HEMATOLOGY MUST-KNOW QUESTIONS AND
ANSWERS
STUDY MATERIALS WITH Q&A 2026/2027 LATEST
Skin puncture - CORRECT ANSWER>>>>1. Fingertips
2 . Earlobe: less admixture w/ tissue juice, less pain, less free nerve endings 3.
Lateral portion of the plantar surface of the foot: <1 year old Difference from
venous specimen:
WBCs ◻ Hgb, Hct,
RBCs, platelet
Venipuncture - CORRECT ANSWER>>>>Veins in the arms (antecubital region):
1. Median cubital = preferred, most stable
2. Cephalic (lateral)
3. Basilic (medial)
Common gauge (needle) - CORRECT ANSWER>>>>19, 20, 21
Routine: 20g
Common length of needle - CORRECT ANSWER>>>>1-1.5 inches
Color coded hub (gauge) - CORRECT ANSWER>>>>18 = pink
2 1 = green
2 2 = gray
2 3 = blue/light blue/turquoise
1|Page
,Angle - CORRECT ANSWER>>>>Venipuncture: 150
BB: 450 ◻ 10-200 once in the skin
BP cuff as tourniquet - CORRECT ANSWER>>>>40-60 mmHg
Reassure the patient - CORRECT ANSWER>>>>Crying = ◻ cell count
Position the patient - CORRECT ANSWER>>>>Lying down = hemodilution (
PCV by 8%, ◻ WBC) ◻
Lying up = hemoconcentration
IV line - CORRECT ANSWER>>>>Collect on the other arm
If both arms: Stop IV for 2mins
= Collect blood below the IV line
= Appropriate for all analytes except glucose and phosphorus
Hematopoiesis - CORRECT ANSWER>>>>Cellular formation, proliferation,
differentiation and maturation of blood cells
Mesoblastic period - CORRECT ANSWER>>>>19th day of gestation
Yolk salk = Erythropoiesis Embryonic hemoglobins:
a. Gower 1 = Zeta2 + Epsilon2
b. Portland = Zeta2 + Gamma2
c. Gower 2 = Alpha2 + Epsilon2
2|Page
, Hematology - CORRECT ANSWER>>>>Greek:
- Haima = Blood
- Logos = Study/science
EDTA (Lavender top) - CORRECT ANSWER>>>>Chelates calcium
Inversion: 8x
Anticoagulant of choice for hematology cell counts and cell morphology Blood
smear: prepare w/in 2 hrs
Preferred anticoagulant for platelet count:
= In some patients w/ EDTA anticoagulated blood - platelet satellitism = Platelet
satellitism: platelets adhere to neutrophils
♫ Effect to automated platelet count Decreased
♫ Remedy: Repeat platelet count◻ using◻ ◻citrate (Rodak: Platelet count x 1.1)
◻
EDTA = Shrinkage of cells = Hct = ESR Not for
coagulation tests:
= Inhibits fibrinogen-thrombin reaction
= Factor V is not stable in EDTA
Modified Westergren ESR (Black top tube) - CORRECT ANSWER>>>>2mL
EDTA + 0.5mL NSS/Citrate
Ratio = 1:4 (Anticoagulant-to-Blood)
Citrate (Light blue top tube) - CORRECT ANSWER>>>>For coagulation and
platelet studies
3|Page
ANSWERS
STUDY MATERIALS WITH Q&A 2026/2027 LATEST
Skin puncture - CORRECT ANSWER>>>>1. Fingertips
2 . Earlobe: less admixture w/ tissue juice, less pain, less free nerve endings 3.
Lateral portion of the plantar surface of the foot: <1 year old Difference from
venous specimen:
WBCs ◻ Hgb, Hct,
RBCs, platelet
Venipuncture - CORRECT ANSWER>>>>Veins in the arms (antecubital region):
1. Median cubital = preferred, most stable
2. Cephalic (lateral)
3. Basilic (medial)
Common gauge (needle) - CORRECT ANSWER>>>>19, 20, 21
Routine: 20g
Common length of needle - CORRECT ANSWER>>>>1-1.5 inches
Color coded hub (gauge) - CORRECT ANSWER>>>>18 = pink
2 1 = green
2 2 = gray
2 3 = blue/light blue/turquoise
1|Page
,Angle - CORRECT ANSWER>>>>Venipuncture: 150
BB: 450 ◻ 10-200 once in the skin
BP cuff as tourniquet - CORRECT ANSWER>>>>40-60 mmHg
Reassure the patient - CORRECT ANSWER>>>>Crying = ◻ cell count
Position the patient - CORRECT ANSWER>>>>Lying down = hemodilution (
PCV by 8%, ◻ WBC) ◻
Lying up = hemoconcentration
IV line - CORRECT ANSWER>>>>Collect on the other arm
If both arms: Stop IV for 2mins
= Collect blood below the IV line
= Appropriate for all analytes except glucose and phosphorus
Hematopoiesis - CORRECT ANSWER>>>>Cellular formation, proliferation,
differentiation and maturation of blood cells
Mesoblastic period - CORRECT ANSWER>>>>19th day of gestation
Yolk salk = Erythropoiesis Embryonic hemoglobins:
a. Gower 1 = Zeta2 + Epsilon2
b. Portland = Zeta2 + Gamma2
c. Gower 2 = Alpha2 + Epsilon2
2|Page
, Hematology - CORRECT ANSWER>>>>Greek:
- Haima = Blood
- Logos = Study/science
EDTA (Lavender top) - CORRECT ANSWER>>>>Chelates calcium
Inversion: 8x
Anticoagulant of choice for hematology cell counts and cell morphology Blood
smear: prepare w/in 2 hrs
Preferred anticoagulant for platelet count:
= In some patients w/ EDTA anticoagulated blood - platelet satellitism = Platelet
satellitism: platelets adhere to neutrophils
♫ Effect to automated platelet count Decreased
♫ Remedy: Repeat platelet count◻ using◻ ◻citrate (Rodak: Platelet count x 1.1)
◻
EDTA = Shrinkage of cells = Hct = ESR Not for
coagulation tests:
= Inhibits fibrinogen-thrombin reaction
= Factor V is not stable in EDTA
Modified Westergren ESR (Black top tube) - CORRECT ANSWER>>>>2mL
EDTA + 0.5mL NSS/Citrate
Ratio = 1:4 (Anticoagulant-to-Blood)
Citrate (Light blue top tube) - CORRECT ANSWER>>>>For coagulation and
platelet studies
3|Page
