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1. Describe the structure and function of the nucleoid region in prokaryotic cells.
Correct Answer:
The nucleoid is an irregularly shaped region in a prokaryotic cell that contains
most or all of the bacterial genetic material (DNA). It is not membrane-bound.
2. Explain the role of plasmids in bacterial survival and adaptation, including
their clinical significance.
Correct Answer:
Plasmids are small, circular, double-stranded DNA molecules separate from the
main chromosome that often carry genes for antibiotic resistance, virulence, and
survival advantages.
3. Compare ribosomes in prokaryotic and eukaryotic cells with respect to size
and cellular location.
Correct Answer:
Prokaryotic ribosomes are 70S and are located in the cytoplasm.
Eukaryotic ribosomes are larger (80S) and are found free in the cytoplasm or
attached to the endoplasmic reticulum.
,4. What are bacterial inclusions, and how do they contribute to survival in
fluctuating environments?
Correct Answer:
Inclusions are storage structures in prokaryotic cells that store excess nutrients,
helping bacteria survive unstable conditions and reducing osmotic pressure.
5. Define bacterial endospores and explain their biological purpose.
Correct Answer:
Endospores are dormant, highly resistant structures formed by certain bacteria to
protect genetic material during unfavorable conditions such as heat, toxins, or
nutrient depletion.
6. Describe the structure and primary functions of the bacterial plasma
membrane.
Correct Answer:
The plasma membrane encloses the cytoplasm and internal structures and
regulates the movement of substances into and out of the cell. It is composed of
fluid lipids and proteins.
7. Explain why prokaryotic cells lack a nuclear envelope.
Correct Answer:
Prokaryotes lack a nuclear envelope because their genetic material is located in
the nucleoid region rather than within a membrane-bound nucleus.
8. Discuss the function of the bacterial cell wall.
, Correct Answer:
The cell wall protects bacteria from environmental stress, maintains cell shape,
and prevents osmotic lysis.
9. Compare the cell wall structure of Gram-positive and Gram-negative bacteria.
Correct Answer:
Gram-positive bacteria have thick peptidoglycan layers containing teichoic acids.
Gram-negative bacteria have thinner peptidoglycan layers and an outer
membrane containing lipopolysaccharides.
10. Identify the role of teichoic acids in Gram-positive bacteria.
Correct Answer:
Teichoic acids strengthen the cell wall and contribute to cell rigidity and antigenic
properties.
11. Explain the significance of lipopolysaccharides (LPS) in Gram-negative
bacteria.
Correct Answer:
LPS form part of the outer membrane and contribute to structural integrity and
pathogenicity, including endotoxin activity.
12. Describe the structure and function of bacterial flagella.
Correct Answer:
Flagella are long, whip-like structures that enable bacterial movement in liquid
environments.
, 13. Differentiate between fimbriae and pili.
Correct Answer:
Fimbriae are short, numerous structures used for attachment and biofilm
formation.
Pili are longer, fewer structures involved in attachment and DNA transfer between
bacteria.
14. Explain the role of fimbriae in bacterial pathogenicity.
Correct Answer:
Fimbriae help bacteria adhere to host tissues, promoting colonization, infectivity,
and virulence.
15. Describe the role of pili in genetic exchange.
Correct Answer:
Pili facilitate horizontal gene transfer through conjugation between bacterial cells.
16. Define chemotaxis.
Correct Answer:
Chemotaxis is bacterial movement in response to chemical signals in the
environment.
17. Differentiate between positive and negative chemotaxis.
Correct Answer:
Positive chemotaxis is movement toward a chemical attractant.
Negative chemotaxis is movement away from a chemical repellent.