BIOD 171 MICROBIOLOGY LAB NOTEBOOK
BIOD171 Microbiology Lab
Notebook.
Portage Learning
BIO 171- Microbiology
Lab Notebook
Lab Notebook Bookmarks (click to navigate):
Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
,BIOD 171 MICROBIOLOGY LAB NOTEBOOK
Lab 1 Notebook
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Title: Introduction to Medical Microbiology
Objective: The objective of this lab will be to familiarize myself with how to conduct
experiments and practice proper safety procedures while in a Lab. Cultivation, Identification,
and Evaluation are the 3 main principles discussed when conducting any experiment.
Procedure: Our instructor introduces us to the foundation of microbiology lab with an
introduction to basic equipment and safety rules. We are also show how to keep our Lab
Notebook formatted.
Notes: Basic Equipment
❖ Cleaning – need to work in a sterile environment to keep everyone healthy
o Main tool: Autoclave 125 degrees C (looks like a giant dryer machine with a thick
door)
▪ Come in variety of sizes
▪ Uses heat, pressure, and steam to sterilize environment
▪ Put tools in before you use for a lab OR put contaminated tools to kill
bacteria
▪ Not just dry air, steam is more efficient
❖ Growing- how to grow the pathogens and bacteria?
❖ Two Main Ways:
,BIOD 171 MICROBIOLOGY LAB NOTEBOOK
➢ Fixed incubator 37 degrees C (Chamber- looks like a mini fridge)
▪ Stored Petri dishes, which contain the samples
▪ Airtight seal, and metal door to keep dark conditions
➢ Shaker incubator 37 degrees C
▪ Used to take a growth media, which is liquid nutrient broth
▪ After inoculating bacteria, put it in here, and shaker shakes and rotates culture in a
swirling fashion
▪ Helps arete it and help bacteria grow under optimal conditions
❖ Visualizing- How do we see these bacteria and things?
➢ Microscopy
▪ Beneficial to seeing bacteria
❖ Storing
➢ Refrigerator 4 degree C
▪ Temperature slows bacterial growth, which helps preserve samples and keep them
for long term.
• Test tubes which may have liquid broth, petri dishes which are wrapped airtight,
or test tube racks
General Lab Safety
❖ Never eat or drink in lab
➢ Contamination risks
❖ Use PPE
➢ Latex (or nitrile (no latex) or thermal gloves (handle anything hot) or cold gloves (liquid
nitrogen)
➢ Eyewear protection
➢ Lab coat (worn when working with chemicals that can be spilled)
, BIOD 171 MICROBIOLOGY LAB NOTEBOOK
❖ Never leave lab while wearing PPE
➢ Spreads and containments other areas
▪ Public hallway, restrooms, cafeteria, etc.
Results: In this Lab, the instructor was able to educate us on proper Lab room procedures and
safety. We were given an introduction in learning the basic equipment we will use to cultivate,
identify, and examine bacteria.
BIOD171 Microbiology Lab
Notebook.
Portage Learning
BIO 171- Microbiology
Lab Notebook
Lab Notebook Bookmarks (click to navigate):
Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
,BIOD 171 MICROBIOLOGY LAB NOTEBOOK
Lab 1 Notebook
Back to Home Page
Title: Introduction to Medical Microbiology
Objective: The objective of this lab will be to familiarize myself with how to conduct
experiments and practice proper safety procedures while in a Lab. Cultivation, Identification,
and Evaluation are the 3 main principles discussed when conducting any experiment.
Procedure: Our instructor introduces us to the foundation of microbiology lab with an
introduction to basic equipment and safety rules. We are also show how to keep our Lab
Notebook formatted.
Notes: Basic Equipment
❖ Cleaning – need to work in a sterile environment to keep everyone healthy
o Main tool: Autoclave 125 degrees C (looks like a giant dryer machine with a thick
door)
▪ Come in variety of sizes
▪ Uses heat, pressure, and steam to sterilize environment
▪ Put tools in before you use for a lab OR put contaminated tools to kill
bacteria
▪ Not just dry air, steam is more efficient
❖ Growing- how to grow the pathogens and bacteria?
❖ Two Main Ways:
,BIOD 171 MICROBIOLOGY LAB NOTEBOOK
➢ Fixed incubator 37 degrees C (Chamber- looks like a mini fridge)
▪ Stored Petri dishes, which contain the samples
▪ Airtight seal, and metal door to keep dark conditions
➢ Shaker incubator 37 degrees C
▪ Used to take a growth media, which is liquid nutrient broth
▪ After inoculating bacteria, put it in here, and shaker shakes and rotates culture in a
swirling fashion
▪ Helps arete it and help bacteria grow under optimal conditions
❖ Visualizing- How do we see these bacteria and things?
➢ Microscopy
▪ Beneficial to seeing bacteria
❖ Storing
➢ Refrigerator 4 degree C
▪ Temperature slows bacterial growth, which helps preserve samples and keep them
for long term.
• Test tubes which may have liquid broth, petri dishes which are wrapped airtight,
or test tube racks
General Lab Safety
❖ Never eat or drink in lab
➢ Contamination risks
❖ Use PPE
➢ Latex (or nitrile (no latex) or thermal gloves (handle anything hot) or cold gloves (liquid
nitrogen)
➢ Eyewear protection
➢ Lab coat (worn when working with chemicals that can be spilled)
, BIOD 171 MICROBIOLOGY LAB NOTEBOOK
❖ Never leave lab while wearing PPE
➢ Spreads and containments other areas
▪ Public hallway, restrooms, cafeteria, etc.
Results: In this Lab, the instructor was able to educate us on proper Lab room procedures and
safety. We were given an introduction in learning the basic equipment we will use to cultivate,
identify, and examine bacteria.
