Chromatography
Chromatography is a physical method for separation of compounds.
Tswet, Russian botanist (referred as the father of chromatography) is
credited for the development of chromatography.
The word "chromatography" originates from Greek word Chroma
meaning 'color and graphein meaning writing.
In chromatography, the sample to be examined (called the solute or
analyte) is allowed to interact with two immiscible phases - a mobile
phase and a stationary phase.
● Stationary phase (i.e. sorbent), which does not move may be a solid
or a liquid supported on a solid.
● Mobile phase moves the sample through the stationary phase. It may
be a liquid (liquid chromatography) or a gas (gas chromatography).
All chromatographic methods involve passing a mobile phase through a
stationary (Immobile) phase. The two phases are chosen so that the
components of the sample distribute themselves between the mobile and
stationary phases to varying degrees.
Classification of chromatographic methods
, Chromatographic methods can be classified in three fundamental ways:
● Based on the shape of the chromatographic bed.
● Based on the physical state of the mobile and stationary phases,
● Based on the mechanism of separation.
Based on the shape of the chromatographic bed
There are two types of chromatography- planar chromatography and
column chromatography.
Chromatography is a physical method for separation of compounds.
Tswet, Russian botanist (referred as the father of chromatography) is
credited for the development of chromatography.
The word "chromatography" originates from Greek word Chroma
meaning 'color and graphein meaning writing.
In chromatography, the sample to be examined (called the solute or
analyte) is allowed to interact with two immiscible phases - a mobile
phase and a stationary phase.
● Stationary phase (i.e. sorbent), which does not move may be a solid
or a liquid supported on a solid.
● Mobile phase moves the sample through the stationary phase. It may
be a liquid (liquid chromatography) or a gas (gas chromatography).
All chromatographic methods involve passing a mobile phase through a
stationary (Immobile) phase. The two phases are chosen so that the
components of the sample distribute themselves between the mobile and
stationary phases to varying degrees.
Classification of chromatographic methods
, Chromatographic methods can be classified in three fundamental ways:
● Based on the shape of the chromatographic bed.
● Based on the physical state of the mobile and stationary phases,
● Based on the mechanism of separation.
Based on the shape of the chromatographic bed
There are two types of chromatography- planar chromatography and
column chromatography.
