Competitive vs Non-Competitive Enzyme Inhibition - High Yield Biochemistry Summary

Competitive
vs
Non-Competitive
Inhibition

Competitive VS Non-competitive

Inhibitor binds on the Inhibitor binds on the
active site allosteric site
Km increases , Vmax Km remains contant ,
remains constant Vmax decreases
Effect decreases with Substrate
increased substrate concentration has no
concentration effect on it
Is reversible Usually irreversible
Example: Succinate Example: heavy metal
dehydrogenase ions (Ag+, Pb2+) binds
substrate: succinic acid to cysteinyl sulfhydryl
inhibitor: malonic acid groups of enzymes
Lineweaver–Burk plot: Lineweaver–Burk plot:
Lines intersect at Y- Lines intersect at X-
axis axis