PORTAGE LEARNING BIOD 171
MICROBIOLOGY LAB NOTEBOOK
COMPREHENSIVE SCRIPT 2026 COMPLETE
QUESTIONS AND SOLUTIONS GRADED A+
⩥ NON-LACTOSE FERMENTERS on the EMB and McConkey agars
appear ____________________. Answer: Colorless
⩥ On EMB agar, LACTOSE FERMENTERS appear
____________________ (small and large acid production). Answer:
Metallic Green for large acid production Purple for small acid
production
⩥ Beta-hemolysis (complete cell lysis). Answer: Clearing around growth
⩥ Alpha-hemolysis (partial cell lysis). Answer: Greening around growth
⩥ Gamma-hemolysis (no cell lysis). Answer: No change in the medium
⩥ Positive Result For Catalase Test. Answer: Bubbles
⩥ Negative Result For Catalase Test. Answer: No Bubbles
, ⩥ Positive Result For Coagulase Test. Answer: Plasma forms a clot
⩥ Negative Result For Coagulase Test. Answer: Plasma remains liquid
⩥ Positive Result For Gelatin Liquidification Test. Answer: Gelatin
Liquified
⩥ Negative Result For Gelatin Liquidification Test. Answer: Gelatin
remained solid
⩥ What is the importance of the Coagulase Test?. Answer: It
distinguishes Staphylococcus aureus from other Gram positive cocci
⩥ What is the enzyme used in the Gelatin Liquidification Test?. Answer:
Gelatinase
⩥ Positive Result For Hydrolysis of Milk Protein Test. Answer: clearing
around bacteria
⩥ What is the ENZYME used in the Hydrolysis of Milk Protein Test?.
Answer: Casease
MICROBIOLOGY LAB NOTEBOOK
COMPREHENSIVE SCRIPT 2026 COMPLETE
QUESTIONS AND SOLUTIONS GRADED A+
⩥ NON-LACTOSE FERMENTERS on the EMB and McConkey agars
appear ____________________. Answer: Colorless
⩥ On EMB agar, LACTOSE FERMENTERS appear
____________________ (small and large acid production). Answer:
Metallic Green for large acid production Purple for small acid
production
⩥ Beta-hemolysis (complete cell lysis). Answer: Clearing around growth
⩥ Alpha-hemolysis (partial cell lysis). Answer: Greening around growth
⩥ Gamma-hemolysis (no cell lysis). Answer: No change in the medium
⩥ Positive Result For Catalase Test. Answer: Bubbles
⩥ Negative Result For Catalase Test. Answer: No Bubbles
, ⩥ Positive Result For Coagulase Test. Answer: Plasma forms a clot
⩥ Negative Result For Coagulase Test. Answer: Plasma remains liquid
⩥ Positive Result For Gelatin Liquidification Test. Answer: Gelatin
Liquified
⩥ Negative Result For Gelatin Liquidification Test. Answer: Gelatin
remained solid
⩥ What is the importance of the Coagulase Test?. Answer: It
distinguishes Staphylococcus aureus from other Gram positive cocci
⩥ What is the enzyme used in the Gelatin Liquidification Test?. Answer:
Gelatinase
⩥ Positive Result For Hydrolysis of Milk Protein Test. Answer: clearing
around bacteria
⩥ What is the ENZYME used in the Hydrolysis of Milk Protein Test?.
Answer: Casease