polyproteins
-not all enzymes are made from one gene
