Whats Included ?
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Complete Exam Guide
,TABLE OF CONTENTS
Nucleic Acids
Genotype to Phenotype
Gene Expression and Epigenetics
Collection, Isolation and Quantification of Nucleic Acids
Resolution and Detection of Nucleic Acids
Analysis and Characterization of Nucleic Acids and Proteins
Nucleic Acid Amplification
Human Chromosomal Structure and Structural Variants
Targeted Variant Detection
Molecular Sequencing
Molecular Variants and Human Identification
Detection and Identification of Microorganisms
Molecular Detection of Inherited Diseases
Molecular Oncology
DNA-Based Tissue Typing
Quality Assurance and Quality Control in the Molecular Laboratory
,Chapter 1: Nucleic Acids
Multiple Choice
1. DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent
bond?
A. Aldehyde
B. Ester
C. Phosphodiester
D. Peptide
ANS: C
OBJ: 1.3
2. Nucleotides in DNA consist of which of the following?
A. Nitrogen base, deoxyribose, and phosphate
B. Nitrogen base, ribose, and sulfur
C. Carbon base, ribose, and phosphate
D. Carbon base, glucose, and carboxyl
ANS: A
OBJ: 1.1
3. Which of the five ribose carbons of the deoxyribonucleotide are involved in the formation of
the DNA chain?
A. 1' and 5'
B. 2' and 4'
C. 3' and 4'
D. 3' and 5'
ANS. D
OBJ: 1.2
4. In a DNA molecule, one end has a free hydroxyl group. What does the other end have?
A. A free phosphate group
B. A free hydroxyl group
C. A free amino group
D. A free carboxyl group.
ANS: A
OBJ: 1.2
Buckingham 4e, TB Ch 1, Page 1 of 11 Copyright © 2026
, 5. What type of bonds spontaneously form between two complementary strands of DNA?
A. Hydrogen bonds
B. Covalent bonds
C. Phosphodiester bonds
D. Hydrophobic bonds
ANS: A
OBJ: 1.2
6. In a double-stranded DNA molecule, base pairing between strands occurs between which of
the following?
A. A purine and a purine
B. A pyrimidine and a pyrimidine
C. A purine and a pyrimidine
D. All types of nucleotide bases
ANS: C
OBJ: 1.4
7. Which of the following is a purine?
A. Tyrosine
B. Cytosine
C. Adenine
D. Alanine
ANS: C
OBJ: 1.1
8. Which DNA sequence is complementary to 5'GATTCTCAAAGGACT3'?
A. 5'GATTCTCAAAGGACT3'
B. 3'GATTCTCAAAGGACT5'
C. 3'CTAAGAGTTTCCTGA5'
D. 5'CTAAGAGTTTCCTGA3'
ANS: C
OBJ: 1.5
9. Which term describes the arrangement of the individual strands in the double-stranded DNA
molecule?
A. Parallel
B. Antiparallel
C. Tangential
D. Divergent
ANS: B
OBJ: 1.5
Buckingham 4e, TB Ch 1, Page 2 of 11 Copyright © 2026
All Chapters test bank
Verified Answers
Complete Exam Guide
,TABLE OF CONTENTS
Nucleic Acids
Genotype to Phenotype
Gene Expression and Epigenetics
Collection, Isolation and Quantification of Nucleic Acids
Resolution and Detection of Nucleic Acids
Analysis and Characterization of Nucleic Acids and Proteins
Nucleic Acid Amplification
Human Chromosomal Structure and Structural Variants
Targeted Variant Detection
Molecular Sequencing
Molecular Variants and Human Identification
Detection and Identification of Microorganisms
Molecular Detection of Inherited Diseases
Molecular Oncology
DNA-Based Tissue Typing
Quality Assurance and Quality Control in the Molecular Laboratory
,Chapter 1: Nucleic Acids
Multiple Choice
1. DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent
bond?
A. Aldehyde
B. Ester
C. Phosphodiester
D. Peptide
ANS: C
OBJ: 1.3
2. Nucleotides in DNA consist of which of the following?
A. Nitrogen base, deoxyribose, and phosphate
B. Nitrogen base, ribose, and sulfur
C. Carbon base, ribose, and phosphate
D. Carbon base, glucose, and carboxyl
ANS: A
OBJ: 1.1
3. Which of the five ribose carbons of the deoxyribonucleotide are involved in the formation of
the DNA chain?
A. 1' and 5'
B. 2' and 4'
C. 3' and 4'
D. 3' and 5'
ANS. D
OBJ: 1.2
4. In a DNA molecule, one end has a free hydroxyl group. What does the other end have?
A. A free phosphate group
B. A free hydroxyl group
C. A free amino group
D. A free carboxyl group.
ANS: A
OBJ: 1.2
Buckingham 4e, TB Ch 1, Page 1 of 11 Copyright © 2026
, 5. What type of bonds spontaneously form between two complementary strands of DNA?
A. Hydrogen bonds
B. Covalent bonds
C. Phosphodiester bonds
D. Hydrophobic bonds
ANS: A
OBJ: 1.2
6. In a double-stranded DNA molecule, base pairing between strands occurs between which of
the following?
A. A purine and a purine
B. A pyrimidine and a pyrimidine
C. A purine and a pyrimidine
D. All types of nucleotide bases
ANS: C
OBJ: 1.4
7. Which of the following is a purine?
A. Tyrosine
B. Cytosine
C. Adenine
D. Alanine
ANS: C
OBJ: 1.1
8. Which DNA sequence is complementary to 5'GATTCTCAAAGGACT3'?
A. 5'GATTCTCAAAGGACT3'
B. 3'GATTCTCAAAGGACT5'
C. 3'CTAAGAGTTTCCTGA5'
D. 5'CTAAGAGTTTCCTGA3'
ANS: C
OBJ: 1.5
9. Which term describes the arrangement of the individual strands in the double-stranded DNA
molecule?
A. Parallel
B. Antiparallel
C. Tangential
D. Divergent
ANS: B
OBJ: 1.5
Buckingham 4e, TB Ch 1, Page 2 of 11 Copyright © 2026
