ASCP MLS Blood Bank Questions and
Answers | Latest Version | 2025/2026 |
Correct & Verified
Which antigen is most commonly associated with severe hemolytic disease of the fetus and
newborn (HDFN)?
A. Kell (K)
B. D antigen
C. Lewis (Lea)
D. M antigen
✔✔B. D antigen
Which immunoglobulin class is primarily responsible for causing acute hemolytic transfusion
reactions?
A. IgA
B. IgM
C. IgE
D. IgG
✔✔B. IgM
Which phase of compatibility testing is most effective for detecting IgG antibodies?
A. Immediate spin
B. Room temperature
1
,C. Antiglobulin (AHG) phase
D. Saline phase
✔✔C. Antiglobulin (AHG) phase
Which blood group system is most polymorphic after ABO and Rh?
A. Kell
B. Kidd
C. Duffy
D. MNS
✔✔D. MNS
Which antibody is commonly implicated in delayed hemolytic transfusion reactions?
A. Anti-K
B. Anti-Lea
C. Anti-Jka
D. Anti-P1
✔✔C. Anti-Jka
Which reagent is required to detect incomplete (IgG) antibodies in vitro?
A. Saline
B. Albumin
C. Antihuman globulin (AHG)
D. Enzyme reagent
2
,✔✔C. Antihuman globulin (AHG)
Which blood group antigen is destroyed by enzyme treatment?
A. Kell
B. Duffy
C. Kidd
D. Rh
✔✔B. Duffy
Which condition is most strongly associated with cold autoantibodies?
A. Autoimmune hemolytic anemia
B. Cold agglutinin disease
C. Hemophilia
D. Thalassemia
✔✔B. Cold agglutinin disease
Which antibody is naturally occurring and usually IgM?
A. Anti-D
B. Anti-K
C. Anti-A
D. Anti-Fya
✔✔C. Anti-A
3
, Which red cell preservative solution extends shelf life to 42 days?
A. CPD
B. ACD
C. CPDA-1
D. SAG-M
✔✔D. SAG-M
Which component is best for treating a patient with thrombocytopenia?
A. Packed RBCs
B. Platelet concentrate
C. Fresh frozen plasma
D. Cryoprecipitate
✔✔B. Platelet concentrate
Which test confirms the presence of complement on red cells?
A. Direct antiglobulin test (DAT)
B. Antibody screen
C. Crossmatch
D. Antibody identification panel
✔✔A. Direct antiglobulin test (DAT)
Which antibody is most commonly associated with mild hemolytic transfusion reactions?
A. Anti-M
4
Answers | Latest Version | 2025/2026 |
Correct & Verified
Which antigen is most commonly associated with severe hemolytic disease of the fetus and
newborn (HDFN)?
A. Kell (K)
B. D antigen
C. Lewis (Lea)
D. M antigen
✔✔B. D antigen
Which immunoglobulin class is primarily responsible for causing acute hemolytic transfusion
reactions?
A. IgA
B. IgM
C. IgE
D. IgG
✔✔B. IgM
Which phase of compatibility testing is most effective for detecting IgG antibodies?
A. Immediate spin
B. Room temperature
1
,C. Antiglobulin (AHG) phase
D. Saline phase
✔✔C. Antiglobulin (AHG) phase
Which blood group system is most polymorphic after ABO and Rh?
A. Kell
B. Kidd
C. Duffy
D. MNS
✔✔D. MNS
Which antibody is commonly implicated in delayed hemolytic transfusion reactions?
A. Anti-K
B. Anti-Lea
C. Anti-Jka
D. Anti-P1
✔✔C. Anti-Jka
Which reagent is required to detect incomplete (IgG) antibodies in vitro?
A. Saline
B. Albumin
C. Antihuman globulin (AHG)
D. Enzyme reagent
2
,✔✔C. Antihuman globulin (AHG)
Which blood group antigen is destroyed by enzyme treatment?
A. Kell
B. Duffy
C. Kidd
D. Rh
✔✔B. Duffy
Which condition is most strongly associated with cold autoantibodies?
A. Autoimmune hemolytic anemia
B. Cold agglutinin disease
C. Hemophilia
D. Thalassemia
✔✔B. Cold agglutinin disease
Which antibody is naturally occurring and usually IgM?
A. Anti-D
B. Anti-K
C. Anti-A
D. Anti-Fya
✔✔C. Anti-A
3
, Which red cell preservative solution extends shelf life to 42 days?
A. CPD
B. ACD
C. CPDA-1
D. SAG-M
✔✔D. SAG-M
Which component is best for treating a patient with thrombocytopenia?
A. Packed RBCs
B. Platelet concentrate
C. Fresh frozen plasma
D. Cryoprecipitate
✔✔B. Platelet concentrate
Which test confirms the presence of complement on red cells?
A. Direct antiglobulin test (DAT)
B. Antibody screen
C. Crossmatch
D. Antibody identification panel
✔✔A. Direct antiglobulin test (DAT)
Which antibody is most commonly associated with mild hemolytic transfusion reactions?
A. Anti-M
4
