2
Clinical Chemistry I ASCP review (youlazy) questions with || || || || || || || ||
precise answers ||
Which of the following lamps provides a continuous spectrum of radiant energy in the
|| || || || || || || || || || || || || ||
visible, near IR, and near UV regions of the spectrum?
|| || || || || || || || ||
A. Tungsten-filament
||
B. Hydrogen
||
C. Deuterium
||
D. Mercury vapor - ✔✔A most common light source for photometry in the visible region.
|| || || || || || || || || || || || || || ||
continuous spectrum (360-800 nm). near IR and UV. || || || || || || || ||
Hydrogen and Deuterium (200-375 nm) || || || || ||
Mercury vapor- not a continuous spectrum, emitting radiation at specific wavelengths
|| || || || || || || || || ||
Which of the following isolates light
|| || || || ||
within a narrow region of the spectrum?
|| || || || || ||
A. Photomultiplier tube
|| ||
B. Monochromator
||
C. Photovoltaic cell
|| ||
D. Detector - ✔✔B Use for selecting a narrow band wavelengths from a continuous
|| || || || || || || || || || || || || ||
spectrum. Three kinds (prisms, filters, diffraction gratings.
|| || || || || ||
Which of the following is not descriptive of a photomultiplier tube?
|| || || || || || || || || ||
A. Emits electrons proportionally to
|| || || ||
initial light absorbed
|| ||
B. Must be shielded from stray light
|| || || || || ||
,2
C. Cannot be used with a chopper
|| || || || || ||
D. Amplifies the initial signal received - ✔✔C
|| || || || || || ||
PMT- responds to the radiant energy light it absorbs by emitting electrons in a proportional
|| || || || || || || || || || || || || || || ||
amount to the initial light absorbed. || || || || ||
Which of the following is false about
|| || || || || ||
a photomultiplier tube?
|| ||
A. Converts radiant energy (light)
|| || || ||
to electrical energy (current)
|| || ||
B. Amplifies the current significantly
|| || || ||
C. Has a very rapid response time
|| || || || || ||
D. Is composed of an iron plate and
|| || || || || || ||
a layer of selenium - ✔✔D is part of a barrier layer cell
|| || || || || || || || || || || ||
PMT- 1. light to electricity
|| || || ||
2. Amplification
||
5. Which type of photodetector employs a linear arrangement that allows it to respond to a
|| || || || || || || || || || || || || || || ||
specific wavelength resulting in complete UV/visible spectrum analysis?
|| || || || || || ||
A. Photomultiplier tube
|| ||
B. Phototube
||
C. Barrier layer cell
|| || ||
D. Photodiode array - ✔✔D
|| || || || ||
256-2048 arranged in a linear fashion || || || || ||
Respond to a specific wavelength that results in a continuous UV/ visible spectrum.
|| || || || || || || || || || || ||
When performing spectrophotometer
|| ||
,2
quality assurance checks, what is the
|| || || || ||
holmium oxide glass filter used to assess?
|| || || || || ||
A. Linearity
||
B. Stray light
|| ||
C. Absorbance accuracy
|| ||
D. Wavelength accuracy - ✔✔D
|| || || ||
In spectrophotometric analysis, what is the purpose of the reagent blank?
|| || || || || || || || || ||
A. Correct for interfering chromogens
|| || || ||
B. Correct for lipemia
|| || ||
C. Correct for protein
|| || ||
D. Correct for color contribution of the reagents - ✔✔D
|| || || || || || || || ||
In regard to bichromatic analysis, which of the following is false?
|| || || || || || || || || ||
A. Absorbance is measured at the
|| || || || ||
spectral absorbance peak for a blank
|| || || || ||
and the sample using the same
|| || || || ||
wavelength.
B. Eliminates background interferences
|| || ||
C. Sample concentration determined
|| || ||
from difference in two measured
|| || || ||
absorbances
D. Functions as a reference blank for
|| || || || || ||
each sample - ✔✔A
|| || ||
The bandpass of a spectrophotometer is
|| || || || ||
, 2
10 nm. If an instalment is set at 540 nm, the wavelengths that are permitted to impinge on
|| || || || || || || || || || || || || || || || || ||
the sample will be within what
|| || || || ||
wavelength range? ||
A. 530-540 nm
|| ||
B. 530-550 nm
|| ||
C. 535-545 nm
|| ||
D. 540-550 nm - ✔✔C
|| || || || ||
540 +/- 5nm (10nm) || || ||
Which of the following formulas is an || || || || || ||
expression of the Beer-Lambert law that is || || || || || ||
routinely applied to spectrophotometric || || ||
analysis?
A. A u X C s/As = C u
|| || || || || || || ||
B. C u X C s/As= A u
|| || || || || || ||
C. A s X C s/C u = A u
|| || || || || || || || ||
D. A = 2 - log %T - ✔✔A
|| || || || || || || ||
1 1 . In spectrophotometry, which of the following is a mathematical expression of the
|| || || || || || || || || || || || || || ||
relationship between absorbance and transmittance? || || || ||
A. A — abc || || ||
R A u/ C u = A s/C s
|| || || || || || || ||
C. A = 2 - log %T
|| || || || || ||
D. A = log %T - ✔✔C
|| || || || || ||
Which of the following is not a problem inherent in turbidimetry?
|| || || || || || || || || ||
Clinical Chemistry I ASCP review (youlazy) questions with || || || || || || || ||
precise answers ||
Which of the following lamps provides a continuous spectrum of radiant energy in the
|| || || || || || || || || || || || || ||
visible, near IR, and near UV regions of the spectrum?
|| || || || || || || || ||
A. Tungsten-filament
||
B. Hydrogen
||
C. Deuterium
||
D. Mercury vapor - ✔✔A most common light source for photometry in the visible region.
|| || || || || || || || || || || || || || ||
continuous spectrum (360-800 nm). near IR and UV. || || || || || || || ||
Hydrogen and Deuterium (200-375 nm) || || || || ||
Mercury vapor- not a continuous spectrum, emitting radiation at specific wavelengths
|| || || || || || || || || ||
Which of the following isolates light
|| || || || ||
within a narrow region of the spectrum?
|| || || || || ||
A. Photomultiplier tube
|| ||
B. Monochromator
||
C. Photovoltaic cell
|| ||
D. Detector - ✔✔B Use for selecting a narrow band wavelengths from a continuous
|| || || || || || || || || || || || || ||
spectrum. Three kinds (prisms, filters, diffraction gratings.
|| || || || || ||
Which of the following is not descriptive of a photomultiplier tube?
|| || || || || || || || || ||
A. Emits electrons proportionally to
|| || || ||
initial light absorbed
|| ||
B. Must be shielded from stray light
|| || || || || ||
,2
C. Cannot be used with a chopper
|| || || || || ||
D. Amplifies the initial signal received - ✔✔C
|| || || || || || ||
PMT- responds to the radiant energy light it absorbs by emitting electrons in a proportional
|| || || || || || || || || || || || || || || ||
amount to the initial light absorbed. || || || || ||
Which of the following is false about
|| || || || || ||
a photomultiplier tube?
|| ||
A. Converts radiant energy (light)
|| || || ||
to electrical energy (current)
|| || ||
B. Amplifies the current significantly
|| || || ||
C. Has a very rapid response time
|| || || || || ||
D. Is composed of an iron plate and
|| || || || || || ||
a layer of selenium - ✔✔D is part of a barrier layer cell
|| || || || || || || || || || || ||
PMT- 1. light to electricity
|| || || ||
2. Amplification
||
5. Which type of photodetector employs a linear arrangement that allows it to respond to a
|| || || || || || || || || || || || || || || ||
specific wavelength resulting in complete UV/visible spectrum analysis?
|| || || || || || ||
A. Photomultiplier tube
|| ||
B. Phototube
||
C. Barrier layer cell
|| || ||
D. Photodiode array - ✔✔D
|| || || || ||
256-2048 arranged in a linear fashion || || || || ||
Respond to a specific wavelength that results in a continuous UV/ visible spectrum.
|| || || || || || || || || || || ||
When performing spectrophotometer
|| ||
,2
quality assurance checks, what is the
|| || || || ||
holmium oxide glass filter used to assess?
|| || || || || ||
A. Linearity
||
B. Stray light
|| ||
C. Absorbance accuracy
|| ||
D. Wavelength accuracy - ✔✔D
|| || || ||
In spectrophotometric analysis, what is the purpose of the reagent blank?
|| || || || || || || || || ||
A. Correct for interfering chromogens
|| || || ||
B. Correct for lipemia
|| || ||
C. Correct for protein
|| || ||
D. Correct for color contribution of the reagents - ✔✔D
|| || || || || || || || ||
In regard to bichromatic analysis, which of the following is false?
|| || || || || || || || || ||
A. Absorbance is measured at the
|| || || || ||
spectral absorbance peak for a blank
|| || || || ||
and the sample using the same
|| || || || ||
wavelength.
B. Eliminates background interferences
|| || ||
C. Sample concentration determined
|| || ||
from difference in two measured
|| || || ||
absorbances
D. Functions as a reference blank for
|| || || || || ||
each sample - ✔✔A
|| || ||
The bandpass of a spectrophotometer is
|| || || || ||
, 2
10 nm. If an instalment is set at 540 nm, the wavelengths that are permitted to impinge on
|| || || || || || || || || || || || || || || || || ||
the sample will be within what
|| || || || ||
wavelength range? ||
A. 530-540 nm
|| ||
B. 530-550 nm
|| ||
C. 535-545 nm
|| ||
D. 540-550 nm - ✔✔C
|| || || || ||
540 +/- 5nm (10nm) || || ||
Which of the following formulas is an || || || || || ||
expression of the Beer-Lambert law that is || || || || || ||
routinely applied to spectrophotometric || || ||
analysis?
A. A u X C s/As = C u
|| || || || || || || ||
B. C u X C s/As= A u
|| || || || || || ||
C. A s X C s/C u = A u
|| || || || || || || || ||
D. A = 2 - log %T - ✔✔A
|| || || || || || || ||
1 1 . In spectrophotometry, which of the following is a mathematical expression of the
|| || || || || || || || || || || || || || ||
relationship between absorbance and transmittance? || || || ||
A. A — abc || || ||
R A u/ C u = A s/C s
|| || || || || || || ||
C. A = 2 - log %T
|| || || || || ||
D. A = log %T - ✔✔C
|| || || || || ||
Which of the following is not a problem inherent in turbidimetry?
|| || || || || || || || || ||
