Page | 1
BIOL 200 - ANKI IMPORT 2026
LATEST QUESTIONS AND
ANSWERS| ACE YOUR GRADES.
Next generation sequencing techniques - correct answer -- ex.
Illumina sequencing, does a PCR in a solid support to obtain lots
of copies of the same molecule in a very small space. They use
these DNA clusters for sequencing! - some sequencing skips
PCR step, such as Nanopore sequencing
Nanopore Sequencing Advantages - correct answer --
sequencing single molecules leads to possibility of new biological
questions - very long readability minimize need for genome
assembly, allows mapping of repetitive sequences - portable!
small! can be read on a laptop!
Nanopore Sequencing - correct answer -- involves pore protein -
electrical current is sent through pore, as current changes it
indicates different bases pass through - can read sequence based
on electrical output
, Page | 2
Illumina Sequencing - correct answer -1. Double strand DNA is
cut, only one strand is sequenced 2. New primer is added 3. One
fluorescently labelled dNTPs is bound, rest are washed away.
Imaging is determined. 4. Fluorophore from dNTP removed
chemically, base stays. No longer a terminator (reversible
terminator). 5. Repeat DNA until strand is replicated! 6. Look at
illumina. Each point represents different DNA molecule being
sequenced, can read many different sequences at the same time!
Genome - correct answer -- The entirety of an organism's
hereditary information - Usually DNA (but some viruses have RNA
genomes). *plants have chloroplast DNA too, animals have
mitochondrial
Genome Size - correct answer -Number of DNA base pairs in an
organism
Number of Genes - correct answer -Number of coding regions
within DNA (number of genes)
True/false: Biological complexity is related to the DNA content in
the genome. - correct answer -False
, Page | 3
How many base pairs is the human genome? - correct answer -
3300 Mbp *(3300 million base pairs)
As of 2021, the genomes of [...] species of animals have been
sequenced - correct answer -As of 2021, the genomes of 3278
species of animals have been sequenced
Exact Definition of a Gene - correct answer -"the entire nucleic
acid sequence that is necessary for the synthesis of a functional
product (polypeptide or RNA). *some genes encode for RNA's,
some function as components of a ribosome"
Introns - correct answer -DNA segments that separate the exons
and are spliced out during mRNA processing.
Exons - correct answer -Exons of a gene contain the coding
region or Open Reading Frame (ORF)
, Page | 4
Control Regions - correct answer -interact with factors to control
gene expression. They containe promoters and cis-regulatory
factors
Genes can be considered as [...] - correct answer -Genes can be
considered as transcription units
BLAST for protein sequencing - correct answer -- nucleic acid
and protein sequence similarity can be found using BLAST (Basic
Local Alignment Search Tool) - Proteins with similar functions
often contain similar amino acid sequences that encode functional
domains - first step when figuring out what genes code for
NF1 and BLAST - correct answer -- using BLAST, found
mutation in NF1 causes problem with neurofibromin 1 protein
which was associated with cancer - found similarities for IRA
yeast protein, a GTPase activating protein - IRA protein turns off
Ras, which normally regulates cell division.
Difference in genome size among species is mostly due to
different amounts of non- coding DNA, not related to number of
coding regions. Protein number varies much [...] among species
than DNA content. - correct answer -Difference in genome size
BIOL 200 - ANKI IMPORT 2026
LATEST QUESTIONS AND
ANSWERS| ACE YOUR GRADES.
Next generation sequencing techniques - correct answer -- ex.
Illumina sequencing, does a PCR in a solid support to obtain lots
of copies of the same molecule in a very small space. They use
these DNA clusters for sequencing! - some sequencing skips
PCR step, such as Nanopore sequencing
Nanopore Sequencing Advantages - correct answer --
sequencing single molecules leads to possibility of new biological
questions - very long readability minimize need for genome
assembly, allows mapping of repetitive sequences - portable!
small! can be read on a laptop!
Nanopore Sequencing - correct answer -- involves pore protein -
electrical current is sent through pore, as current changes it
indicates different bases pass through - can read sequence based
on electrical output
, Page | 2
Illumina Sequencing - correct answer -1. Double strand DNA is
cut, only one strand is sequenced 2. New primer is added 3. One
fluorescently labelled dNTPs is bound, rest are washed away.
Imaging is determined. 4. Fluorophore from dNTP removed
chemically, base stays. No longer a terminator (reversible
terminator). 5. Repeat DNA until strand is replicated! 6. Look at
illumina. Each point represents different DNA molecule being
sequenced, can read many different sequences at the same time!
Genome - correct answer -- The entirety of an organism's
hereditary information - Usually DNA (but some viruses have RNA
genomes). *plants have chloroplast DNA too, animals have
mitochondrial
Genome Size - correct answer -Number of DNA base pairs in an
organism
Number of Genes - correct answer -Number of coding regions
within DNA (number of genes)
True/false: Biological complexity is related to the DNA content in
the genome. - correct answer -False
, Page | 3
How many base pairs is the human genome? - correct answer -
3300 Mbp *(3300 million base pairs)
As of 2021, the genomes of [...] species of animals have been
sequenced - correct answer -As of 2021, the genomes of 3278
species of animals have been sequenced
Exact Definition of a Gene - correct answer -"the entire nucleic
acid sequence that is necessary for the synthesis of a functional
product (polypeptide or RNA). *some genes encode for RNA's,
some function as components of a ribosome"
Introns - correct answer -DNA segments that separate the exons
and are spliced out during mRNA processing.
Exons - correct answer -Exons of a gene contain the coding
region or Open Reading Frame (ORF)
, Page | 4
Control Regions - correct answer -interact with factors to control
gene expression. They containe promoters and cis-regulatory
factors
Genes can be considered as [...] - correct answer -Genes can be
considered as transcription units
BLAST for protein sequencing - correct answer -- nucleic acid
and protein sequence similarity can be found using BLAST (Basic
Local Alignment Search Tool) - Proteins with similar functions
often contain similar amino acid sequences that encode functional
domains - first step when figuring out what genes code for
NF1 and BLAST - correct answer -- using BLAST, found
mutation in NF1 causes problem with neurofibromin 1 protein
which was associated with cancer - found similarities for IRA
yeast protein, a GTPase activating protein - IRA protein turns off
Ras, which normally regulates cell division.
Difference in genome size among species is mostly due to
different amounts of non- coding DNA, not related to number of
coding regions. Protein number varies much [...] among species
than DNA content. - correct answer -Difference in genome size
