BIOLOGY 305 Quiz 1

An evolutionary advancement occurred when organisms became multicellular and acquired a division of labor Filopodia contains receptors that sense the environment and Single-celled organisms have limited number of biomolecular machines within them When a cell is anchorage-dependent for growth, it can then go through the cell cycle when it is suspended Contact inhibition is a subset of What limits the growth of tumor When 1 mm tissue fragment is placed in the flask to loosen the cell in the tissue, what were the components of the medium What happens in the primary culture Explain two methods of making a cell line in detail What are limitations of cell culture technology Loss of cell wall Primitive protoprokaryote likely acquire motility before it lost the cell wall Peroxisomes likely orginiated from endosymbiosis Peroxisomes increased the fitness of primitive protoprokaryote by removing toxic oxygen in ancient earth Endomembrane system supposedly resulted in the formation of nuclear envelope, endoplasmic reticulum, golgi apparatus and primary lysosomes The thickness of the membrane is Phospholipid, the fatty acid chains are linked to the glycerol by ester bonds The hydrophilic head group is hydrophilic because it can hydrogen bond An organic precipitation works because the solvent, , disrupts ……………… of the solute. Homogenization buffer can be identical to an intracellular buffer A salt wash of membranes is used to remove cytoskeleton (removes peripheral membrane) Cell wash removes cytoskeleton Actin Filaments: Actin filaments will The number of amino acids in each actin monomer is Hydrolysis of ATP to ADP Actin filaments associate with a nucleotide triphosphate Treadmiling of actin filaments only occur in a test tube