CHEM 121
LAB REPORT
Experiment #: 10 Urinalysis
Foundations of General Chemistry w/ Lab
Portage Learning, Geneva College
, Chemistry Lab Report
Experiment #: 10
Title: Urinalysis
Purpose: In this experiment we will conduct a chemical examination of a simulated urine
sample. The test will include several chemicals that should be routinely present in the blood or
urine, which include chloride, phosphate ions, calcium ions, ammonium ions, proteins, sugars,
and ketones.
Procedure:
Test 1: Urinalysis - Test for Chloride (Cl-):
This test measures the amount of chloride in urine and is used to evaluate and manage acid-base,
fluid, and electrolyte imbalances.
1. Chloride ion solution is put in a test tube.
2. Add a colorless solution of silver nitrate AgNO3 Cl-3.
3. White precipitate immediately formed.
Test 2: Urinalysis - Test for Phosphate (PO4-3):
The phosphate urine test measures the amount of phosphate in a sample or urine collected in a
24-hour urine test. Phosphate is an ion that contains the mineral phosphorus needed by the body
to build and repair bones and teeth, help nerves function, and make muscles contract. Excess
phosphate is filleted by the kidneys and passes out of the body in the urine. Kidney problems can
cause high or low levels of phosphate in the urine or by an overactive parathyroid gland.
1. Put sodium phosphate in the test tube.
2. Add a few drops of ammonium phosphate molybdate.
3. Note a change in color (yellow)
4. Yellow precipitate is there if nitric acid is present.
5. Place the tube in hot water bath for a few minutes.
6. Yellow solid will form which Ammonium Phosphomolybdate (NH4)3PO4 + 12 MoO3
Test 3: Urinalysis - Test for Calcium (Ca +2):
This test measures the amount of calcium in urine and is used to diagnose the cause of kidney
stones and other kidney diseases, detect malfunctioning of the parathyroid glands and monitor
disorders of the kidney.
1. Put calcium ion in test tube.
2. Requires ammonium oxalate - reagent.
3. Pour into the test tube with calcium ion (NH4) 2 C2O4 - White precipitate of calcium
oxalate CaC2O4.
This study source was downloaded by 100000902171055 from CourseHero.com on 12-01-2025 20:38:46 GMT -06:00
https://www.coursehero.com/file/210281619/Chemistry-Lab-Report-10docx/
LAB REPORT
Experiment #: 10 Urinalysis
Foundations of General Chemistry w/ Lab
Portage Learning, Geneva College
, Chemistry Lab Report
Experiment #: 10
Title: Urinalysis
Purpose: In this experiment we will conduct a chemical examination of a simulated urine
sample. The test will include several chemicals that should be routinely present in the blood or
urine, which include chloride, phosphate ions, calcium ions, ammonium ions, proteins, sugars,
and ketones.
Procedure:
Test 1: Urinalysis - Test for Chloride (Cl-):
This test measures the amount of chloride in urine and is used to evaluate and manage acid-base,
fluid, and electrolyte imbalances.
1. Chloride ion solution is put in a test tube.
2. Add a colorless solution of silver nitrate AgNO3 Cl-3.
3. White precipitate immediately formed.
Test 2: Urinalysis - Test for Phosphate (PO4-3):
The phosphate urine test measures the amount of phosphate in a sample or urine collected in a
24-hour urine test. Phosphate is an ion that contains the mineral phosphorus needed by the body
to build and repair bones and teeth, help nerves function, and make muscles contract. Excess
phosphate is filleted by the kidneys and passes out of the body in the urine. Kidney problems can
cause high or low levels of phosphate in the urine or by an overactive parathyroid gland.
1. Put sodium phosphate in the test tube.
2. Add a few drops of ammonium phosphate molybdate.
3. Note a change in color (yellow)
4. Yellow precipitate is there if nitric acid is present.
5. Place the tube in hot water bath for a few minutes.
6. Yellow solid will form which Ammonium Phosphomolybdate (NH4)3PO4 + 12 MoO3
Test 3: Urinalysis - Test for Calcium (Ca +2):
This test measures the amount of calcium in urine and is used to diagnose the cause of kidney
stones and other kidney diseases, detect malfunctioning of the parathyroid glands and monitor
disorders of the kidney.
1. Put calcium ion in test tube.
2. Requires ammonium oxalate - reagent.
3. Pour into the test tube with calcium ion (NH4) 2 C2O4 - White precipitate of calcium
oxalate CaC2O4.
This study source was downloaded by 100000902171055 from CourseHero.com on 12-01-2025 20:38:46 GMT -06:00
https://www.coursehero.com/file/210281619/Chemistry-Lab-Report-10docx/
