MOLECULAR CELL BIOLOGY CHAPTER 18
LATEST EXAM PAPER SOLVED QUESTIONS AND
EXPERT VERIFIED ANSWERS 2026 EDITION
◉What are the two conditions cultured cells can be grown in? . Answer:
Serum
Serum Free
◉What is the most important thing to consider when performing
experiments on cultured cells? . Answer: Minimize Microbial
Contamination
EXTREMELY IMPORTANT
◉What are two ways we can minimize microbial contamination? .
Answer: Bio-safety Cabinet
Antibiotics in the serum
◉What is a cell line? . Answer: Cells in a culture that are derived from
the same original cell (often cancer cells for their continuous division)
, ◉What is the importance of keeping calcium out of the culture when
creating a cell line? . Answer: Calcium allows for cell-cell adhesion - it
is important to prevent this so they can grow individually
◉What is an example of a primary culture for Euk? . Answer: An
Embryo
◉What are some aspects as to why we use cancer cells for creating a
cell line? (4) . Answer: Do not senesce (deteriorate with age)
Dont require adherence surface
Proliferate in high density (divide a lot)
There is no hay flick limit!
◉How can cell lines be stored indefinitely? . Answer: In liquid nitrogen
◉What is a heterokaryon? . Answer: A cell with two or more distinct
Nuclei
◉What are the steps of creating Hybrid cell lines? (3) . Answer: Human
fibroblast and mouse tumour cell are fused to form heterokaryons
The selective medium allows only heterokaryons to proliferate
Hybrid cells are formed!
LATEST EXAM PAPER SOLVED QUESTIONS AND
EXPERT VERIFIED ANSWERS 2026 EDITION
◉What are the two conditions cultured cells can be grown in? . Answer:
Serum
Serum Free
◉What is the most important thing to consider when performing
experiments on cultured cells? . Answer: Minimize Microbial
Contamination
EXTREMELY IMPORTANT
◉What are two ways we can minimize microbial contamination? .
Answer: Bio-safety Cabinet
Antibiotics in the serum
◉What is a cell line? . Answer: Cells in a culture that are derived from
the same original cell (often cancer cells for their continuous division)
, ◉What is the importance of keeping calcium out of the culture when
creating a cell line? . Answer: Calcium allows for cell-cell adhesion - it
is important to prevent this so they can grow individually
◉What is an example of a primary culture for Euk? . Answer: An
Embryo
◉What are some aspects as to why we use cancer cells for creating a
cell line? (4) . Answer: Do not senesce (deteriorate with age)
Dont require adherence surface
Proliferate in high density (divide a lot)
There is no hay flick limit!
◉How can cell lines be stored indefinitely? . Answer: In liquid nitrogen
◉What is a heterokaryon? . Answer: A cell with two or more distinct
Nuclei
◉What are the steps of creating Hybrid cell lines? (3) . Answer: Human
fibroblast and mouse tumour cell are fused to form heterokaryons
The selective medium allows only heterokaryons to proliferate
Hybrid cells are formed!
