ASCP Study Exam Questions
WITH Answers 100% PASS
What is metagenome?—ANSWER-Sampling the genome sequence of a community of
organisms.
Polymorphism involved for Irinotecan?—ANSWER-UGT1A1
NGS related to deletions and insertions, coverage, depth?—ANSWER--Paired-end reads for
detection of indels
-Coverage is how much of your target has been read
-Depth is how many times a unique position has been covered
Polymerase for lagging strand?—ANSWER-Euk - DNA Pol Alpha
Pro - DNA Pol I
Polymerase for leading strand?—ANSWER-Euk - DNA Pol Delta and DNA Pol Epsilon
Pro - DNA Pol III
Polymerase used in LAMP?—ANSWER--Loop-mediated Isothermal Amplification utilizes Bst
Polymerase
-Has helicase activity and activity at annealing/elongating temps
, Highest resistance gene in enterococcus?—ANSWER-vanA has the highest resistance in
enterococcus
Resistance in mecA gene in MRSA codes for?—ANSWER-mecA responsible for antibiotic
resistance in S. aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam
antibiotics like penicillin.
Important in gene expression, defense in eukaryotes?—ANSWER-Person guessed mRNA -
gene-expression and codes for proteins that bind to DNA to promote/inhibit expression.
Donor/Recipient - what's informative loci?—ANSWER-No matching alleles is informative, you
can tell D from R; non-informative would be both D&R matching.
Bond between DNA and histones?—ANSWER-Ionic - DNA is negative and histones are
positive
Amino Acids that make up histones?—ANSWER-Arginine and Lysine - positively charged
amino acids
Multiplex modifications for low yield?—ANSWER-Person guessed KCl.
Increasing Mg2+ could increase yield.
Decreasing stringency increases yield, but increases chances of mispriming
PCR troubleshooting—ANSWER-Increase Temp --> Increase Stringency
Increase Probe Length --> Increase Stringency
Increase Probe Complexity --> Increase Stringency
Lower Salt Conc. --> Increase Stringency
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
WITH Answers 100% PASS
What is metagenome?—ANSWER-Sampling the genome sequence of a community of
organisms.
Polymorphism involved for Irinotecan?—ANSWER-UGT1A1
NGS related to deletions and insertions, coverage, depth?—ANSWER--Paired-end reads for
detection of indels
-Coverage is how much of your target has been read
-Depth is how many times a unique position has been covered
Polymerase for lagging strand?—ANSWER-Euk - DNA Pol Alpha
Pro - DNA Pol I
Polymerase for leading strand?—ANSWER-Euk - DNA Pol Delta and DNA Pol Epsilon
Pro - DNA Pol III
Polymerase used in LAMP?—ANSWER--Loop-mediated Isothermal Amplification utilizes Bst
Polymerase
-Has helicase activity and activity at annealing/elongating temps
, Highest resistance gene in enterococcus?—ANSWER-vanA has the highest resistance in
enterococcus
Resistance in mecA gene in MRSA codes for?—ANSWER-mecA responsible for antibiotic
resistance in S. aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam
antibiotics like penicillin.
Important in gene expression, defense in eukaryotes?—ANSWER-Person guessed mRNA -
gene-expression and codes for proteins that bind to DNA to promote/inhibit expression.
Donor/Recipient - what's informative loci?—ANSWER-No matching alleles is informative, you
can tell D from R; non-informative would be both D&R matching.
Bond between DNA and histones?—ANSWER-Ionic - DNA is negative and histones are
positive
Amino Acids that make up histones?—ANSWER-Arginine and Lysine - positively charged
amino acids
Multiplex modifications for low yield?—ANSWER-Person guessed KCl.
Increasing Mg2+ could increase yield.
Decreasing stringency increases yield, but increases chances of mispriming
PCR troubleshooting—ANSWER-Increase Temp --> Increase Stringency
Increase Probe Length --> Increase Stringency
Increase Probe Complexity --> Increase Stringency
Lower Salt Conc. --> Increase Stringency
© 2026 Copyright. All Rights Reserved. This document is
protected by copyright law, Copyrighted By Brittie Donald
