ASCP MB: Extra-Practice
Questions WITH Answers 100%
PASS
The Klenow fragment of E. coli DNA polymerase I is used:—ANSWER-to label DNA probes by
random primer labeling.
What is a solution hybridization method for the detection of nucleic acid:protein
interactions?—ANSWER-gel mobility shift assay
_____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific
interaction occurs, visible with an apparent increase in molecular size on a gel due to the
protein:nucleic acid interaction.—ANSWER-Gel mobility shift assay
Which probe is most often used to detect trisomy 21 by interphase FISH?—ANSWER-alpha
satellite DNA probe
_____ detect specific chromosomes and numbers of the specific chromosomes.—ANSWER-
Alpha satellite DNA probe
Which reagent generates a signal during the annealing stage of a quantitative real time PCR
reaction?—ANSWER-molecular beacon
Methylation of cytosine residues is involved in the alteration of gene expression in a number
of cancers. Which sequencing technique is best suited to determine if methylation of
, cytosine residues has taken place in the promoter region of a given gene?—ANSWER-
bisulfite sequencing
_____ is a modified chain terminator method specifically designed to detect methylated
nucleotides.—ANSWER-Bisulfite sequencing
Which method is best suited to screen for mutations in a gene having multiple possible
mutations?—ANSWER-single strand conformational polymorphism (SSCP)
_____ is a screening test in which the exact nature of a mutation does not need to be known
in order to detect the presence of a mutation.—ANSWER-Single strand conformational
polymorphism (SSCP)
What is the best method to rule out the possibility of genomic DNA giving a false-positive
result in an RT-PCR reaction?—ANSWER-use of upstream and downstream primers that span
an exon-intron-exon region of the target
*amplicaons generated from genomic DNA would be larger than the specific amplicon
generated from a mature, spliced mRNA
A common use for pulsed-field gel electrophoresis is:—ANSWER-epidemiological typing of
bacterial strains.
_____ after digestion of bacterial chromosomes with restriction enzymes is used to
determine similarity between bacterial isolates.—ANSWER-PFGE
A translocation in which chromosome pair creates a BCR/ABL1 fusion gene product
associated with CML?
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
Questions WITH Answers 100%
PASS
The Klenow fragment of E. coli DNA polymerase I is used:—ANSWER-to label DNA probes by
random primer labeling.
What is a solution hybridization method for the detection of nucleic acid:protein
interactions?—ANSWER-gel mobility shift assay
_____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific
interaction occurs, visible with an apparent increase in molecular size on a gel due to the
protein:nucleic acid interaction.—ANSWER-Gel mobility shift assay
Which probe is most often used to detect trisomy 21 by interphase FISH?—ANSWER-alpha
satellite DNA probe
_____ detect specific chromosomes and numbers of the specific chromosomes.—ANSWER-
Alpha satellite DNA probe
Which reagent generates a signal during the annealing stage of a quantitative real time PCR
reaction?—ANSWER-molecular beacon
Methylation of cytosine residues is involved in the alteration of gene expression in a number
of cancers. Which sequencing technique is best suited to determine if methylation of
, cytosine residues has taken place in the promoter region of a given gene?—ANSWER-
bisulfite sequencing
_____ is a modified chain terminator method specifically designed to detect methylated
nucleotides.—ANSWER-Bisulfite sequencing
Which method is best suited to screen for mutations in a gene having multiple possible
mutations?—ANSWER-single strand conformational polymorphism (SSCP)
_____ is a screening test in which the exact nature of a mutation does not need to be known
in order to detect the presence of a mutation.—ANSWER-Single strand conformational
polymorphism (SSCP)
What is the best method to rule out the possibility of genomic DNA giving a false-positive
result in an RT-PCR reaction?—ANSWER-use of upstream and downstream primers that span
an exon-intron-exon region of the target
*amplicaons generated from genomic DNA would be larger than the specific amplicon
generated from a mature, spliced mRNA
A common use for pulsed-field gel electrophoresis is:—ANSWER-epidemiological typing of
bacterial strains.
_____ after digestion of bacterial chromosomes with restriction enzymes is used to
determine similarity between bacterial isolates.—ANSWER-PFGE
A translocation in which chromosome pair creates a BCR/ABL1 fusion gene product
associated with CML?
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
