Brock Biology of Microorganisms 15e (Madigan et al.) — Chapter 12 Test Bank

Brock Biology of Microorganisms, 15eBrock
(Madigan
Biology
et al.)
of Microorganisms,
Chapter 12 Test15e
Bank
(Madigan et al.) Chapter 12 Test Bank 2026/02/16




Chapter 12 - test bank


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Brock Biology of Microorganisms, 15e (Madigan et al.)
Chapter 12 Biotechnology and Synthetic Biology

12.1 Multiple Choice Questions

1) If a foreign gene is cloned into an expression host, it is important that the host itself
A) not produce the protein being studied.
B) produce the protein in larger amounts than the vector.
C) repress the genetic expression being studied.
D) produce signal proteins to tag the host protein.
Answer: A
Bloom’s Taxonomy: 1-2: Remembering/Understanding
Chapter Section: 12.3

2) Cells that have "insertional inactivation" of the lacZ gene are
A) blue.
B) white.
C) yellow.
D) fluorescent green.
Answer: B
Bloom’s Taxonomy: 1-2: Remembering/Understanding
Chapter Section: 12.2

3) Cosmids are a type of
A) bacterial artificial chromosomes (BACs).
B) cloning vector.
C) heat stable polymerase.
D) RNA/DNA hybrid.
Answer: B
Bloom’s Taxonomy: 3-4: Applying/Analyzing
Chapter Section: 12.2

4) Expression vectors are designed to ensure that can be efficiently .
A) mRNA / transcribed
B) DNA / transcribed
C) mRNA / translated
D) DNA / translated
Answer: A
Bloom’s Taxonomy: 1-2: Remembering/Understanding
Chapter Section: 12.3




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5) A(n) gene is a gene that encodes a protein that is easy to detect and assay.
A) encoder
B) translational
C) reporter
D) recorder
Answer: C
Bloom’s Taxonomy: 1-2: Remembering/Understanding
Chapter Section: 12.5

6) One of the more formidable obstacles to mammalian gene cloning is the presence of
A) introns.
B) exons.
C) repressors.
D) integrators.
Answer: A
Bloom’s Taxonomy: 1-2: Remembering/Understanding
Chapter Section: 12.3

7) What is the most important advantage of Pfu polymerase over Taq polymerase?
A) Unlike Taq polymerase, Pfu polymerase functions well at relatively high temperatures.
B) It is from a bacterium, not an archaean, so it is more effective for replicating eukaryotic DNA.
C) Pfu polymerase removes the need for primers during PCR.
D) Unlike Taq polymerase, Pfu polymerase has proofreading activity.
Answer: D
Bloom’s Taxonomy: 1-2: Remembering/Understanding
Chapter Section: 12.1

8) What is the difference between PCR and RT-PCR?
A) Only PCR makes many copies of DNA rapidly.
B) RT-PCR uses an RNA template whereas PCR uses a DNA template.
C) Only PCR produces cDNA.
D) PCR uses a single stranded template whereas RT-PCR uses a double-stranded template.
Answer: C
Bloom’s Taxonomy: 1-2: Remembering/Understanding
Chapter Section: 12.1

9) The genes encoding green fluorescent protein (GFP) and β-galactosidase are typically used in
cloning as
A) transcription regulators.
B) global control genes.
C) promoter sequences.
D) reporter genes.
Answer: D
Bloom’s Taxonomy: 3-4: Applying/Analyzing
Chapter Section: 12.4



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