ASCP MB Molecular Biology Exam
Questions and Answers 100%
PASS
RNA Pol II—ANSWER-This polymerase acts on DNA and produces Messenger RNA.
1691G>A—ANSWER-Mutation associated with deep venous thrombosis.
Next Generation Sequencing—ANSWER-Uses short sequence reads.
KRAS, EGFR, ALK—ANSWER-Genes screened in a lung cancer panel.
DNA Polymerase III—ANSWER-Responsible for copying DNA by reading existing strand,
building new complementary strand and always adds to 3' end, but can't start a new strand
on its own.
3'-GAC—ANSWER-Palindrome in the restriction enzyme site: 5'-CTGCAG-3'.
Non-Hodgkin Lymphoma—ANSWER-Malignancy associated with T-Cell Gene
Rearrangement.
Splicing—ANSWER-The process that removes introns and preserves exons.
Nested PCR—ANSWER-PCR method with the highest specificity.
5'-ATCTATGTCGGCAATT-3'—ANSWER-Appropriate sense strand for the anti-sense strand of
DNA: 5'-AATTGCCGACATAGAT-3'.
, Beta—ANSWER-Phosphate of an incoming nucleotide attacked by the 3' -OH of the growing
DNA chain.
-70ºC—ANSWER-Optimal storage option for isolated DNA for a period greater than seven
years.
DNA Ligase—ANSWER-Enzyme involved in LCR.
Strict promoter sequences—ANSWER-Not a characteristic of eukaryotic DNA transcription.
Sigma subunit—ANSWER-Subunit of RNA polymerase holoenzyme responsible for promoter
recognition.
HCV type 1 & 4—ANSWER-Responds poorly to Ribavarin/Interferon treatment.
Type III—ANSWER-Restriction enzyme that digests and adds a methyl group from Adenine.
Type I—ANSWER-Restriction enzyme that digests and removes a methyl group from
Adenine.
PCR-RFLP—ANSWER-Best method to detect the Leiden mutation.
Initiation, exponential, plateau—ANSWER-Phases in a qPCR Amplification Plot.
RNA Pol I—ANSWER-This polymerase acts on DNA and produces Ribosomal RNA.
t(9;22)—ANSWER-Translocation that creates a fusion protein resulting in chronic
myelogenous leukemia (CML).
KRAS, BRAF, PIK3CA—ANSWER-Genes screened in a colorectal panel.
TATAAT—ANSWER-Sequence of the -10 region of prokaryotic promoters.
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
Questions and Answers 100%
PASS
RNA Pol II—ANSWER-This polymerase acts on DNA and produces Messenger RNA.
1691G>A—ANSWER-Mutation associated with deep venous thrombosis.
Next Generation Sequencing—ANSWER-Uses short sequence reads.
KRAS, EGFR, ALK—ANSWER-Genes screened in a lung cancer panel.
DNA Polymerase III—ANSWER-Responsible for copying DNA by reading existing strand,
building new complementary strand and always adds to 3' end, but can't start a new strand
on its own.
3'-GAC—ANSWER-Palindrome in the restriction enzyme site: 5'-CTGCAG-3'.
Non-Hodgkin Lymphoma—ANSWER-Malignancy associated with T-Cell Gene
Rearrangement.
Splicing—ANSWER-The process that removes introns and preserves exons.
Nested PCR—ANSWER-PCR method with the highest specificity.
5'-ATCTATGTCGGCAATT-3'—ANSWER-Appropriate sense strand for the anti-sense strand of
DNA: 5'-AATTGCCGACATAGAT-3'.
, Beta—ANSWER-Phosphate of an incoming nucleotide attacked by the 3' -OH of the growing
DNA chain.
-70ºC—ANSWER-Optimal storage option for isolated DNA for a period greater than seven
years.
DNA Ligase—ANSWER-Enzyme involved in LCR.
Strict promoter sequences—ANSWER-Not a characteristic of eukaryotic DNA transcription.
Sigma subunit—ANSWER-Subunit of RNA polymerase holoenzyme responsible for promoter
recognition.
HCV type 1 & 4—ANSWER-Responds poorly to Ribavarin/Interferon treatment.
Type III—ANSWER-Restriction enzyme that digests and adds a methyl group from Adenine.
Type I—ANSWER-Restriction enzyme that digests and removes a methyl group from
Adenine.
PCR-RFLP—ANSWER-Best method to detect the Leiden mutation.
Initiation, exponential, plateau—ANSWER-Phases in a qPCR Amplification Plot.
RNA Pol I—ANSWER-This polymerase acts on DNA and produces Ribosomal RNA.
t(9;22)—ANSWER-Translocation that creates a fusion protein resulting in chronic
myelogenous leukemia (CML).
KRAS, BRAF, PIK3CA—ANSWER-Genes screened in a colorectal panel.
TATAAT—ANSWER-Sequence of the -10 region of prokaryotic promoters.
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
