Biol 105 Final Lab Exam IVY
Tech With 100- Correct
Answers 2025-2026
What is the secondary stain in gram staining? - ANSWER -Safranin
which is trapped in the thin layer or peptidogylcan
what shape are coccus or cocci - ANSWER -round or spherical
What is a chain of cocci ? - ANSWER -streptococci
what formation of cocci resembles that of grapes ? - ANSWER
Staphylococci
Bacillus - ANSWER -Rod shaped bacteria
Comma shaped bacteria are ? - ANSWER -Vibrio
What are spiral shaped bacteria called? - ANSWER -spirochetes
What is transformation? - ANSWER -uptake of DNA from
environment
What charge does the phospholipid bilayer have ? - ANSWER
Negative Charge
What is a competent cell? - ANSWER -a bacterial cell that can take up
DNA
Why is the cell put on ice in the transformation lab ? - ANSWER -The
ice slows down the fluid motion of the phospholipid bilayer.
Why is CaCl2 used in the transformation lab ? - ANSWER -The CaCl2
neutralizes the negative charges on the DNA and the plasma membrane
, allowing it to pass through without repulsion.
What purpose dose heat shocking serve in the transformation lab? -
ANSWER -Heat shocking opens tiny pores that allow the plasmid to
pass through.
Why is it important to not heat shock the cells too long ? - ANSWER
The heat shocking will open too many pores causing it to lyse or burst.
What feature of plasmid DNA makes it less susceptible to denaturing ? -
ANSWER -Supercoiling where strands of DNA are tightly intertwined.
What is the central dogma? - ANSWER -DNA is transcribed into RNA,
which is translated into protein
G1 phase - ANSWER -metabolic activities, building up and breaking
down macro molecules, this also the phase that initiates mitosis
S phase - ANSWER -The synthesis phase of the cell cycle; the portion
of interphase during which DNA is replicated.
Centromere - ANSWER -Region of a chromosome where the two sister
chromatids attach
G2 phase - ANSWER -Growth and preparation for cell division
M-phase - ANSWER -The phase of the cell cycle that includes mitosis
and cytokinesis.
crossing over - ANSWER -exchange of genetic material between
homologous chromosomes during prophase I of meiosis
synapsis - ANSWER -the fusion of chromosome pairs at the start of
meiosis.
Heterozygous - ANSWER -An organism that has two different alleles
for a trait
Homozygous - ANSWER -An organism that has two identical alleles
for a trait
Dominant - ANSWER -An allele that is always expressed
Recessive - ANSWER -An allele that is masked when a dominant allele
Tech With 100- Correct
Answers 2025-2026
What is the secondary stain in gram staining? - ANSWER -Safranin
which is trapped in the thin layer or peptidogylcan
what shape are coccus or cocci - ANSWER -round or spherical
What is a chain of cocci ? - ANSWER -streptococci
what formation of cocci resembles that of grapes ? - ANSWER
Staphylococci
Bacillus - ANSWER -Rod shaped bacteria
Comma shaped bacteria are ? - ANSWER -Vibrio
What are spiral shaped bacteria called? - ANSWER -spirochetes
What is transformation? - ANSWER -uptake of DNA from
environment
What charge does the phospholipid bilayer have ? - ANSWER
Negative Charge
What is a competent cell? - ANSWER -a bacterial cell that can take up
DNA
Why is the cell put on ice in the transformation lab ? - ANSWER -The
ice slows down the fluid motion of the phospholipid bilayer.
Why is CaCl2 used in the transformation lab ? - ANSWER -The CaCl2
neutralizes the negative charges on the DNA and the plasma membrane
, allowing it to pass through without repulsion.
What purpose dose heat shocking serve in the transformation lab? -
ANSWER -Heat shocking opens tiny pores that allow the plasmid to
pass through.
Why is it important to not heat shock the cells too long ? - ANSWER
The heat shocking will open too many pores causing it to lyse or burst.
What feature of plasmid DNA makes it less susceptible to denaturing ? -
ANSWER -Supercoiling where strands of DNA are tightly intertwined.
What is the central dogma? - ANSWER -DNA is transcribed into RNA,
which is translated into protein
G1 phase - ANSWER -metabolic activities, building up and breaking
down macro molecules, this also the phase that initiates mitosis
S phase - ANSWER -The synthesis phase of the cell cycle; the portion
of interphase during which DNA is replicated.
Centromere - ANSWER -Region of a chromosome where the two sister
chromatids attach
G2 phase - ANSWER -Growth and preparation for cell division
M-phase - ANSWER -The phase of the cell cycle that includes mitosis
and cytokinesis.
crossing over - ANSWER -exchange of genetic material between
homologous chromosomes during prophase I of meiosis
synapsis - ANSWER -the fusion of chromosome pairs at the start of
meiosis.
Heterozygous - ANSWER -An organism that has two different alleles
for a trait
Homozygous - ANSWER -An organism that has two identical alleles
for a trait
Dominant - ANSWER -An allele that is always expressed
Recessive - ANSWER -An allele that is masked when a dominant allele
