MIP 300 EXAM 1 CSU QUESTIONS AND
ANSWERS GRADED A+ 2025/2026
What is required by viruses? - ANS they must be inside a cell to replicate and are called
obligate intracellular parasites
What are 75% of the 80 new diseases discovered between 1980 and 2007? - ANS RNA viruses
What does it mean to be alive? - ANS It means to have a metabolism, to use or produce
energy, and to replicate
From the meaning behind "being alive", what all do viruses do? - ANS they replicate,
however they do not have a metabolism and they do not use or produce energy
What are the 3 domains used to classify microbes? - ANS 1. Bacteria
2. Archaea
3. Eukarya
Are there more humans or microbes on Earth? - ANS microbes
In the 3 domains used to classify microbes, how do the domains differ? - ANS they differ by
having different ribosomal RNA sequences
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,What microbes do not fall in the 3 domains of classifying microbes? - ANS Viruses and Prions
Which of the following are eukaryotic microbes?
A. Viruses
B. Bacteria
C. Animals
D. Yeast
E. C and D - ANS D, not A or B because they are not eukaryotic, and not C because animals
are not microbes
Viruses are classified in which domain?
A. Bacteria
B. Archaea
C. Eukarya
D. Viralae
E. None of the above - ANS E, because D does not exist as a domain and viruses do not fall in
A, B, or C
What is the magnification on a microscope? - ANS the objective lens x the eyepiece
What is the objective lens on a microscope? - ANS the specific magnifications - 10X, 40X,
100X - with X meaning the total magnification will be multiplied by 10 so for example 10X total
magnification will be 100
What is the eyepiece on a microscope? - ANS the thing you look through to view the microbe
which automatically magnifies by 10X
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,What is resolution when referring to a microscope? - ANS the ability of the lens to distinguish
between objects that are close together, also called the resolving power
What is the best light microscope resolution? - ANS 0.2 um
What is the diameter of a human cell and from this diameter could you see a human cell using a
light microscope? - ANS 10-100 um, yes you could see this on a light microscope
What is the diameter of a bacterial cell and from this diameter could you see it using a light
microscope? - ANS 1 um, yes you could see this on a light microscope, however only to see
shape, size, and arrangement of bacteria
What is the diameter of a virus and from this diameter could you see it using a light
microscope? - ANS 0.01 - 0.1 um, viruses are too small to see with a light microscope
What are the general sizes from biggest to largest of human cells, bacterial cells, and viruses? -
ANS Human Cells > Bacterial Cells > Viruses
What do you see when looking through the eyepiece of a bright field microscope? - ANS the
backfield will be bright and white and the specimen you're studying will be the color you stain
it, otherwise the specimen will be clear
What is the total magnification? - ANS objective lens x 10
What does a bright field microscope require? - ANS it requires heat-fix and stained bacteria
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, What does it mean to heat fix a smear? - ANS it means to pass the slide through a flame
which kills them and makes the bacteria stick to the slide
What do you see when looking through the eyepiece of a dark field microscope? - ANS light is
refracted by the organism, so the organism is bright and the backfield is dark
What does a dark field microscope require? - ANS nothing, heat-fix and stain is not required
so you can look at live organisms
What do you see when looking through the eyepiece of a phase contrast microscope? -
ANS it converts slight differences in refractive indexes into easily detected variations in light
intensity so you can look at live cells intracellular structures or intracellular processes such as
phagocytosis
How does a fluorescent microscope work? - ANS it uses a UV light to excite fluorescent
molecules such as green fluorescent protein or fluorochrome labeled antibodies
What is a way that a fluorescent microscope can be used not involving antibodies? - ANS The
organism takes up fluorescent dye directly such as green fluorescent protein and the dead cells
take up red dye
What is a way that a fluorescent microscope can be used involving antibodies? - ANS bacteria
can be linked to antibodies which are linked to fluorochrome which is a fluorescent molecule
Describe the resolution difference between a light microscope and an electron microscope? -
ANS an electron microscope resolution is 1000x better than a light microscope (5 x 10^-4 um)
How is an electron microscope different than a light microscope? - ANS the e- replace the
light
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ANSWERS GRADED A+ 2025/2026
What is required by viruses? - ANS they must be inside a cell to replicate and are called
obligate intracellular parasites
What are 75% of the 80 new diseases discovered between 1980 and 2007? - ANS RNA viruses
What does it mean to be alive? - ANS It means to have a metabolism, to use or produce
energy, and to replicate
From the meaning behind "being alive", what all do viruses do? - ANS they replicate,
however they do not have a metabolism and they do not use or produce energy
What are the 3 domains used to classify microbes? - ANS 1. Bacteria
2. Archaea
3. Eukarya
Are there more humans or microbes on Earth? - ANS microbes
In the 3 domains used to classify microbes, how do the domains differ? - ANS they differ by
having different ribosomal RNA sequences
1 @COPYRIGHT 2026 ALLRIGHTS RESERVED.
,What microbes do not fall in the 3 domains of classifying microbes? - ANS Viruses and Prions
Which of the following are eukaryotic microbes?
A. Viruses
B. Bacteria
C. Animals
D. Yeast
E. C and D - ANS D, not A or B because they are not eukaryotic, and not C because animals
are not microbes
Viruses are classified in which domain?
A. Bacteria
B. Archaea
C. Eukarya
D. Viralae
E. None of the above - ANS E, because D does not exist as a domain and viruses do not fall in
A, B, or C
What is the magnification on a microscope? - ANS the objective lens x the eyepiece
What is the objective lens on a microscope? - ANS the specific magnifications - 10X, 40X,
100X - with X meaning the total magnification will be multiplied by 10 so for example 10X total
magnification will be 100
What is the eyepiece on a microscope? - ANS the thing you look through to view the microbe
which automatically magnifies by 10X
2 @COPYRIGHT 2026 ALLRIGHTS RESERVED.
,What is resolution when referring to a microscope? - ANS the ability of the lens to distinguish
between objects that are close together, also called the resolving power
What is the best light microscope resolution? - ANS 0.2 um
What is the diameter of a human cell and from this diameter could you see a human cell using a
light microscope? - ANS 10-100 um, yes you could see this on a light microscope
What is the diameter of a bacterial cell and from this diameter could you see it using a light
microscope? - ANS 1 um, yes you could see this on a light microscope, however only to see
shape, size, and arrangement of bacteria
What is the diameter of a virus and from this diameter could you see it using a light
microscope? - ANS 0.01 - 0.1 um, viruses are too small to see with a light microscope
What are the general sizes from biggest to largest of human cells, bacterial cells, and viruses? -
ANS Human Cells > Bacterial Cells > Viruses
What do you see when looking through the eyepiece of a bright field microscope? - ANS the
backfield will be bright and white and the specimen you're studying will be the color you stain
it, otherwise the specimen will be clear
What is the total magnification? - ANS objective lens x 10
What does a bright field microscope require? - ANS it requires heat-fix and stained bacteria
3 @COPYRIGHT 2026 ALLRIGHTS RESERVED.
, What does it mean to heat fix a smear? - ANS it means to pass the slide through a flame
which kills them and makes the bacteria stick to the slide
What do you see when looking through the eyepiece of a dark field microscope? - ANS light is
refracted by the organism, so the organism is bright and the backfield is dark
What does a dark field microscope require? - ANS nothing, heat-fix and stain is not required
so you can look at live organisms
What do you see when looking through the eyepiece of a phase contrast microscope? -
ANS it converts slight differences in refractive indexes into easily detected variations in light
intensity so you can look at live cells intracellular structures or intracellular processes such as
phagocytosis
How does a fluorescent microscope work? - ANS it uses a UV light to excite fluorescent
molecules such as green fluorescent protein or fluorochrome labeled antibodies
What is a way that a fluorescent microscope can be used not involving antibodies? - ANS The
organism takes up fluorescent dye directly such as green fluorescent protein and the dead cells
take up red dye
What is a way that a fluorescent microscope can be used involving antibodies? - ANS bacteria
can be linked to antibodies which are linked to fluorochrome which is a fluorescent molecule
Describe the resolution difference between a light microscope and an electron microscope? -
ANS an electron microscope resolution is 1000x better than a light microscope (5 x 10^-4 um)
How is an electron microscope different than a light microscope? - ANS the e- replace the
light
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