Page |1
Bacterial identification Virtual Lab
Questions and Correct Answers/ Latest
Update / Already Graded
What's the overall purpose of this virtual lab?
Ans: Familiarize with science and techniques used to identify
different types of bacteria based on their DNA sequence
What are the four basic steps involved in this bacterial identification
lab?
Ans: 1. Prepare sample from patient and isolate whole
bacterial DNA
2. Make many copies of desired piece of DNA
3. Sequence the DNA
4. Analyze the sequence and identify the bacteria
What is 16s rDNA and how is it used to identify species of bacteria?
Ans: 16s rDNA is a piece of DNA used for identifying bacteria
the region codes for a small subunit of ribosomal RNA.
Identification relies on matching the sequence form your
sample against a database of all known 16s rDNA sequences
All rights reserved © 2025/ 2026 |
, Page |2
As the pathology lab technician what's your task in this virtual lab?
Ans: Identify a bacterial sample received from a clinician
Extracting DNA involves which initial step?
Ans: Dissolving the cell wall with a digestive buffer (in white
capped bottle)
What is the wire ring used for?
Ans: Obtain a sample form the Petri dish (extracts a colony)
Why are the proteolytic enzymes necessary?
Ans: Buffer is a digestive enzyme that east the cell wall in
order to extract the bacterial DNA
Why do you need to inactivate the proteolytic enzymes and how do
you do it?
Ans: Other enzymes are used in next step and enzyme is
denatured by heating sample in a H2O bath @ 100 °C
After removing the enzymes why do you spin the sample in a
centrifuge?
All rights reserved © 2025/ 2026 |
Bacterial identification Virtual Lab
Questions and Correct Answers/ Latest
Update / Already Graded
What's the overall purpose of this virtual lab?
Ans: Familiarize with science and techniques used to identify
different types of bacteria based on their DNA sequence
What are the four basic steps involved in this bacterial identification
lab?
Ans: 1. Prepare sample from patient and isolate whole
bacterial DNA
2. Make many copies of desired piece of DNA
3. Sequence the DNA
4. Analyze the sequence and identify the bacteria
What is 16s rDNA and how is it used to identify species of bacteria?
Ans: 16s rDNA is a piece of DNA used for identifying bacteria
the region codes for a small subunit of ribosomal RNA.
Identification relies on matching the sequence form your
sample against a database of all known 16s rDNA sequences
All rights reserved © 2025/ 2026 |
, Page |2
As the pathology lab technician what's your task in this virtual lab?
Ans: Identify a bacterial sample received from a clinician
Extracting DNA involves which initial step?
Ans: Dissolving the cell wall with a digestive buffer (in white
capped bottle)
What is the wire ring used for?
Ans: Obtain a sample form the Petri dish (extracts a colony)
Why are the proteolytic enzymes necessary?
Ans: Buffer is a digestive enzyme that east the cell wall in
order to extract the bacterial DNA
Why do you need to inactivate the proteolytic enzymes and how do
you do it?
Ans: Other enzymes are used in next step and enzyme is
denatured by heating sample in a H2O bath @ 100 °C
After removing the enzymes why do you spin the sample in a
centrifuge?
All rights reserved © 2025/ 2026 |
