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STRAIGHTERLINE BIO 250 Lab
2 Culturing & Aseptic Technique
BIO250L Latest updated version
with 100% accuracy
Student Name: PRISCA EHIZIBUE
Access Code (located on the lid of your lab kit): BIO250L - KIT6250
Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this
worksheet.
Submissions without proper formatting, all required photos or sufficient responses
will be rejected.
Pre-lab Questions
1. This lab includes two experiments that each look at different aspects
of culturing microorganisms onto growth media. What concepts does
each experiment focus on? Why do you think these concepts are
important to the field of microbiology?
The reason of practice one is that is remains the most important
objective in the control of aseptic procedures. cultural due to agar
media and inoculation. What is emphasized here is the production of
strategies. Bacteria or other Microbes using nutrients in order to grow
and reproduce in the area of the nutrient agar centime containers. By
this, the participants are informed and motivated to combat climate
, FOR MORE EXAMS
EMAIL:
change. The competency of growing bacteria under controlled and
hygienic environment as one of the components. Apart from this, it is
necessary to do experimentation two in many circumstances of
microbiology studies from the pure laboratory to the diagnostic
services in hospitals. microbial load in the region from therapeutic
interventions to the diagnostic equipment. It is in this era that
punishment is secured in skills learnt. They are the basic approaches
used in the study of microbiology and they are the ways by which
different aspects of microbiology are studied and understood.
although it is a fundamental and a powerful constituent of many
investigations in the field of microbiology, it is also very general and
important in microbial analysis and processes.
2. In this lab, the experiments will allow or call for the use of four
inoculation tools. List these tools and provide a scenario in which
you would use each tool.
○ Micropipette: A micropipette is crucial in reminding small
cultures of bacteria; especially when in different serial dilutions
that is used in experimentations that need certain angles of
engagement.
○ It is used to separate bacterial cells from fluid culture so that
they could be concentrated and/or activated for subsequent
work such as isolation of DNA.
○ Inoculation of bacterial cultures on agar plates is done by
streaking using a loop or wire needle which helps in having
single isolated colonies. It helps in identification of the pure
bacterial cultures to be used in other tests..
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EMAIL:
○ A spreader assists in the distribution of bacterial cultures on
exteriors of agar which is useful for growth. This backs up the
research on the capacity of bacteria to the antimicrobial
compounds.
3. Describe a piece of equipment used in biotech labs to extend the
growth pattern of microbes.
Focus particularly on those used with E. coli used to make human
insulin.
Some of the essential apparatuses of the biotech lab for the microbe E.
coli, which has been used in the synthesis of human insulin, are a
bioreactor. It refers to the optimum condition & situation that is the
best when temp is constant & nutrients are available. , increasing the
variety of microbes, promoting the industrialization, and changing
the method of cultivation.
4. You’re a physician trying to isolate bacterial colonies from the human
gut in an attempt to diagnose a gastrointestinal infection. You streak
your sample on a growth media containing glucose, amino acids, and
salts containing both sulfur and phosphorus with a pH of 7. You
incubate the plates in aerobic conditions at 37 ˚C for three days, at
which point you can see clear bacterial colonies forming on the plate.
Would you feel confident in stating that you had successfully
cultured all the bacteria from your gut sample? Why or why not?
It is safer to use a positive sign that not all the gut bacteria have been
produced, and this is the colour of the normal colony of microbes in
selective media. It is therefore probable that some species may face
EMAIL:
STRAIGHTERLINE BIO 250 Lab
2 Culturing & Aseptic Technique
BIO250L Latest updated version
with 100% accuracy
Student Name: PRISCA EHIZIBUE
Access Code (located on the lid of your lab kit): BIO250L - KIT6250
Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this
worksheet.
Submissions without proper formatting, all required photos or sufficient responses
will be rejected.
Pre-lab Questions
1. This lab includes two experiments that each look at different aspects
of culturing microorganisms onto growth media. What concepts does
each experiment focus on? Why do you think these concepts are
important to the field of microbiology?
The reason of practice one is that is remains the most important
objective in the control of aseptic procedures. cultural due to agar
media and inoculation. What is emphasized here is the production of
strategies. Bacteria or other Microbes using nutrients in order to grow
and reproduce in the area of the nutrient agar centime containers. By
this, the participants are informed and motivated to combat climate
, FOR MORE EXAMS
EMAIL:
change. The competency of growing bacteria under controlled and
hygienic environment as one of the components. Apart from this, it is
necessary to do experimentation two in many circumstances of
microbiology studies from the pure laboratory to the diagnostic
services in hospitals. microbial load in the region from therapeutic
interventions to the diagnostic equipment. It is in this era that
punishment is secured in skills learnt. They are the basic approaches
used in the study of microbiology and they are the ways by which
different aspects of microbiology are studied and understood.
although it is a fundamental and a powerful constituent of many
investigations in the field of microbiology, it is also very general and
important in microbial analysis and processes.
2. In this lab, the experiments will allow or call for the use of four
inoculation tools. List these tools and provide a scenario in which
you would use each tool.
○ Micropipette: A micropipette is crucial in reminding small
cultures of bacteria; especially when in different serial dilutions
that is used in experimentations that need certain angles of
engagement.
○ It is used to separate bacterial cells from fluid culture so that
they could be concentrated and/or activated for subsequent
work such as isolation of DNA.
○ Inoculation of bacterial cultures on agar plates is done by
streaking using a loop or wire needle which helps in having
single isolated colonies. It helps in identification of the pure
bacterial cultures to be used in other tests..
, FOR MORE EXAMS
EMAIL:
○ A spreader assists in the distribution of bacterial cultures on
exteriors of agar which is useful for growth. This backs up the
research on the capacity of bacteria to the antimicrobial
compounds.
3. Describe a piece of equipment used in biotech labs to extend the
growth pattern of microbes.
Focus particularly on those used with E. coli used to make human
insulin.
Some of the essential apparatuses of the biotech lab for the microbe E.
coli, which has been used in the synthesis of human insulin, are a
bioreactor. It refers to the optimum condition & situation that is the
best when temp is constant & nutrients are available. , increasing the
variety of microbes, promoting the industrialization, and changing
the method of cultivation.
4. You’re a physician trying to isolate bacterial colonies from the human
gut in an attempt to diagnose a gastrointestinal infection. You streak
your sample on a growth media containing glucose, amino acids, and
salts containing both sulfur and phosphorus with a pH of 7. You
incubate the plates in aerobic conditions at 37 ˚C for three days, at
which point you can see clear bacterial colonies forming on the plate.
Would you feel confident in stating that you had successfully
cultured all the bacteria from your gut sample? Why or why not?
It is safer to use a positive sign that not all the gut bacteria have been
produced, and this is the colour of the normal colony of microbes in
selective media. It is therefore probable that some species may face
