MICROBIOLOGY
MODULE 3 EXAM
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Answers
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Portage Learning, Geneva College
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Module 3 Exam
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Multiple Choice Questions and Answers
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Expert-Verified Explanations
, BIOD 171 MODULE 003 COMPREHENSIVE EXAM
1. Resolution and contrast are two critical factors that influence your ability to see an object.
Explain each.
Correct Answer:
Resolution is the microscope’s ability to distinguish two points as separate entities; it depends on the
wavelength of light and the numerical aperture of the objective lens. Poor resolution causes objects to
appear merged. Contrast is the difference in light intensity between the specimen and the background;
sufficient contrast is needed to differentiate features within or between cells. Without adequate contrast,
specimens may blend with the background, making features indistinct.
Expert Rationale:
Improving resolution allows visualization of fine structural details, critical for examining small microbes or
subcellular components. Contrast enhancement techniques, like staining or specialized microscopy (e.g.,
phase contrast), make less visible organisms or structures discernible, highlighting microbiological function
or morphology.
2. A micrometer is defined as:
(one millionth of a meter, 10⁻⁶)
Answer choices:
A. 10⁻³
B. 10⁻⁶
C. 10⁻⁹
D. 10⁻¹²
Correct Answer: B. 10⁻⁶