BIOD 171 MICROBIOLOGY EXAM QUESTIONS AND CORRECT VERIFIED ANSWERS

BIOD 171 MICROBIOLOGY EXAM QUESTIONS AND CORRECT VERIFIED ANSWERS Agar Plate Derived from: - ANSWER Derived from: Seaweed (Red Algae) Blood Agar Selective: Differential: Enriched: Special Culture: Hemolysis Properties: 1) 2) 3) – ANSWER Selective: No Differential: Yes Enriched: Yes Special Culture: Streptococcus Hemolysis Properties: 1) Alpha: Brown 2) Beta: Clear 3) Gamma: Red Chocolate Agar Selective: Differential: Enriched: - ANSWER Selective: No Differential: No Enriched: Yes Columbia CNA Agar Selective: Differential: Enriched: Special cultures: - ANSWER Selective: Yes Differential: Yes Enriched: Yes Special cultures: Gram-Positive Microbes Culture Media: 1) 2) 3) 4) - ANSWER1) Growth Media: General growth 2) Selective Media: Grows specific bacteria while inhibiting other 3) Differential Media: Distinguished between 2 microbes 4) Enriched Media: Grows fastidious organisms Culturing Microorganisms: 1) 2) 3) 4) - ANSWER1) Inoculum: Sample 2) Medium: Broth or solid media 3) Culture: Microbes grown 4) Colonies: Cluster of cells arising from 1 parent cell Eosin Methylene Blue (EMB) - ANSWERSelective and Differential Growth Media Categories Defined Media: Undefined Media: - ANSWER Defined Media: Known quantities Undefined Media: Unknown ratios Growth Requirements of Bacteria 1) 2) 3) - ANSWER1) Nutrients 2) Temperature 3) pH Lysogeny Broth (LB) Agar Selective: Differential: Enriched: Special Culture: - ANSWER Selective: No Differential: No Enriched: No Special Culture: E. coli MacConkey Agar Selective: Differential: Enriched: Common Growth: - ANSWER Common Growth: E. coli O157:H7 Mannitol Salt Agar Common Growth: - ANSWER Selective and Differential Common Growth: Gram-Positive; differentiates staphylococci NUTRIENTS: Microbial Categories (Carbon and Energy): 1) 2) 3) 4) - ANSWER1) Photoautotrophs- Light energy and CO2 2) Photoheterotrophs- Light energy and organic compounds 3) Chemoautotrophs- Chemical energy and CO2 4) Chemoheterotrophs- Chemical energy and organic compounds NUTRIENTS: Microbial Categories (Oxygen): 1) 2) 3) - ANSWER1) Obligate Aerobes: Require O2 2) Obligate Anaerobes: Killed by O2 3) Facultative Anaerobes: Grow better with O2, but can live without it Other Nutrients (Not for Classification): 1) 2) 3) - ANSWER1) Nitrogen 2) Trace elements 3) Growth factors pH: Microbial Categories: 1) 2) 3) - ANSWER1) Acidophiles 2) Neutrophiles 3) Alkalinophiles TEMPERATURE: Microbial Categories