MRO UPDATED ACTUAL EXAM #2 QUESTIONS
WITH VERIFIED ANSWERS
Federal Chain of Custody Form (CCF):
What constitutes a fatal flaw? (40.199)
1. No CCF
2. No specimen submitted with the CCF
3. No printed collector's name and no collector's signature
4. 2 separate collections using 1 CCF
5. Specimen ID numbers on bottle and form don't match
6. Broken seal or evidence of tampering (Split specimen cannot be re-designated)
7. Leakage, insufficient amount of urine (Split cannot be re-designated)
Federal Chain of Custody Form (CCF):
What are considered minor or correctable flaws? (40.203)
1. Employee's signature is omitted from the certification statement (unless refusal to sign in
"Remarks" line)
2. Certifying scientist's signature omitted -(+), adulterated, substituted or invalid test
3. Non-federal form or expired CCF used, procedure must be correct.
DOT Collection Procedures:
What are the basic steps of the collection process? (40.61-40.73)
1. Photo ID for employee and collector
2. Explain procedure
3. Alcohol testing first
4. Remove outer clothing
5. Wash hands
6. Kit selection
7. Direct to the Bathroom
8. Pay Attention
DOT Collection Procedures:
When must an observed collection be ordered by the collector? (40.67)
1. Directed by DER
2. Attempt to tamper
3. Temperature on original out of range
4. Tampered with
5. Same gender
DOT Collection Procedures:
,When must an observed collection be ordered by the employer?
a. Lab states test invalid and MRO states no medical explanation;
b. Original (+), adulterated or substituted and split cannot be performed
c. Return to Duty
d. Neg-dilute with Cr > 2mg/dL but < 5mg/dL
DOT Collection Procedures:
When must an observed collection be ordered by the MRO?
Shy Bladder:
What are DOT’s regulations regarding shy bladder (Insufficient volume)? (40.193) Less
than 45ML
1) Discard specimen unless temp out of range or shows signs of tampering and then note in
"remarks"
2) Urge to drink up to 40oz in up to 3 hours
3) If Employee refuses or leaves site discontinue and note on remark line
4) Discontinue after 3 hours and note on Remarks line
Shy Bladder:
Who performs the medical evaluation for shy bladder? (40.193)
· A medical doctor within 5 days
Shy Bladder:
What does the medical evaluation for shy bladder consist of? (40.193)
· To Determine the pre-existing physiologic or mental condition, blood chemistries, kidney
function. NO Dehydration or anxiety
Shy Bladder:
What are DOT’s regulations regarding long-term disabilities that cause the donor to be
unable to provide sufficient urine? [Note the specific regulations regarding insufficient
volume and long-term disabilities area applied for pre-employment, return-to-work, and
follow-up tests only, not for random testing.] (40.195)
a) Determine if a drug user
b) Provide an alternative test
Laboratory Procedures:
What is the purpose of the immunoassay tests?
Immunoassay are used to screen urine drug tests.
, Laboratory Procedures:
What are the cutoff levels used in federally regulated testing Immunoassay?
THCA
50ng/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated testing Immunoassay?
Cocaine
Benzoylecgonine 150nG/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated
testing Immnoassay ?Codeine/Morphine
330ng/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated testing Immunoassay?
Hydrocodone/Hydromorphone
100ng/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated testing Immunoassay?
6-AM
10ng/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated
testing Immunoassay? Amphetamine/metamphetamine
500ng/mL
What are the cutoff levels used in federally regulated testing Immunoassay? Laboratory
Procedures:
500ng/mL
Laboratory Procedures:
What is the purpose of the GC/MS confirmation?
WITH VERIFIED ANSWERS
Federal Chain of Custody Form (CCF):
What constitutes a fatal flaw? (40.199)
1. No CCF
2. No specimen submitted with the CCF
3. No printed collector's name and no collector's signature
4. 2 separate collections using 1 CCF
5. Specimen ID numbers on bottle and form don't match
6. Broken seal or evidence of tampering (Split specimen cannot be re-designated)
7. Leakage, insufficient amount of urine (Split cannot be re-designated)
Federal Chain of Custody Form (CCF):
What are considered minor or correctable flaws? (40.203)
1. Employee's signature is omitted from the certification statement (unless refusal to sign in
"Remarks" line)
2. Certifying scientist's signature omitted -(+), adulterated, substituted or invalid test
3. Non-federal form or expired CCF used, procedure must be correct.
DOT Collection Procedures:
What are the basic steps of the collection process? (40.61-40.73)
1. Photo ID for employee and collector
2. Explain procedure
3. Alcohol testing first
4. Remove outer clothing
5. Wash hands
6. Kit selection
7. Direct to the Bathroom
8. Pay Attention
DOT Collection Procedures:
When must an observed collection be ordered by the collector? (40.67)
1. Directed by DER
2. Attempt to tamper
3. Temperature on original out of range
4. Tampered with
5. Same gender
DOT Collection Procedures:
,When must an observed collection be ordered by the employer?
a. Lab states test invalid and MRO states no medical explanation;
b. Original (+), adulterated or substituted and split cannot be performed
c. Return to Duty
d. Neg-dilute with Cr > 2mg/dL but < 5mg/dL
DOT Collection Procedures:
When must an observed collection be ordered by the MRO?
Shy Bladder:
What are DOT’s regulations regarding shy bladder (Insufficient volume)? (40.193) Less
than 45ML
1) Discard specimen unless temp out of range or shows signs of tampering and then note in
"remarks"
2) Urge to drink up to 40oz in up to 3 hours
3) If Employee refuses or leaves site discontinue and note on remark line
4) Discontinue after 3 hours and note on Remarks line
Shy Bladder:
Who performs the medical evaluation for shy bladder? (40.193)
· A medical doctor within 5 days
Shy Bladder:
What does the medical evaluation for shy bladder consist of? (40.193)
· To Determine the pre-existing physiologic or mental condition, blood chemistries, kidney
function. NO Dehydration or anxiety
Shy Bladder:
What are DOT’s regulations regarding long-term disabilities that cause the donor to be
unable to provide sufficient urine? [Note the specific regulations regarding insufficient
volume and long-term disabilities area applied for pre-employment, return-to-work, and
follow-up tests only, not for random testing.] (40.195)
a) Determine if a drug user
b) Provide an alternative test
Laboratory Procedures:
What is the purpose of the immunoassay tests?
Immunoassay are used to screen urine drug tests.
, Laboratory Procedures:
What are the cutoff levels used in federally regulated testing Immunoassay?
THCA
50ng/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated testing Immunoassay?
Cocaine
Benzoylecgonine 150nG/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated
testing Immnoassay ?Codeine/Morphine
330ng/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated testing Immunoassay?
Hydrocodone/Hydromorphone
100ng/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated testing Immunoassay?
6-AM
10ng/mL
Laboratory Procedures:
What are the cutoff levels used in federally regulated
testing Immunoassay? Amphetamine/metamphetamine
500ng/mL
What are the cutoff levels used in federally regulated testing Immunoassay? Laboratory
Procedures:
500ng/mL
Laboratory Procedures:
What is the purpose of the GC/MS confirmation?
