According to the ground rules (CAP guidelines) of cell maturatio, explain the trends (important events
per stage) of erythrocyte in maturation in terms of the following:
Nuclear Changes Cytoplasmic Changes
~
Round, fine nucleus to dark, segmented nucleus. ~
Increasing cytoplasm
Promyelocyte: round ~
Cytoplasm from intensely blue (full of RNA) to grayish
(mixture of RNA and Hemoglobin) to reddish (full of
Myelocyte: oval to round and is slightly dented ~ hemoglobin, no RNA)
Metamyelocyte: indented or kidney-bean shaped Promyelocyte: Increased basophilic cytoplasm, dark blue
Band Neutrophil: elongated horseshoe Basophilic normoblast: deeper blue, richer
Granulocyte: lobulated Polychromatic normoblast: murky gray blue
(polychromatophilic)
Pink Color: accumulation of
Cell Size Hemoglobin
Blue: decreasing RNA amount
~
Larger to smaller
Orthocrhomic Normoblast: Increased salmon pink
Pronormoblast: 12-20 micrometer diameter Hemoglobin production
Basophilic Normoblast: 10-15 micrometer diameter Erythrocyte: Salmon-pink
Central pallor area (1/3 of diameter)
Polychromatic Normoblast: 10-12 micrometer diameter
Orthochromic Normoblast: 8-10 micrometer diameter
Polychromatic Erythrocyte: 8-10 micrometer diameter
Erythrocytes: 6-8 micrometer diameter
~
per stage) of erythrocyte in maturation in terms of the following:
Nuclear Changes Cytoplasmic Changes
~
Round, fine nucleus to dark, segmented nucleus. ~
Increasing cytoplasm
Promyelocyte: round ~
Cytoplasm from intensely blue (full of RNA) to grayish
(mixture of RNA and Hemoglobin) to reddish (full of
Myelocyte: oval to round and is slightly dented ~ hemoglobin, no RNA)
Metamyelocyte: indented or kidney-bean shaped Promyelocyte: Increased basophilic cytoplasm, dark blue
Band Neutrophil: elongated horseshoe Basophilic normoblast: deeper blue, richer
Granulocyte: lobulated Polychromatic normoblast: murky gray blue
(polychromatophilic)
Pink Color: accumulation of
Cell Size Hemoglobin
Blue: decreasing RNA amount
~
Larger to smaller
Orthocrhomic Normoblast: Increased salmon pink
Pronormoblast: 12-20 micrometer diameter Hemoglobin production
Basophilic Normoblast: 10-15 micrometer diameter Erythrocyte: Salmon-pink
Central pallor area (1/3 of diameter)
Polychromatic Normoblast: 10-12 micrometer diameter
Orthochromic Normoblast: 8-10 micrometer diameter
Polychromatic Erythrocyte: 8-10 micrometer diameter
Erythrocytes: 6-8 micrometer diameter
~
