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Bacterial Identification Lab Questions and
Correct Answers/ Latest Update / Already
Graded
What is the overall purpose of this virtual lab?
Ans: To familiarize students with the science and technology
used to identify types of DNA based on their DNA sequence.
What are the four basic steps involved in this bacterial identification
lab?
Ans: Prepare a sample and isolate the whole DNA, make copies
of it, sequence the DNA, analyze and identify
What is "16S rDNA," and how is it used to identify species of bacteria?
Ans: The piece of DNA used for identifying bacteria; The
identification relies on matching the sequence from your
sample against a database of all known 16S rDNA sequences
As the pathology lab technician, what is your task in this virtual lab?
Ans: to identify a bacterial sample received from a clinician.
Extracting DNA involves which initial step?
All rights reserved © 2025/ 2026 |
, Page |2
Ans: dissolving the cell wall with a digestive buffer (in the
white-capped bottle) available as a commercial kit
What is the wire ring used for?
Ans:
Why are the proteolytic enzymes necessary?
Ans: The buffer contains proteolytic enzymes that eat the cell
wall.
Why do you then need to inactivate the proteolytic enzymes and how
do you do it?
Ans: Since we will be using other enzymes in the next step, we
need to get rid of the proteolytic enzymes.
After removing the enzymes, why do you spin the sample in the
centrifuge?
Ans: DNA is lighter than the cellular debris floating around
from the step that destroyed the cellular wall. The centrifuge
allows us to safely remove the DNA from the microcentrifuge
tube.
All rights reserved © 2025/ 2026 |
Bacterial Identification Lab Questions and
Correct Answers/ Latest Update / Already
Graded
What is the overall purpose of this virtual lab?
Ans: To familiarize students with the science and technology
used to identify types of DNA based on their DNA sequence.
What are the four basic steps involved in this bacterial identification
lab?
Ans: Prepare a sample and isolate the whole DNA, make copies
of it, sequence the DNA, analyze and identify
What is "16S rDNA," and how is it used to identify species of bacteria?
Ans: The piece of DNA used for identifying bacteria; The
identification relies on matching the sequence from your
sample against a database of all known 16S rDNA sequences
As the pathology lab technician, what is your task in this virtual lab?
Ans: to identify a bacterial sample received from a clinician.
Extracting DNA involves which initial step?
All rights reserved © 2025/ 2026 |
, Page |2
Ans: dissolving the cell wall with a digestive buffer (in the
white-capped bottle) available as a commercial kit
What is the wire ring used for?
Ans:
Why are the proteolytic enzymes necessary?
Ans: The buffer contains proteolytic enzymes that eat the cell
wall.
Why do you then need to inactivate the proteolytic enzymes and how
do you do it?
Ans: Since we will be using other enzymes in the next step, we
need to get rid of the proteolytic enzymes.
After removing the enzymes, why do you spin the sample in the
centrifuge?
Ans: DNA is lighter than the cellular debris floating around
from the step that destroyed the cellular wall. The centrifuge
allows us to safely remove the DNA from the microcentrifuge
tube.
All rights reserved © 2025/ 2026 |
