ASU BIO 340 EXAM 3 PRACTICE SCRIPT UPDATED 2026 TESTED ANSWERS

ASU BIO 340 EXAM 3 PRACTICE SCRIPT
UPDATED 2026 TESTED ANSWERS

⫸ d. Denaturation, annealing, extension Answer: The correct order of
steps in a PCR cycle is:


a. Extension, denaturation, annealing
b. Annealing, extension, denaturation
c. Annealing, denaturation, extension
d. Denaturation, annealing, extension


⫸ c. Amplify a small amount of DNA sequence Answer: The goal of
the polymerase chain reaction (PCR) is to:


a. Sequence DNA
b. Digest bacterial plasmids
c. Amplify a small amount of DNA sequence
d. Cleave DNA molecules


⫸ b. Two strands of DNA double helix are held together by covalent
bonds

, (hydrogen bonds, not covalent bonds) Answer: Which of the following
is incorrect?


a. Cytosine pairs with Guanine
b. Two strands of DNA double helix are held together by covalent bonds
c. Nucleotides in DNA consist of deoxyribose sugar, phosphate group
and a nitrogenous base
d. Adenine pairs with Thymine


⫸ d. A phosphate group, a nitrogenous base, a deoxyribose Answer:
What are the 3 main components of a DNA nucleotide?


a. An alcohol functional group, a nitrogenous base, a ribose
b. An alcohol functional group, a nitrogenous base, a deoxyribose
c. A phosphate group, a nitrogenous base, a ribose
d. A phosphate group, a nitrogenous base, a deoxyribose


⫸ d. 95 Answer: At which temperature does a DNA double helix
denature?


a. 15
b. 85
c. 75
d. 95