AAB MT Chemistry Exam with precise detailed ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
solutions
Which of the four elements is proteins differentiates this class substances from
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
carbohydrates and lipids? ||\\//|| ||\\//||
A. carbon
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B. hydrogen
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C. nitrogen
||\\//|| ||\\//||
D. oxygen - correct answer✔✔C. Nitrogen
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Which of the proteins is soluble in water?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. albumin
||\\//||
B. alpha-globulins
||\\//||
C. beta-globulins
||\\//|| ||\\//||
D. gamma-globulins - correct answer✔✔A. Albumin
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
How are amino acids joined together to form a chainlike structure?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. disulfide bonding
||\\//|| ||\\//||
B. hydrogen bonding
||\\//|| ||\\//||
C. covalent bonding
||\\//|| ||\\//|| ||\\//||
D. peptide bonding - correct answer✔✔D. Peptide bonding
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
,Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
treatment. What does denaturation of proteins refer to? ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. amino acid substitution
||\\//|| ||\\//|| ||\\//|| ||\\//||
B. addition of a prosthetic group
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
C. alteration in tertiary structure
||\\//|| ||\\//|| ||\\//|| ||\\//||
D. increase in solubility - correct answer✔✔C. Alteration in tertiary structure
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Which aminoacidopathy is NOT caused by an enzyme deficiency?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. maple syrup substitution
||\\//|| ||\\//|| ||\\//|| ||\\//||
B. phenylketonuria
||\\//|| ||\\//||
C. alkaptonuria
||\\//|| ||\\//||
D. cystinuria - correct answer✔✔D. Cystinuria
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
How many immunoglobulin classes are currently recognized?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. two||\\//|| ||\\//||
B. three
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C. four
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D. five - correct answer✔✔D. Five
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Which reagent is employed in the serum protein determination?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. biuret
||\\//|| ||\\//||
B. molybdenum blue
||\\//|| ||\\//|| ||\\//||
,C. ferricferrocyanide
||\\//|| ||\\//||
D. diacetyl monoxime - correct answer✔✔A. Biuret
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Why should hemolyzed serum not be used for total protein analysis?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. hemoglobin is absorbed at the same wavelength as protein in the reagent
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
B. there are elevated protein amounts in red blood cells as compared to serum
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
C. hemoglobin reacts with biuret reagent
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
D. the value will be falsely lowered - correct answer✔✔A. Hemoglobin is absorbed at the
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
same wavelength as protein in the reagent
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Which of the following is NOT true of the biuret reaction? ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. follows Beer's Law for a reasonable range of protein
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
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B. depends on the presence of at least two peptide linkages
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
C. is relatively free from interference by lipids and hemoglobin
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
D. is insensitive to the low protein levels of urine - correct answer✔✔C. is relatively free
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
from interference by lipids and hemoglobin
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Why is bromcresol purple the preferred indicator for albumin dye binding techniques?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
, A. it lends itself to manual and automated procedures
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
||\\//||
B. it never bind with alpha or beta globulins
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
C. it can be employed with both serum and plasma
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
||\\//||
D. there is less interference from pigment(s) - correct answer✔✔D. there is less interference
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
from pigment(s)
||\\//|| ||\\//||
What is the most anodic protein on electrophoresis at pH 8.6?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. albumin ||\\//|| ||\\//||
B. alpha-globulins
||\\//|| ||\\//||
C. beta-globulins
||\\//|| ||\\//||
D. gamma-globulins - correct answer✔✔A. albumin
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
The total protein is 7.2. The serum protein results are as follows:
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Albumin 53% 3.8 g/dL ||\\//|| ||\\//|| ||\\//||
Alpha-1 3.6% 0.3 g/dL ||\\//|| ||\\//|| ||\\//||
Alpha-2 11% 0.8 g/dL ||\\//|| ||\\//|| ||\\//||
Betta 17.6% 1.2 g/dL ||\\//|| ||\\//|| ||\\//||
Gamma 14.8% 1.1 g/dL ||\\//|| ||\\//|| ||\\//||
What is the A/G ratio? ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. 3.4 ||\\//|| ||\\//||
B. 1.6 ||\\//||
solutions
Which of the four elements is proteins differentiates this class substances from
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
carbohydrates and lipids? ||\\//|| ||\\//||
A. carbon
||\\//||
B. hydrogen
||\\//||
C. nitrogen
||\\//|| ||\\//||
D. oxygen - correct answer✔✔C. Nitrogen
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Which of the proteins is soluble in water?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. albumin
||\\//||
B. alpha-globulins
||\\//||
C. beta-globulins
||\\//|| ||\\//||
D. gamma-globulins - correct answer✔✔A. Albumin
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
How are amino acids joined together to form a chainlike structure?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. disulfide bonding
||\\//|| ||\\//||
B. hydrogen bonding
||\\//|| ||\\//||
C. covalent bonding
||\\//|| ||\\//|| ||\\//||
D. peptide bonding - correct answer✔✔D. Peptide bonding
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
,Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
treatment. What does denaturation of proteins refer to? ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. amino acid substitution
||\\//|| ||\\//|| ||\\//|| ||\\//||
B. addition of a prosthetic group
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
C. alteration in tertiary structure
||\\//|| ||\\//|| ||\\//|| ||\\//||
D. increase in solubility - correct answer✔✔C. Alteration in tertiary structure
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Which aminoacidopathy is NOT caused by an enzyme deficiency?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. maple syrup substitution
||\\//|| ||\\//|| ||\\//|| ||\\//||
B. phenylketonuria
||\\//|| ||\\//||
C. alkaptonuria
||\\//|| ||\\//||
D. cystinuria - correct answer✔✔D. Cystinuria
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
How many immunoglobulin classes are currently recognized?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. two||\\//|| ||\\//||
B. three
||\\//|| ||\\//||
C. four
||\\//|| ||\\//||
D. five - correct answer✔✔D. Five
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Which reagent is employed in the serum protein determination?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. biuret
||\\//|| ||\\//||
B. molybdenum blue
||\\//|| ||\\//|| ||\\//||
,C. ferricferrocyanide
||\\//|| ||\\//||
D. diacetyl monoxime - correct answer✔✔A. Biuret
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Why should hemolyzed serum not be used for total protein analysis?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. hemoglobin is absorbed at the same wavelength as protein in the reagent
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
B. there are elevated protein amounts in red blood cells as compared to serum
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
C. hemoglobin reacts with biuret reagent
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
D. the value will be falsely lowered - correct answer✔✔A. Hemoglobin is absorbed at the
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
same wavelength as protein in the reagent
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Which of the following is NOT true of the biuret reaction? ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. follows Beer's Law for a reasonable range of protein
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
||\\//||
B. depends on the presence of at least two peptide linkages
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
C. is relatively free from interference by lipids and hemoglobin
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
D. is insensitive to the low protein levels of urine - correct answer✔✔C. is relatively free
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
from interference by lipids and hemoglobin
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Why is bromcresol purple the preferred indicator for albumin dye binding techniques?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
, A. it lends itself to manual and automated procedures
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
||\\//||
B. it never bind with alpha or beta globulins
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
C. it can be employed with both serum and plasma
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
||\\//||
D. there is less interference from pigment(s) - correct answer✔✔D. there is less interference
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
from pigment(s)
||\\//|| ||\\//||
What is the most anodic protein on electrophoresis at pH 8.6?
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. albumin ||\\//|| ||\\//||
B. alpha-globulins
||\\//|| ||\\//||
C. beta-globulins
||\\//|| ||\\//||
D. gamma-globulins - correct answer✔✔A. albumin
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
The total protein is 7.2. The serum protein results are as follows:
||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//|| ||\\//||
Albumin 53% 3.8 g/dL ||\\//|| ||\\//|| ||\\//||
Alpha-1 3.6% 0.3 g/dL ||\\//|| ||\\//|| ||\\//||
Alpha-2 11% 0.8 g/dL ||\\//|| ||\\//|| ||\\//||
Betta 17.6% 1.2 g/dL ||\\//|| ||\\//|| ||\\//||
Gamma 14.8% 1.1 g/dL ||\\//|| ||\\//|| ||\\//||
What is the A/G ratio? ||\\//|| ||\\//|| ||\\//|| ||\\//||
A. 3.4 ||\\//|| ||\\//||
B. 1.6 ||\\//||
