MRO EXAM QUESTIONS AND ANSWERS WITH COMPLETE
SOLUTIONS VERIFIED LATEST UPDATE GRADED A+ 2026/2027
Federal Chain of Custody Form (CCF): 1. No CCF
What constitutes a fatal flaw? (40.199) 2. No specimen submitted with the CCF
3. No printed collector's name and no collector's signature
4. 2 separate collections using 1 CCF
5. Specimen ID numbers on bottle and form don't match
6.Broken seal or evidence of tampering (Split specimen cannot be re-designated)
7.Leakage, insufficient amount of urine (Split cannot be re-designated)
Federal Chain of Custody Form (CCF): 1. Employee's signature is omitted from the certification statement (unless refusal to sign
What are considered minor or correctable flaws? (40.203) in "Remarks" line)
2.Certifying scientist's signature omitted -(+), adulterated, substituted or invalid test
3.Non-federal form or expired CCF used, procedure must be correct.
,DOT Collection Procedures: 1. Photo ID for employee and collector
What are the basic steps of the collection process? (40.61- 2. Explain procedure
40.73) 3. Alcohol testing first
4. Remove outer clothing
5.Wash hands
6.Kit selection
7. Direct to the Bathroom
8.Pay Attention
DOT Collection Procedures: 1. Directed by DER
When must an observed collection be ordered by the 2. Attempt to
tamper
collector? (40.67) 3. Temperature on original out of range
4.Tampered with
5.Same gender
DOT Collection Procedures: a. Lab states test invalid and MRO states no medical explanation;
When must an observed collection be ordered by the b. Original (+), adulterated or substituted and split cannot be
performed employer? c. Return to Duty
d. Neg-dilute with Cr > 2mg/dL but < 5mg/dL
DOT Collection Procedures:
When must an observed collection be ordered by the MRO?
Shy Bladder: 1) Discard specimen unless temp out of range or shows signs of tampering and then
note What are DOT’s regulations regarding shy bladder (Insufficient in "remarks"
volume)? (40.193) Less than 45ML 2) Urge to drink up to 40oz in up to 3 hours
3) If Employee refuses or leaves site discontinue and note on remark line
4) Discontinue after 3 hours and note on Remarks line
, Shy Bladder: · A medical doctor within 5 days
Who performs the medical evaluation for shy bladder?
(40.193)
Shy Bladder: · To Determine the pre-existing physiologic or mental condition, blood chemistries,
What does the medical evaluation for shy bladder consist of? kidney function. NO Dehydration or anxiety
(40.193)
Shy Bladder: a) Determine if a drug user
What are DOT’s regulations regarding long-term disabilities b) Provide an alternative test
that cause the donor to be unable to provide sufficient
urine? [Note the specific regulations regarding insufficient
volume and long-term disabilities area applied for pre-
employment, return-to-work, and follow-up tests only, not for
random testing.] (40.195)
Laboratory Procedures: Immunoassay are used to screen urine drug tests.
What is the purpose of the immunoassay tests?
Laboratory Procedures: 50ng/mL
What are the cutoff levels used in federally regulated testing
Immunoassay?
THCA
Laboratory Procedures: Benzoylecgonine 150nG/mL
What are the cutoff levels used in federally regulated testing
SOLUTIONS VERIFIED LATEST UPDATE GRADED A+ 2026/2027
Federal Chain of Custody Form (CCF): 1. No CCF
What constitutes a fatal flaw? (40.199) 2. No specimen submitted with the CCF
3. No printed collector's name and no collector's signature
4. 2 separate collections using 1 CCF
5. Specimen ID numbers on bottle and form don't match
6.Broken seal or evidence of tampering (Split specimen cannot be re-designated)
7.Leakage, insufficient amount of urine (Split cannot be re-designated)
Federal Chain of Custody Form (CCF): 1. Employee's signature is omitted from the certification statement (unless refusal to sign
What are considered minor or correctable flaws? (40.203) in "Remarks" line)
2.Certifying scientist's signature omitted -(+), adulterated, substituted or invalid test
3.Non-federal form or expired CCF used, procedure must be correct.
,DOT Collection Procedures: 1. Photo ID for employee and collector
What are the basic steps of the collection process? (40.61- 2. Explain procedure
40.73) 3. Alcohol testing first
4. Remove outer clothing
5.Wash hands
6.Kit selection
7. Direct to the Bathroom
8.Pay Attention
DOT Collection Procedures: 1. Directed by DER
When must an observed collection be ordered by the 2. Attempt to
tamper
collector? (40.67) 3. Temperature on original out of range
4.Tampered with
5.Same gender
DOT Collection Procedures: a. Lab states test invalid and MRO states no medical explanation;
When must an observed collection be ordered by the b. Original (+), adulterated or substituted and split cannot be
performed employer? c. Return to Duty
d. Neg-dilute with Cr > 2mg/dL but < 5mg/dL
DOT Collection Procedures:
When must an observed collection be ordered by the MRO?
Shy Bladder: 1) Discard specimen unless temp out of range or shows signs of tampering and then
note What are DOT’s regulations regarding shy bladder (Insufficient in "remarks"
volume)? (40.193) Less than 45ML 2) Urge to drink up to 40oz in up to 3 hours
3) If Employee refuses or leaves site discontinue and note on remark line
4) Discontinue after 3 hours and note on Remarks line
, Shy Bladder: · A medical doctor within 5 days
Who performs the medical evaluation for shy bladder?
(40.193)
Shy Bladder: · To Determine the pre-existing physiologic or mental condition, blood chemistries,
What does the medical evaluation for shy bladder consist of? kidney function. NO Dehydration or anxiety
(40.193)
Shy Bladder: a) Determine if a drug user
What are DOT’s regulations regarding long-term disabilities b) Provide an alternative test
that cause the donor to be unable to provide sufficient
urine? [Note the specific regulations regarding insufficient
volume and long-term disabilities area applied for pre-
employment, return-to-work, and follow-up tests only, not for
random testing.] (40.195)
Laboratory Procedures: Immunoassay are used to screen urine drug tests.
What is the purpose of the immunoassay tests?
Laboratory Procedures: 50ng/mL
What are the cutoff levels used in federally regulated testing
Immunoassay?
THCA
Laboratory Procedures: Benzoylecgonine 150nG/mL
What are the cutoff levels used in federally regulated testing
