“Lab 2 Culturing & Aseptic Technique BIO250L”
Student Name: Click here to enter text.
Access Code (located on the underside of the lid of your lab kit): Click here to enter text.
“Pre-Lab Questions”
1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one
experimental way you can test your practices to confirm that you are using proper aseptic
technique?
By carryimg put streak plating once can spred out the bacterial cells
enough to be able to tell if the procedure followed was aseptic or not.
2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment?
a. Through various forms of radiation such as gamma and UV
b. Through the use of Wet heat such as pasterurization
c. Through the use of dry heat for example over flames i.e Bunsen
burner
3. For each inoculation tool, give one scenario in which use of that tool would be appropriate.
We can use a wire loop of inoculation needle for inoculation; wire loops
would serve best when inoculating plates while needles wopuld serve best
as testtubee inoculation tools for example forming stab lines.
4. Why don’t microorganisms in cultures exhibit constant exponential growth? What are some
steps you could take to extend the lifespan of a microbial culture?
, “Lab 2 Culturing & Aseptic Technique BIO250L”
This is due to the inevitable total consumptrion of nutrients and resources
l;eading to the cells eventually starving and dying out. In order to extend
their lifespans, one can continuously nourish the cells in culture with the
necessary nutrients.
5. Using a textbook or a reputable online source, describe how lab cultures are maintained in a
continual pattern of growth. Focus particularly on those used in biotechnology, such as E. coli,
which is used to make human insulin.
The maintenance of laboratory cultures of bacteria used in biotechnology
involves routinely supplying them with the best circumstances for
development and multiplication. This entails maintaining the right pH
levels, temperatures, and nutrients. For instance, when E. coli bacteria are
used to produce insulin, the culture is often cultivated in a liquid medium
that contains vital nutrients such amino acids, carbohydrates, vitamins,
and minerals. The temperature is maintained at 37 °C, which is ideal for E.
coli growth. Additionally, the medium's pH is kept at a neutral level of
roughly 7.0. The bacteria are routinely subcultured, which entails moving a
tiny section of the bacterial culture to a new media, to maintain a steady
pattern of development. This keeps the availability of nutrients for the
bacteria's continued growth adequate and helps prevent the buildup of
hazardous metabolic waste products. Last but not least, it's critical to often
examine the cleanliness of the bacterial culture to make sure it stays free of
impurities that can affect its growth or the caliber of the final output (in this
Student Name: Click here to enter text.
Access Code (located on the underside of the lid of your lab kit): Click here to enter text.
“Pre-Lab Questions”
1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one
experimental way you can test your practices to confirm that you are using proper aseptic
technique?
By carryimg put streak plating once can spred out the bacterial cells
enough to be able to tell if the procedure followed was aseptic or not.
2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment?
a. Through various forms of radiation such as gamma and UV
b. Through the use of Wet heat such as pasterurization
c. Through the use of dry heat for example over flames i.e Bunsen
burner
3. For each inoculation tool, give one scenario in which use of that tool would be appropriate.
We can use a wire loop of inoculation needle for inoculation; wire loops
would serve best when inoculating plates while needles wopuld serve best
as testtubee inoculation tools for example forming stab lines.
4. Why don’t microorganisms in cultures exhibit constant exponential growth? What are some
steps you could take to extend the lifespan of a microbial culture?
, “Lab 2 Culturing & Aseptic Technique BIO250L”
This is due to the inevitable total consumptrion of nutrients and resources
l;eading to the cells eventually starving and dying out. In order to extend
their lifespans, one can continuously nourish the cells in culture with the
necessary nutrients.
5. Using a textbook or a reputable online source, describe how lab cultures are maintained in a
continual pattern of growth. Focus particularly on those used in biotechnology, such as E. coli,
which is used to make human insulin.
The maintenance of laboratory cultures of bacteria used in biotechnology
involves routinely supplying them with the best circumstances for
development and multiplication. This entails maintaining the right pH
levels, temperatures, and nutrients. For instance, when E. coli bacteria are
used to produce insulin, the culture is often cultivated in a liquid medium
that contains vital nutrients such amino acids, carbohydrates, vitamins,
and minerals. The temperature is maintained at 37 °C, which is ideal for E.
coli growth. Additionally, the medium's pH is kept at a neutral level of
roughly 7.0. The bacteria are routinely subcultured, which entails moving a
tiny section of the bacterial culture to a new media, to maintain a steady
pattern of development. This keeps the availability of nutrients for the
bacteria's continued growth adequate and helps prevent the buildup of
hazardous metabolic waste products. Last but not least, it's critical to often
examine the cleanliness of the bacterial culture to make sure it stays free of
impurities that can affect its growth or the caliber of the final output (in this
