BICH 410 EXAM 2 QUESTIONS ANSWERED CORRECTLY LATEST UPDATE 2026
What is used to sequence entire proteins?
a. chymotrypsin peptides
b. overlapping peptide sequences
c. labeled peptides
d. polypeptides
e. synthetic peptides - Answers b. overlapping peptide sequences
Two-dimensional electrophoresis is a combination of:
a. isoelectric focusing and SDS-PAGE.
b. isoelectric focusing and ion-exchange chromatography.
c. SDS-PAGE and affinity chromatography.
d. ion-exchange chromatography and affinity chromatography.
e. ion-exchange chromatography and SDS-PAGE. - Answers a. isoelectric focusing and SDS-PAGE.
What level of protein structure hierarchy contains α helices, β sheets, and turns?
a. supersecondary
b. quaternary
c. primary
d. secondary
e. tertiary - Answers d. secondary
What does an amino acid consist of?
a. carboxylic acid group, ε carbon, nitro group, hydrogen atom
b. carbonyl group, α carbon, amino group, oxygen atom, distinctive R group
c. carboxylic acid group, δ carbon, Schiff base, hydrogen atom, distinctive R group
d. carboxylic acid group, α carbon, amino group, hydrogen atom, distinctive R group
e. alcohol group, α carbon, amino group, nitrogen atom, distinctive R group, hydrogen atom -
Answers d. carboxylic acid group, α carbon, amino group, hydrogen atom, distinctive R group
An example of closed symmetry is
a. actin filaments
b. antibodies
c. sickle cell hemoglobin filaments
d. microtubules - Answers b. antibodies
Which of the following is CORRECT, regarding Hb and Mb?
a. the R-state of Mb binds oxygen
b. the R-state of Hb binds oxygen
c. the T-state of Hb transports oxygen
d. the T-state of Mb transports oxygen
e. the R-state of Hb stores oxygen - Answers b. the R-state of Hb binds oxygen
What is the difference between β strands and loops?
a. Loops do not have a three-dimensional structure.
b. Loops have a greater molecular weight.
c. Loops do not have hydrogen bonds between side chains.
d. Loops do not have regular backbone phi and psi angles.
e. Loops do not have amino acid residues. - Answers d. Loops do not have regular backbone phi and
psi angles.
The allosteric effector, 2,3-BPG, makes
a. the O2 binding curve of Mb hyperbolic
b. the O2 binding curve of Hb-F sigmoidal
c. the O2 binding curve of Hb sigmoidal
d. the O2 binding curve of Mb sigmoidal
e. the binding of O2 to Hb stronger - Answers c. the O2 binding curve of Hb sigmoidal
What is a structure of the hemoglobin molecule?
a. α2 dimer
b. α2β2 tetramer
c. α1β2 trimer
d. β2 dimer
e. α2β1 trimer - Answers b. α2β2 tetramer
, What chemical is used to release the N-terminal amino acid of a polypeptide?
a. Iodoacetic acid
b. phenyl isothiocyanate
c. Sodium dodecyl sulfate
d. Cyanogen bromide
e. Beta mercaptoethanol - Answers b. phenyl isothiocyanate
What residues are hydrolyzed on their C-side during trypsin digestion?
a. Asn and Lys
b. Lys and Val
c. Ser and Met
d. Val and Ser
e. Arg and Lys - Answers e. Arg and Lys
Choose the order of the atoms that lie in the amide plane that links amino acids.
a. Cα, Cα2, Cα3, NH, CO
b. Cα, CO, NH, Cα3
c. Cα, Cα2, NH, CO
d. Cα3, CO, NH, Cα2
e. Cα, CO, NH, Cα2 - Answers e. Cα, CO, NH, Cα2
What method can be used to separate proteins by specific binding with other molecules?
a. gel-filtration chromatography
b. affinity chromatography
c. SDS-PAGE
d. ion-exchange chromatography
e. dialysis - Answers b. affinity chromatography
A good example of open symmetry is
a. chaperones
b. antibodies
c. hemoglobin
d. microtubules - Answers d. microtubules
What is the amino-terminal residue and what is the carboxyl-terminal residue in the sequence of
amino acids Gly-Tyr-Gly-Phe-Leu?
a. There are no N-terminal and C-terminal residues in the sequences of amino acids.
b. Leucine is N-terminal and glycine is C-terminal.
c. Phenylalanine is N-terminal and leucine is C-terminal.
d. Tyrosine is N-terminal and leucine is C-terminal.
e. Glycine is N-terminal and leucine is C-terminal. - Answers e. Glycine is N-terminal and leucine is C-
terminal.
In the womb, the fetus obtains oxygen from the mother through the placenta by
a. tighter binding of Hb F to 2,3-BPG
b. joined veins and capillaries, with Hb transporting O2 from the mother
c. tighter binding of Hb F to O2
d. weaker binding of Hb F to 2,3-BPG
e. weaker binding of Hb F to 2,3-BPG and tighter binding of Hb F to O2 - Answers e. weaker binding of
Hb F to 2,3-BPG and tighter binding of Hb F to O2
Which of the following about the influenza HA structure is TRUE?
a. The conformational change involves movement of the F8 helix.
b. The structure is monomeric, until the conformational change is triggered.
c. The trimer uses a banana-shape to bind and bend the membrane.
d. The conformational change results in relocation of the fusion peptides into the target membrane.
e. The trimer is held together with a collagen-like triple helix. - Answers d. The conformational change
results in relocation of the fusion peptides into the target membrane.
_____ is added prior to gel electrophoresis to denature proteins.
a. Polyacrylamide
b. Cyanogen bromide
c. SDS
d. β-mercaptoethanol
e. Coomassie Blue - Answers c. SDS
What is used to sequence entire proteins?
a. chymotrypsin peptides
b. overlapping peptide sequences
c. labeled peptides
d. polypeptides
e. synthetic peptides - Answers b. overlapping peptide sequences
Two-dimensional electrophoresis is a combination of:
a. isoelectric focusing and SDS-PAGE.
b. isoelectric focusing and ion-exchange chromatography.
c. SDS-PAGE and affinity chromatography.
d. ion-exchange chromatography and affinity chromatography.
e. ion-exchange chromatography and SDS-PAGE. - Answers a. isoelectric focusing and SDS-PAGE.
What level of protein structure hierarchy contains α helices, β sheets, and turns?
a. supersecondary
b. quaternary
c. primary
d. secondary
e. tertiary - Answers d. secondary
What does an amino acid consist of?
a. carboxylic acid group, ε carbon, nitro group, hydrogen atom
b. carbonyl group, α carbon, amino group, oxygen atom, distinctive R group
c. carboxylic acid group, δ carbon, Schiff base, hydrogen atom, distinctive R group
d. carboxylic acid group, α carbon, amino group, hydrogen atom, distinctive R group
e. alcohol group, α carbon, amino group, nitrogen atom, distinctive R group, hydrogen atom -
Answers d. carboxylic acid group, α carbon, amino group, hydrogen atom, distinctive R group
An example of closed symmetry is
a. actin filaments
b. antibodies
c. sickle cell hemoglobin filaments
d. microtubules - Answers b. antibodies
Which of the following is CORRECT, regarding Hb and Mb?
a. the R-state of Mb binds oxygen
b. the R-state of Hb binds oxygen
c. the T-state of Hb transports oxygen
d. the T-state of Mb transports oxygen
e. the R-state of Hb stores oxygen - Answers b. the R-state of Hb binds oxygen
What is the difference between β strands and loops?
a. Loops do not have a three-dimensional structure.
b. Loops have a greater molecular weight.
c. Loops do not have hydrogen bonds between side chains.
d. Loops do not have regular backbone phi and psi angles.
e. Loops do not have amino acid residues. - Answers d. Loops do not have regular backbone phi and
psi angles.
The allosteric effector, 2,3-BPG, makes
a. the O2 binding curve of Mb hyperbolic
b. the O2 binding curve of Hb-F sigmoidal
c. the O2 binding curve of Hb sigmoidal
d. the O2 binding curve of Mb sigmoidal
e. the binding of O2 to Hb stronger - Answers c. the O2 binding curve of Hb sigmoidal
What is a structure of the hemoglobin molecule?
a. α2 dimer
b. α2β2 tetramer
c. α1β2 trimer
d. β2 dimer
e. α2β1 trimer - Answers b. α2β2 tetramer
, What chemical is used to release the N-terminal amino acid of a polypeptide?
a. Iodoacetic acid
b. phenyl isothiocyanate
c. Sodium dodecyl sulfate
d. Cyanogen bromide
e. Beta mercaptoethanol - Answers b. phenyl isothiocyanate
What residues are hydrolyzed on their C-side during trypsin digestion?
a. Asn and Lys
b. Lys and Val
c. Ser and Met
d. Val and Ser
e. Arg and Lys - Answers e. Arg and Lys
Choose the order of the atoms that lie in the amide plane that links amino acids.
a. Cα, Cα2, Cα3, NH, CO
b. Cα, CO, NH, Cα3
c. Cα, Cα2, NH, CO
d. Cα3, CO, NH, Cα2
e. Cα, CO, NH, Cα2 - Answers e. Cα, CO, NH, Cα2
What method can be used to separate proteins by specific binding with other molecules?
a. gel-filtration chromatography
b. affinity chromatography
c. SDS-PAGE
d. ion-exchange chromatography
e. dialysis - Answers b. affinity chromatography
A good example of open symmetry is
a. chaperones
b. antibodies
c. hemoglobin
d. microtubules - Answers d. microtubules
What is the amino-terminal residue and what is the carboxyl-terminal residue in the sequence of
amino acids Gly-Tyr-Gly-Phe-Leu?
a. There are no N-terminal and C-terminal residues in the sequences of amino acids.
b. Leucine is N-terminal and glycine is C-terminal.
c. Phenylalanine is N-terminal and leucine is C-terminal.
d. Tyrosine is N-terminal and leucine is C-terminal.
e. Glycine is N-terminal and leucine is C-terminal. - Answers e. Glycine is N-terminal and leucine is C-
terminal.
In the womb, the fetus obtains oxygen from the mother through the placenta by
a. tighter binding of Hb F to 2,3-BPG
b. joined veins and capillaries, with Hb transporting O2 from the mother
c. tighter binding of Hb F to O2
d. weaker binding of Hb F to 2,3-BPG
e. weaker binding of Hb F to 2,3-BPG and tighter binding of Hb F to O2 - Answers e. weaker binding of
Hb F to 2,3-BPG and tighter binding of Hb F to O2
Which of the following about the influenza HA structure is TRUE?
a. The conformational change involves movement of the F8 helix.
b. The structure is monomeric, until the conformational change is triggered.
c. The trimer uses a banana-shape to bind and bend the membrane.
d. The conformational change results in relocation of the fusion peptides into the target membrane.
e. The trimer is held together with a collagen-like triple helix. - Answers d. The conformational change
results in relocation of the fusion peptides into the target membrane.
_____ is added prior to gel electrophoresis to denature proteins.
a. Polyacrylamide
b. Cyanogen bromide
c. SDS
d. β-mercaptoethanol
e. Coomassie Blue - Answers c. SDS
