ASCP CG CERTIFICATION PRACTICE QUESTIONS AND CORRECT DETAILED
ANSWERS (VERIFIED ANSWERS) | ASSURED SUCCESS
What is the best agent to use against mycoplasma? -
Answer-BM cyclin - a combination of two antibiotics (tiamulin and minocycline) that effecticely targets
mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: -
Answer-comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: -
Answer-Proteolytic enzymes - break down tissues into single- cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters following incubation
with: -
Answer-Colcemid - mitotic spindle poison that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: -
Answer-Logged in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
Answer-T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is the MOST
important to document? -
Answer-Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? -
Answer-Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? -
Answer-Decidua (maternal tissue)
,When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around the
mets, what is the most likely cause? -
Answer-innappropriate slide drying time
, What will help prevent microbial contamination and cross contamination of cultures? -
Answer-Asceptic technique
When there are not enough mets to complete the study of a patient/case, what needs to be done next?
-
Answer-prepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out and sparse, what
could help fix the issue? -
Answer-Increading the drying time by using warm slides
What is true of an inverted microscope? -
Answer-the objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: -
Answer-DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide spread
eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST important data to
accompany the specimen? -
Answer-Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? -
Answer-Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
ANSWERS (VERIFIED ANSWERS) | ASSURED SUCCESS
What is the best agent to use against mycoplasma? -
Answer-BM cyclin - a combination of two antibiotics (tiamulin and minocycline) that effecticely targets
mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: -
Answer-comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: -
Answer-Proteolytic enzymes - break down tissues into single- cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters following incubation
with: -
Answer-Colcemid - mitotic spindle poison that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: -
Answer-Logged in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
Answer-T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is the MOST
important to document? -
Answer-Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? -
Answer-Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? -
Answer-Decidua (maternal tissue)
,When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around the
mets, what is the most likely cause? -
Answer-innappropriate slide drying time
, What will help prevent microbial contamination and cross contamination of cultures? -
Answer-Asceptic technique
When there are not enough mets to complete the study of a patient/case, what needs to be done next?
-
Answer-prepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out and sparse, what
could help fix the issue? -
Answer-Increading the drying time by using warm slides
What is true of an inverted microscope? -
Answer-the objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: -
Answer-DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide spread
eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST important data to
accompany the specimen? -
Answer-Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? -
Answer-Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
